Patricia H. Reggio is Marie Foscue Rourk Professsor and Head of the Department of Chemistry and Biochemistry, UNC-Greensboro, Greensboro, USA.

Praise for the Series: "Should be on the shelves of all libraries in the world as a whole collection." --Chemistry in Industry"The work most often consulted in the lab." --Enzymologia"The Methods in Enzymology series represents the gold-standard." --Neuroscience

• 1. Mouse Neuroblastoma CB1 Cannabinoid Receptor-Stimulated [35S]GTPɣS Binding: Total and Antibody-Targeted Gα Protein-Specific Scintillation Proximity Assays2. Protocols and Good Operating Practices in the Study of Cannabinoid Receptors3. Real-Time Measurement of Cannabinoid Receptor-Mediated cAMP Signaling4. Techniques for the Cellular and Subcellular Localization of Endocannabinoid Receptors and Enzymes in the Mammalian Brain5. Endocannabinoid Transport Proteins: Discovery of Tools to Study Sterol Carrier Protein-26. Lipidomics: A Corrective Lens for Enzyme Myopia7. Functional Analysis of Mitochondrial CB1 Cannabinoid Receptors (mtCB1) in the Brain8. Modeling Neurodegenerative Disorders for Developing Cannabinoid-Based Neuroprotective Therapies9. Metabolic Profiling of CB1 Neutral Antagonists10. Ligand-Assisted Protein Structure (LAPS): An Experimental Paradigm for Characterizing Cannabinoid-Receptor Ligand-Binding Domains11. New Methods for the Synthesis of Cannabidiol Derivatives12. Approaches to Assess Biased Signaling at the CB1R Receptor13. Design and Synthesis of Cannabinoid 1 Receptor (CB1R) Allosteric Modulators: Drug Discovery Applications14. Assessing Allosteric Modulation of CB1 at the Receptor and Cellular Levels15. Purification of Functional CB1 and Analysis by Site-Directed Fluorescence Labeling Methods16. Mass Spectrometry Analysis of Human CB2 Cannabinoid Receptor and Its Associated Proteins17. Expression and NMR Structural Studies of Isotopically Labeled Cannabinoid Receptor Type II18. Methods for the Development of In Silico GPCR Models19. Molecular Dynamics Methodologies for Probing Cannabinoid Ligand/Receptor Interaction