Calorimetry

Prof. Andrew Feig joined the WSU faculty in the Department of Chemistry in 2006. He is a biochemist who oversees a laboratory that studies bacterial gene regulation by small non-coding RNAs and the biochemistry, chemical biology, and biophysics of Clostridium difficile toxins A & B. He has worked extensively on structural rearrangements of RNA and RNA protein interactions with an emphasis on the biochemistry and biophysics of those processes. He has a long-standing interested in heat capacity changes associated with these structural transitions and their impact on the regulation of biological processes. Professor Feig is a 2002 Cottrell Scholar, was Recipient of the WSU Career Development Chair (08/09), the CLAS Teaching Award (2012) and the WSU President’s Award for Excellence in Teaching (2013). In 2015 he was named PCSUM Michigan Distinguished Professor of the Year. Professor Feig earned his B.S. in Chemistry from Yale University (1990) and his Ph.D. in Chemistry from MIT (1995) working with Professor Stephen J. Lippard. He worked as a post-doctoral fellow at University of Colorado, Boulder (1995-1999) in the laboratory of Olke C. Uhlenbeck.

Praise for the Series: "Should be on the shelves of all libraries in the world as a whole collection." --Chemistry in Industry"The work most often consulted in the lab." --Enzymologia"The Methods in Enzymology series represents the gold-standard." --Neuroscience

• 1. Isothermal Titration Calorimetry Measurements of Metal Ions Binding to ProteinsColette F. Quinn ,Margaret C. Carpenter, Molly L. Croteau and Dean E. Wilcox2. Assessing Coupled Protein Folding and Binding through Temperature- Dependent Isothermal Titration CalorimetryDebashish Sahu, Monique Bastidas, Chad W. Lawrence, William G. Noid,and Scott A. Showalter3. Fragment-Based Screening for Enzyme Inhibitors Using CalorimetryMichael I. Recht, Vicki Nienaber and Francisco E. Torres4. Measuring Multivalent Binding Interactions by Isothermal Titration CalorimetryTarun K. Dam,  Melanie L Talaga, Ni Fan and Curtis F. Brewer5. Calorimetric and Spectroscopic Analysis of the Thermal Stability of Short Duplex DNA-Containing Sugar and Base-Modified NucleotidesKareem Fakhfakh, Curtis B. Hughesman, A. Louise Creagh, Vincent Kao and  Charles Haynes6. Calorimetric Quantification of Cyclodextrin-Mediated Detergent Extraction for Membrane-Protein ReconstitutionMartin Textor and Sandro Keller7. Extending ITC to Kinetics with KinITCPhilippe Dumas, Eric Ennifar, Cyrielle Da Veiga, Guillaume Bec, William Palau, Carmelo Di Primo,Angel Piñeiro, Juan Sabin, Eva Muñoz and Javier Rial8. Measuring the Kinetics of Molecular Association by Isothermal Titration Calorimetry Kirk A. Vander Meulen, Scott Horowitz, Raymond C. Trievel, and Samuel E. Butcher9. Isothermal Titration Calorimetry to Characterize Enzymatic ReactionsLuca Mazzei, Stefano Ciurli and Barbara Zambelli10. Avoiding Buffer Interference in Itc Experiments: A Case Study from the Analysis of  Entropy Driven Reactions of Glucose-6-Phosphate DehydrogenaseM. Lucia Bianconi11. ITC Methods for Assessing Buffer/Protein Interactions from the Perturbation of  Steady-State Kinetics: A Reactivity Study of Homoprotocatechuate 2,3-DioxygenaseKate L. Henderson, Delta K. Boyles, Vu H. Le, Edwin A. Lewis and Joseph P.Emerson12. Modern Analysis of Protein Folding by Differential Scanning CalorimetryBeatriz Ibarra-Molero, Athi N. Naganathan, Jose M. Sanchez-Ruiz and Victor Muñoz13. A Guide to Differential Scanning Calorimetry of Membrane and Soluble Proteins in DetergentsZhengrong Yang and Christie G. Brouillette14. Orthogonal Methods for Characterizing the Unfolding of Therapeutic Monoclonal  Antibodies: Differential Scanning Calorimetry, Isothermal Chemical Denaturation and  Intrinsic Fluorescence with Concomitant Static Light ScatteringDeniz B. Temel, Pavel Landsman and Mark L. Brader15. IATC, DSC, and PPC Analysis of Reversible and Multi-State Structural Transition of Cytochrome cShun-ichi Kidokoro and Shigeyoshi Nakamura16. The Complementarity of the Loop to the Stem in DNA Pseudoknots Gives Rise to Local TAT Base-TripletsCalliste Reiling-Steffensmeier and Luis A. Marky17. A High-Throughput Biological Calorimetry Core: Steps to Startup, Run, and Maintain a Multi-User FacilityNeela H. Yennawar, Julia A. Fecko, Scott A. Showalter and Philip C. Bevilacqua