Three-Dimensional Electron Microscopy

Thomas Müller-Reichert is a Professor of Structural Cell Biology at the Technische Universität Dresden (TU Dresden, Germany). He is interested in how the microtubule cytoskeleton is modulated within cells to fulfill functions in mitosis, meiosis and abscission. The Müller-Reichert lab is mainly applying correlative light microscopy and electron tomography to study the 3D organization of microtubules in early embryos and meiocytes of the nematode Caenorhabditis elegans, and also in mammalian cells in culture. He has published over 75 papers and edited several volumes of the Methods in Cell Biology series on electron microscopy and CLEM.TMR obtained his PhD at the Swiss Federal Institute of Technology (ETH) in Zurich and moved afterwards for a post-doc to the EMBL in Heidelberg (Germany). He was a visiting scientist with Dr. Kent McDonald (UC Berkeley, USA). Together with Paul Verkade, he set up the electron microscope facility at the newly founded Max Planck Institute of Molecular Cell Biology and Genetics (MPI-CBG). Since 2010 he is a scientific group leader and head of the Core Facility Cellular Imaging (CFCI) of the Faculty of Medicine Carl Gustav Carus of the TU Dresden. He acted as president of the German Society for Electron Microscopy (Deutsche Gesellschaft für Elektronenmikroskopie, DGE) from 2018 to 2019.He taught numerous courses and workshops on high-pressure freezing and Correlative Light and Electron Microscopy. Dr. Pigino works at Max Planck Institute of Molecular Cell Biology and Genetics, Dresden, Germany

• 1. FIB-SEM of mouse nervous tissue: Fast and slow sample preparationAnna M. Steyer, Andreas Schertel, Christos Nardis and Wiebke Möbius2. Expedited large-volume 3-D SEM workflows for comparative microanatomical imagingGerald John Shami, Delfine Cheng and Filip Braet3. Serial-section electron microscopy using automated tape-collecting ultramicrotome (ATUM)Valentina Baena, Richard Lee Schalek, Jeff William Lichtman and Mark Terasaki4. Serial block face-scanning electron microscopy for volume electron microscopySaskia Lippens, Anna Kremer, Peter Borghgraef and Chris Guérin5. Combining serial block face and focused ion beam scanning electron microscopy for 3D studies of rare eventsChristopher J. Guérin, Anna Kremer, Peter Borghgraef, Andy Y. Shih and Saskia Lippens6. Yeast membraneless compartments revealed by correlative light microscopy and electron tomographyGuendalina Marini and Gaia Pigino7. In situ analysis of male meiosis in C. elegansGunar Fabig, Anna Schwarz, Cynthia Striese, Michael Laue and Thomas Müller-Reichert8. Software for automated acquisition of electron tomography tilt seriesGuenter P. Resch9. In situ cryo-electron tomography and subtomogram averaging of intraflagellar transport trainsMareike Jordan and Gaia Pigino10. CryoSTEM tomography in biologySharon G. Wolf and Michael Elbaum11. Subtomogram averaging from cryo-electron tomogramsKendra E. Leigh, Paula P. Navarro, Stefano Scaramuzza, Wenbo Chen, Yingyi Zhang, Daniel Castaño-Díez and Misha Kudryashev12. Computational methods for stitching, alignment, and artifact correction of serial section dataStephan Saalfeld13. Content-aware image restoration for electron microscopyTim-Oliver Buchholz, Alexander Krull, Réza Shahidi, Gaia Pigino, Gáspár Jékely and Florian Jug