Basic Science Methods for Clinical Researchers

Morteza Jalali is a British doctor-preneur who is fascinated by transformational healthcare innovation through synergies between clinicians, patients, industry and scientists. Born in Manchester, United Kingdom, at the age of 18 he commenced a medical degree at Manchester Medical School and undertook dedicated research training at The Healing Foundation Centre, University of Manchester, following the award of a Royal College of Surgeons student research scholarship. After the completion of his Bachelor’s, Master’s and medical degrees, Morteza decided to undertake a Doctor of Philosophy degree in stem cell biology at the Wellcome Trust-Medical Research Council Cambridge Stem Cell Institute and was awarded a Cambridge Cancer Centre clinical research training fellowship to study at Emmanuel College, University of Cambridge. Morteza is committing to a career as a surgeon scientist by undertaking a period of postdoctoral research in the United States. He is currently a surgeon in training (plastic surgery theme) and honorary research fellow at the University of Oxford where he enjoys fulfilling a variety of clinical teaching commitments, having been a former faculty member at the University of Bristol. Morteza enjoys rowing, amateur boxing, graphic design, and spending time with family and friends in the United Kingdom and abroad. Clinical Research Fellow (M.Phil.), Whitehead Institute for Biomedical Research, Massachusetts Institute of Technology, Cambridge, MA, USA Academic Foundation Doctor, Royal Liverpool University Hospital, Liverpool, UK

"The information provided would be an excellent tool for a clinician in training…the content is best suited for those clinicians who lack basic science training or a research background."--Annals of Allergy, Asthma & Immunology

• 1. The Polymerase Chain Reaction: PCR, qPCR, and RT-PCR2. Methods of Cloning3. Whole-Mount In Situ Hybridization and a Genotyping Method on Single Xenopus Embryos4. Microarrays: An Introduction and Guide to Their Use5. Analysis of Human Genetic Variations Using DNA Sequencing6. Western Blot7. The Enzyme-Linked Immunosorbent Assay: The Application of ELISA in Clinical Research8. Immunofluorescence9. Cell Culture: Growing Cells as Model Systems In Vitro10. Flow Cytometry11. Transfection12. In Vivo Animal Modeling: Drosophila13. Zebrafish as a Research Organism: Danio rerio in Biomedical Research14. Xenopus as a Model Organism for Biomedical Research15. Basic Mouse Methods for Clinician Researchers: Harnessing the Mouse for Biomedical Research