Ubiquitin and Ubiquitin-like Protein Modifiers

Dr. Mark W Hochstrasser is a Eugene Higgins Professor of Molecular Biophysics and Biochemistry and Professor of Molecular, Cellular, and Developmental BiologyYale University, USA.

Praise for the Series:"Should be on the shelves of all libraries in the world as a whole collection." --CHEMISTRY IN INDUSTRY"The work most often consulted in the lab." --ENZYMOLOGIA"The Methods in Enzymology series represents the gold-standard." --NEUROSCIENCE

• 1. Semisynthesis of ubiquitinated histone H2B with a native or nonhydrolyzable linkageMichael Morgan, Muhammad Jbara, Ashraf Brik and Cynthia Wolberger2. Dual-color pulse-chase ubiquitination assays to simultaneously monitor substrate priming and extensionDaniel C. Scott and Brenda A. Schulman3. A genetic approach to study polyubiquitination in Saccharomyces cerevisiaeFernando Meza-Gutierrez, Deniz Simsek and David P. Toczyski4. Enzymatic preparation of monoubiquitinated FANCD2 and FANCI proteinsViduth K. Chaugule, Connor Arkinson, Rachel Toth and Helen Walden5. Methods to measure ubiquitin chain length and linkageFumiaki Ohtake, Hikaru Tsuchiya, Keiji Tanaka and Yasushi Saeki6. Detection of ubiquitination activity and identification of ubiquitinated substrates using TR-TUBEYukiko Yoshida, Yasushi Saeki, Hikaru Tsuchiya and Keiji Tanaka7. Methods to study phosphoribosylated ubiquitin ligation and removalJiazhang Qiu and Zhao-Qing Luo8. Biochemical characterization of SUMO-conjugating enzymes by in vitro sumoylation assaysNathalie Eisenhardt, Dragana Ilic, Easa Nagamalleswari and Andrea Pichler9. Methods to study SUMO dynamics in yeastStefan Pabst, Lennard-Maximilian Döring, Natasha Petreska and R. Jürgen Dohmen10. Approaches for investigating the extracellular signaling function of ISG15Caleb D. Swaim, Larissa A. Canadeo and Jon M. Huibregtse11. Analysis of modification and proteolytic targeting by the ubiquitin-like modifier FAT10Annette Aichem, Annika N. Boehm, Nicola Catone, Gunter Schmidtke and Marcus Groettrup12. Methods to analyze STUbL activityEmma Branigan, Anna Plechanovová and Ronald T. Hay13. Quantitative analysis of USP activity in vitroShreya Dharadhar, Robbert Q. Kim, Michael Uckelmann and Titia K. Sixma14. Evaluating enzyme activities and structures of DUBsJonathan N. Pruneda and David Komander15. Purification and functional characterization of the DUB domain of SdeAKedar Puvar, Shalini Iyer, Michael J. Sheedlo and Chittaranjan Das16. Profiling DUBs and Ubl-specific proteases with activity-based probesPaul P. Geurink, Gerbrand J. van der Heden van Noort, Monique P.C. Mulder, Robert C.M. Knaap, Marjolein Kikkert and Huib Ovaa17. Assays of SUMO protease/isopeptidase activity and function in mammalian cells and tissuesKathrin Kunz, Stefan Müller and Luca Mendler