The Zebrafish in Biomedical Research

Samuel Cartner, D.V. M., Ph.D., is the Assistant Vice President for Animal Research Services and Director of the Animal Resources Program (ARP) at the University of Alabama at Birmingham (UAB). He received his DVM from Auburn College of Veterinary Medicine and PhD from UAB. Dr. Cartner has served in multiple roles with organizations that promote premium laboratory animal care. Dr. Cartner is a faculty member in the Department of Genetics. His research interest includes genetic susceptibility to infectious disease, and the development of animal models of human and animal disease. Recently Dr. Cartner has focused on investigations that lead to improvements of laboratory animal care and use. Judith S. Eisen, Ph.D., earned her Doctorate at Brandeis University. She is a professor at the University of Oregon and a member of the Institute of Neuroscience at Oregon. The focus of Eisen’s research is to understand the mechanisms underlying development and function of the vertebrate nervous system. Eisen uses zebrafish in her research. Susan C. Farmer, DVM, Ph.D., is the Senior Clinical Veterinarian and Co-Director of the Zebrafish Research Facility at the University of Alabama. Services offered by the facility are husbandry, larviculture, cryopreservation and research support. Dr. Farmer have her DVM degree from Mississippi State University and her Ph.D. in Pathology/Experimental Pathology from the University of Alabama at Birmingham. Karen Guillemin, Ph.D., earned her Doctorate at Stanford University. She is a Professor at the University of Oregon and a member of the Institute of Molecular Biology at Oregon. Dr. Guillemin studies host-microbe interactions in development and disease, and uses zebrafish in her research. Michael Kent, Ph.D., is a Professor of Microbiology at Oregon State University in the College of Veterinary Medicine. Kent’s lab focuses on two major research areas: diseases of zebrafish in research facilities and impacts of pathogens on wild salmonid fishes. Dr. Kent is also a co-PI at the Zebrafish International Resource Center where he assists with health studies and their diagnostic service. George E. Sanders, DVM, M.S., is certified as a Fish Pathologist by the American Fisheries Society Fish Health Section, a Senior Lecturer, and the Aquatic Animal Program Director for the Department of Comparative Medicine at the University of Washington. He provides multiple resources, program development, facility and systems design, training, instruction, regulatory compliance, and coordinates veterinary care and pathology for all aquatic research animals. Dr. Sanders received his DVM from Louisiana State University School of Veterinary Medicine and his Masters of Science in Comparative Medicine from the Department of Comparative Medicine at the University of Washington.

• I.  Introduction1. History of Zebrafish Research2. Taxonomy/Phylogeny3.  Zebrafish Genetics4.  Geographic Range and Natural Distribution5.  Behavior of Wild PopulationsII.  BiologyAppearance, Development, Anatomy, Physiology6.  Zebrafish in Biomedical Research: Head and Body: Anatomy7.  Establishing the Body Plan: The First 24 Hours of Zebrafish DevelopmentOrgan Systems: Development, Anatomy, Physiology8.  Zebrafish Integumentary System9.  Zebrafish Pigmentation10. Respiratory System11. Skeletal System Morphophysiology12. Zebrafish Myology13. It Takes Guts: Development of the Embryonic and Juvenile Zebrafish Digestive System14. The Zebrafish Cardiovascular System15. Development of the Zebrafish Pronephric and Mesonephric Kidneys16. Reproductive System17. Endocrine Systems18. Nervous Systems19. Immunology20. Physiology: Hematology and Clinical Chemistry, Gas Exchange, and Regulatory OsmolalityTissues and Organs Associated with Special Senses21. Zebrafish in Biomedical Research: The Retina and Vision22. The Mechanosensory Lateral Line System23. Inner Ear and HearingHusbandry24. Introduction to Zebrafish Husbandry25. Aquatic Facility Design Considerations: Incorporating Aquatics into an Animal Facility26. Aquatic Housing27. Cleaning and Disinfection of Life Systems28. Zebrafish Aquatic Systems: Preventative Maintenance and Troubleshooting29. Water Quality for Zebrafish Cultura30. Recirculating Aquaculture Systems (RAS) for Zebrafish Culture31. Zebrafish Breeding and Colony Management32. Zebrafish Larviculture33. Zebrafish Nutrition--Moving Forward34. Anesthesia, Analgesia, and Euthanasia of the Laboratory Zebrafish35. Health Xurveillance Programs36. Importation and Quarantine37. Export and Transportation of Zebrafish38. Regulations, Policies and Guidelines Pertaining to the Use of Zebrafish in Biomedical ResearchIII. Diseases39. Water Quality and Idiopathic Diseases of Laboratory Zebrafish40. Important Parasites of Zebrafish in Research Facilities41. Bacterial and Fungal Diseases of Zebrafish42. Viral Diseases43. Nonexperimentally Induced Neoplastic and Proliferative Lesions in Laboratory Zebrafish44. Special Procedures for Zebrafish Diagnostics45. Zebrafish as a Model to Understand Vertebrate Development46. Zebrafish as a Model for Revealing the Neuronal Basis of Behavior47. Zebrafish as a Model to Understand Human Genetic Diseases48. Zebrafiish as a Model for Investigating49. Targeted Editing of Zebrafish Genes to Understand Gene Function and Human Disease Pathology50. Zebrafish as a Platform for Genetic Screening51. Zebrafish as a Platform for Drug Screening