CRISPR-Cas Enzymes

Scott Bailey is at Johns Hopkins Bloomberg School of Public Health, USA

Praise for the Series:"Should be on the shelves of all libraries in the world as a whole collection." --CHEMISTRY IN INDUSTRY"The work most often consulted in the lab." --ENZYMOLOGIA"The Methods in Enzymology series represents the gold-standard." --NEUROSCIENCE

• 1. Predicting and visualizing features of CRISPR–Cas systemsMatthew A. Nethery and Rodolphe Barrangou2. Reconstitution and biochemical characterization of ribonucleoprotein complexes in Type I-E CRISPR–Cas systemsYibei Xiao and Ailong Ke3. Sortase-mediated fluorescent labeling of CRISPR complexesKaylee E. Dillard, Jeffrey M. Schaub, Maxwell W. Brown, Fatema A. Saifuddin, Yibei Xiao, Erik Hernandez, Samuel D. Dahlhauser, Eric V. Anslyn, Ailong Ke and Ilya J. Finkelstein4. Fluorescence-based methods for measuring target interference by CRISPR–Cas systemsPhong T. Phan, Michael Schelling, Chaoyou Xue and Dipali G. Sashital5. Probing Cascade complex composition and stability using native mass spectrometry techniquesAngela Patterson, Monika Tokmina-Lukaszewska and Brian Bothner6. High-throughput determination of in vivo DNA sequence preferences for Cas protein binding using Library-ChIPJoseph T. Wade7. Live-cell single-particle tracking photoactivated localization microscopy of Cascade-mediated DNA surveillanceBartosz Turkowyd, Hanna Müller-Esparza, Vanessa Climenti, Niklas Steube, Ulrike Endesfelder and Lennart Randau8. In vitro assembly of thermostable Csm complex in CRISPR–Cas type III/A systemKwang-Hyun Park, Yan An and Eui-Jeon Woo9. Investigation of the cyclic oligoadenylate signaling pathway of type III CRISPR systemsChristophe Rouillon, Januka S. Athukoralage, Shirley Graham, Sabine Grüschow and Malcolm F. White10. A pipeline for characterization of novel Cas9 orthologsTautvydas Karvelis, Joshua K. Young and Virginijus Siksnys11. Preparation and electroporation of Cas12a/Cpf1-guide RNA complexes for introducing large gene deletions in mouse embryonic stem cellsLucas Kissling, Asun Monfort, Daan C. Swarts, Anton Wutz and Martin Jinek12. Directed evolution studies of a thermophilic Type II-C Cas9Travis H. Hand, Anuska Das and Hong Li13. Kinetic characterization of Cas9 enzymesMu-Sen Liu, Shanzhong Gong, Helen-Hong Yu, David W. Taylor and Kenneth A. Johnson14. Single-molecule FRET studies of Cas9 endonucleaseViktorija Globyte and Chirlmin Joo15. CRISPR–Cas molecular beacons as tool for studies of assembly of CRISPR–Cas effector complexes and their interactions with DNAVladimir Mekler, Konstantin Kuznedelov, Leonid Minakhin, Karthik Murugan, Dipali G. Sashital and Konstantin Severinov16. Adapting dCas9-APEX2 for subnuclear proteomic profilingXin D. Gao, Tomás C. Rodríguez and Erik J. Sontheimer17. CRISPR–Cas10 assisted editing of virulent staphylococcal phagesS.M. Nayeemul Bari and Asma Hatoum-Aslan18. Reconstitution of CRISPR adaptation in vitro and its detection by PCRRobert D. Fagerlund, Timothy J. Ferguson, Howard W.R. Maxwell, Helen K. Opel-Reading, Kurt L. Krause and Peter C. Fineran