John T. Groves Collegiate Professor of Chemistry, Department of Chemistry, University of Michigan, USA

Praise for the Series: "Should be on the shelves of all libraries in the world as a whole collection." --Chemistry in Industry"The work most often consulted in the lab." --Enzymologia"The Methods in Enzymology series represents the gold-standard." --Neuroscience

• 1. Chemical Post-Translational Modification with Designed Rhodium(II) CatalystsSamuel C. Martin, Matthew B. Minus and Zachary T. Ball2. Cell Binding Assays for Determining the Affinity of Protein-Protein Interactions: Technologies and ConsiderationsSean Hunter and Jennifer Cochran3. Protein and Antibody Engineering by Phage DisplayJulia C. Frei and Jonathan R. Lai4. Incorporation of Unnatural Amino Acids into Proteins Expressed in Mammalian CellsRobert Serfling and Irene Coin5. Method for Enzyme Design with Genetically Encoded Unnatural Amino AcidsCheng Hu and Jiangyun Wang6. Methods for Solving Highly Symmetric De Novo Designed Metalloproteins: Crystallographic Examination of a Novel Three Stranded Coiled Coil Structure Containing D-Amino AcidsLeela Ruckthong, Jeanne A. Stuckey and Vincent L. Pecoraro7. SpyRings Declassified: A Blueprint for using Isopeptide-Mediated Cyclization to Enhance Enzyme Thermal ResilienceChristopher Schoene, S. Paul Bennett and Mark Howarth 8. Engineering and Application of LOV2-Based PhotoswitchesSeth Parker Zimmerman, Brian Kuhlman and Hayretin Yumerefendi9. Minimalist Design of Allosterically Regulated Protein CatalystsOlga V. Makhlynets and Ivan V. Korendovych10. Combining Design and Selection to Create Novel Protein-Peptide InteractionsElizabeth B. Speltz, Nicholas Sawyer and Lynne Regan 11. Metal-Directed Design of Supramolecular Protein AssembliesJake B. Bailey, Rohit H. Subramanian, Lewis A. Churchfield and F. Akif Tezcan12. Designing Fluorinated ProteinsE. Neil G. Marsh13. Solid Phase Synthesis of Helically Folded Aromatic OligoamidesSimon J. Dawson, Xiaobo Hu, Stijn Claerhout and Ivan Huc14. Conformational Restriction of Peptides Using Dithiol Bis-AlkylationLeila Peraro, Timothy R. Siegert and Joshua A. Kritzer15. Engineering Short Pre-Organized Peptide Sequences for Metal Ion Coordination: Copper(II) a Case StudyLuís M. P. Lima and Olga Iranzo16. De Novo Construction of Redox Active ProteinsC. C. Moser, M. M Sheehan, N. M. Ennist, G. Kodali, C. Bialas, M. T. Englander, B. M. Discher and P. L. Dutton17. Design Strategies for Redox Active Metalloenzymes: Applications in Hydrogen ProductionRafael Alcala-Torano, Dayn Joseph Sommer, Zahra Bahrami Dizicheh and Giovanna Ghirlanda18. Equilibrium Studies of Designed MetalloproteinsBrian R. Gibney19. Reconstitution of Heme Enzymes with Artificial MetalloporphyrinoidsKoji Oohora and Takashi Hayashi20. Creation of a Thermally Tolerant PeroxidaseYoshihito Watanabe and Hiroshi Nakajima21. Designing Covalently Linked Heterodimeric Four-Helix BundlesMarco Chino, Linda Leone, Ornella Maglio and Angela Lombardi22. Design of Heteronuclear MetalloenzymesAmbika Bhagi-Damodaran,Parisa Hosseinzadeh, Evan Mirts, Julian Reed, Igor D. Petrik and Yi Lu23. Periplasmic Screening for Artificial MetalloenzymesMarkus Jeschek, Sven Panke and Thomas R. Ward24. De Novo Designed Imaging Agents Based on Lanthanide Peptides ComplexesAnna F. A. Peacock25. Peptide Binding for Bio-Based NanomaterialsNicholas M. Bedford, Catherine Munro and Marc R. Knecht

John T. Groves Collegiate Professor of Chemistry, Department of Chemistry, University of Michigan, USA

Praise for the Series: "Should be on the shelves of all libraries in the world as a whole collection." --Chemistry in Industry"The work most often consulted in the lab." --Enzymologia"The Methods in Enzymology series represents the gold-standard." --Neuroscience

• 1. Chemical Post-Translational Modification with Designed Rhodium(II) CatalystsSamuel C. Martin, Matthew B. Minus and Zachary T. Ball2. Cell Binding Assays for Determining the Affinity of Protein-Protein Interactions: Technologies and ConsiderationsSean Hunter and Jennifer Cochran3. Protein and Antibody Engineering by Phage DisplayJulia C. Frei and Jonathan R. Lai4. Incorporation of Unnatural Amino Acids into Proteins Expressed in Mammalian CellsRobert Serfling and Irene Coin5. Method for Enzyme Design with Genetically Encoded Unnatural Amino AcidsCheng Hu and Jiangyun Wang6. Methods for Solving Highly Symmetric De Novo Designed Metalloproteins: Crystallographic Examination of a Novel Three Stranded Coiled Coil Structure Containing D-Amino AcidsLeela Ruckthong, Jeanne A. Stuckey and Vincent L. Pecoraro7. SpyRings Declassified: A Blueprint for using Isopeptide-Mediated Cyclization to Enhance Enzyme Thermal ResilienceChristopher Schoene, S. Paul Bennett and Mark Howarth 8. Engineering and Application of LOV2-Based PhotoswitchesSeth Parker Zimmerman, Brian Kuhlman and Hayretin Yumerefendi9. Minimalist Design of Allosterically Regulated Protein CatalystsOlga V. Makhlynets and Ivan V. Korendovych10. Combining Design and Selection to Create Novel Protein-Peptide InteractionsElizabeth B. Speltz, Nicholas Sawyer and Lynne Regan 11. Metal-Directed Design of Supramolecular Protein AssembliesJake B. Bailey, Rohit H. Subramanian, Lewis A. Churchfield and F. Akif Tezcan12. Designing Fluorinated ProteinsE. Neil G. Marsh13. Solid Phase Synthesis of Helically Folded Aromatic OligoamidesSimon J. Dawson, Xiaobo Hu, Stijn Claerhout and Ivan Huc14. Conformational Restriction of Peptides Using Dithiol Bis-AlkylationLeila Peraro, Timothy R. Siegert and Joshua A. Kritzer15. Engineering Short Pre-Organized Peptide Sequences for Metal Ion Coordination: Copper(II) a Case StudyLuís M. P. Lima and Olga Iranzo16. De Novo Construction of Redox Active ProteinsC. C. Moser, M. M Sheehan, N. M. Ennist, G. Kodali, C. Bialas, M. T. Englander, B. M. Discher and P. L. Dutton17. Design Strategies for Redox Active Metalloenzymes: Applications in Hydrogen ProductionRafael Alcala-Torano, Dayn Joseph Sommer, Zahra Bahrami Dizicheh and Giovanna Ghirlanda18. Equilibrium Studies of Designed MetalloproteinsBrian R. Gibney19. Reconstitution of Heme Enzymes with Artificial MetalloporphyrinoidsKoji Oohora and Takashi Hayashi20. Creation of a Thermally Tolerant PeroxidaseYoshihito Watanabe and Hiroshi Nakajima21. Designing Covalently Linked Heterodimeric Four-Helix BundlesMarco Chino, Linda Leone, Ornella Maglio and Angela Lombardi22. Design of Heteronuclear MetalloenzymesAmbika Bhagi-Damodaran,Parisa Hosseinzadeh, Evan Mirts, Julian Reed, Igor D. Petrik and Yi Lu23. Periplasmic Screening for Artificial MetalloenzymesMarkus Jeschek, Sven Panke and Thomas R. Ward24. De Novo Designed Imaging Agents Based on Lanthanide Peptides ComplexesAnna F. A. Peacock25. Peptide Binding for Bio-Based NanomaterialsNicholas M. Bedford, Catherine Munro and Marc R. Knecht

John T. Groves Collegiate Professor of Chemistry, Department of Chemistry, University of Michigan, USA

Praise for the Series: "Should be on the shelves of all libraries in the world as a whole collection." --Chemistry in Industry"The work most often consulted in the lab." --Enzymologia"The Methods in Enzymology series represents the gold-standard." --Neuroscience

• 1. Chemical Post-Translational Modification with Designed Rhodium(II) CatalystsSamuel C. Martin, Matthew B. Minus and Zachary T. Ball2. Cell Binding Assays for Determining the Affinity of Protein-Protein Interactions: Technologies and ConsiderationsSean Hunter and Jennifer Cochran3. Protein and Antibody Engineering by Phage DisplayJulia C. Frei and Jonathan R. Lai4. Incorporation of Unnatural Amino Acids into Proteins Expressed in Mammalian CellsRobert Serfling and Irene Coin5. Method for Enzyme Design with Genetically Encoded Unnatural Amino AcidsCheng Hu and Jiangyun Wang6. Methods for Solving Highly Symmetric De Novo Designed Metalloproteins: Crystallographic Examination of a Novel Three Stranded Coiled Coil Structure Containing D-Amino AcidsLeela Ruckthong, Jeanne A. Stuckey and Vincent L. Pecoraro7. SpyRings Declassified: A Blueprint for using Isopeptide-Mediated Cyclization to Enhance Enzyme Thermal ResilienceChristopher Schoene, S. Paul Bennett and Mark Howarth 8. Engineering and Application of LOV2-Based PhotoswitchesSeth Parker Zimmerman, Brian Kuhlman and Hayretin Yumerefendi9. Minimalist Design of Allosterically Regulated Protein CatalystsOlga V. Makhlynets and Ivan V. Korendovych10. Combining Design and Selection to Create Novel Protein-Peptide InteractionsElizabeth B. Speltz, Nicholas Sawyer and Lynne Regan 11. Metal-Directed Design of Supramolecular Protein AssembliesJake B. Bailey, Rohit H. Subramanian, Lewis A. Churchfield and F. Akif Tezcan12. Designing Fluorinated ProteinsE. Neil G. Marsh13. Solid Phase Synthesis of Helically Folded Aromatic OligoamidesSimon J. Dawson, Xiaobo Hu, Stijn Claerhout and Ivan Huc14. Conformational Restriction of Peptides Using Dithiol Bis-AlkylationLeila Peraro, Timothy R. Siegert and Joshua A. Kritzer15. Engineering Short Pre-Organized Peptide Sequences for Metal Ion Coordination: Copper(II) a Case StudyLuís M. P. Lima and Olga Iranzo16. De Novo Construction of Redox Active ProteinsC. C. Moser, M. M Sheehan, N. M. Ennist, G. Kodali, C. Bialas, M. T. Englander, B. M. Discher and P. L. Dutton17. Design Strategies for Redox Active Metalloenzymes: Applications in Hydrogen ProductionRafael Alcala-Torano, Dayn Joseph Sommer, Zahra Bahrami Dizicheh and Giovanna Ghirlanda18. Equilibrium Studies of Designed MetalloproteinsBrian R. Gibney19. Reconstitution of Heme Enzymes with Artificial MetalloporphyrinoidsKoji Oohora and Takashi Hayashi20. Creation of a Thermally Tolerant PeroxidaseYoshihito Watanabe and Hiroshi Nakajima21. Designing Covalently Linked Heterodimeric Four-Helix BundlesMarco Chino, Linda Leone, Ornella Maglio and Angela Lombardi22. Design of Heteronuclear MetalloenzymesAmbika Bhagi-Damodaran,Parisa Hosseinzadeh, Evan Mirts, Julian Reed, Igor D. Petrik and Yi Lu23. Periplasmic Screening for Artificial MetalloenzymesMarkus Jeschek, Sven Panke and Thomas R. Ward24. De Novo Designed Imaging Agents Based on Lanthanide Peptides ComplexesAnna F. A. Peacock25. Peptide Binding for Bio-Based NanomaterialsNicholas M. Bedford, Catherine Munro and Marc R. Knecht