Isotope Labeling of Biomolecules - Applications

Zvi Kelman is the Director of the Biomolecular Labeling Laboratory (BL2), National Institute of Standards and Technology (NIST). He is also an Adjunct Professor in the Department of Cell Biology and Molecular Genetics at the University of Maryland, College Park and affiliated with the Department of Biochemistry and Molecular Biology, University of Maryland School of Medicine. Zvi earned a B.Sc. in Agriculture from the Hebrew University in Jerusalem and a M.Sc. in Cell Biology from the Weizmann Institute of Science. After receiving a Ph.D. in Molecular Biology from Cornell University, he was a Helen Hay Whitney Foundation Post-Doctoral Fellow at Johns Hopkins University and Memorial Sloan-Kettering Cancer Center. Upon completion of his postgraduate training, he became a Life Technologies Professor at the Center for Advanced Research in Biotechnology (CARB), University of Maryland Biotechnology Institute (UMBI). Zvi moved to the University of Maryland, College Park in 2010 as a Professor in the Department of Cell Biology and Molecular Genetics. In 2011 he was recruited to NIST to direct the Biomolecular Labeling Laboratory.

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• Nuclear Magnetic Resonance Spectroscopy1. Application of Natural Isotopic Abundance 1H-13C and 1H-15N Correlated Two- Dimensional NMR for Evaluation of the Structure of Protein TherapeuticsLuke W. Arbogast, Robert G. Brinson and John P. Marino2. A Semi-Automated Assignment Protocol for Methyl Group Side-Chains in Large ProteinsJonggul Kim, Yingjie Wang, Geoffrey Li and Gianluigi Veglia3. 19F-site Specific Labeled Nucleotides for Nucleic Acid Structural Analysis by NMRLincoln G. Scott and Mirko Hennig4. Applying Thymine Isostere 2,4-Difluoro-5-methylbenzene as a NMR Assignment Tool and Probe of Homopyrimidine/Homopurine Tract Structural DynamicsRobert G. Brinson, Jennifer T. Miller, Jason D. Kahn, Stuart F. J. Le Grice and John P. MarinoScattering Techniques 5. Biomolecular Deuteration for Neutron Structural Biology and DynamicsMichael Haertlein, Martine Moulin, Juliette M. Devos, Valerie Laux, Orla Dunne, V. Trevor Forsyth6. Deuterium Labeling Together with Contrast Variation Small-angle Neutron Scattering Suggests How Skp Captures and Releases Unfolded Outer Membrane ProteinsNathan R. Zaccai, Clifford W. Sandlin, James T. Hoopes, Joseph E. Curtis, Patrick J. Fleming, Karen G. Fleming and Susan Krueger7. Deuteration in Biological Neutron ReflectometryFrank Heinrich8. Deuterium Labelling Strategies for Creating Contrast in Structure-Function Studies of Model Bacterial Outer Membranes Using Neutron Reflectometry Anton P. Le Brun, Luke A. Clifton, Stephen A. Holt, Peter J. Holden, and Jeremy H. Lakey9. Essential Strategies for Revealing Nanoscale Protein Dynamics By Neutron Spin Echo SpectroscopyDavid J. E. Callaway and Zimei BuMass Spectrometry10. Method for the Determination of 15N Incorporation Percentage in Labeled Peptides and ProteinsEric L. Kilpatrick11. QconCAT: Internal Standard for Protein QuantificationKerry Bauer Scott, Illarion V. Turko and Karen W. Phinney12. Production, Purification and Characterization of 15N-Labeled DNA Repair Proteins as Internal Standards for Mass Spectrometric MeasurementsPrasad T. Reddy, Pawel Jaruga, Bryant C. Nelson, Mark S. Lowenthal, Ann-Sofie Jemth, Olga Loseva, Erdem Coskun, Thomas Helleday and Miral DizdarogluHydrogen-Deuterium Exchange Mass Spectrometry13. Hydrogen Exchange Mass SpectrometryLeland Mayne14. Mapping Protein-Ligand Interactions with Proteolytic Fragmentation, Hydrogen/Deuterium Exchange-Mass SpectrometryElyssia S. Gallagher and Jeffrey W. Hudgens15. Isotope Labeling of Biomolecules “Structural analysis of viruses by HDX-MS"Miklos Guttman and Kelly K. Lee