Jungsan Sohn is at Johns Hopkins School of Medicine, MD, USA

• 1. Intercellular communication in the innate immune system through the cGAS-STING pathwayRamya Nandakumar, Samuel Joseph Windross and Søren R. Paludan2. Analysis of human cGAS activity and structureWen Zhou, Aaron T. Whiteley and Philip J. Kranzusch3. Chemical synthesis, purification, and characterization of 3′-5′-linked canonical cyclic dinucleotides (CDNs)Changhao Wang, Min Hao, Qianqian Qi, Yashao Chen and Jörg S. Hartig4. An SPR-based analysis of cGAS substrate KD and steady-state KM valuesErik C. Ralph and Justin Hall5. A pyrophosphatase-coupled assay to monitor the NTase activity of cGASRichard Hooy and Jungsan Sohn6. Tracking the polymerization of DNA sensors and inflammasomes using FRETZachary Mazanek and Jungsan Sohn7. Monitoring gasdermin pore formation in vitroShiyu Xia, Jianbin Ruan and Hao Wu8. Measuring TREX1 and TREX2 exonuclease activitiesWayne O. Hemphill and Fred W. Perrino9. Kinetics of DNA: Protein association and dissociation by stopped-flow spectroscopyJohannes Rudolph and Karolin Luger10. Characterization of DNA bound cyclic GMP-AMP synthase using atomic force microscopy imagingAlexander Lushnikov, Richard Hooy, Jungsan Sohn and Alexey Krasnoslobodtsev11. Preparation of filamentous proteins for electron microscopy visualization and reconstructionMariusz Matyszewski and Jungsan Sohn12. Cryo-EM studies of NAIP–NLRC4 inflammasomesNicole Haloupek, Patricia Grob, Jeannette Tenthorey, Russell E. Vance and Eva Nogales13. An integrative protocol for the structure determination of the mouse ASC-PYD filamentAdam Mazur, Petr Broz and Sebastian Hiller14. Protein interactions of the inflammasome adapter ASC by solution NMRPedro Diaz-Parga and Eva de Alba15. Tracking DNA sensor and inflammasome complexes in cells using confocal microscopyBrendan Antiochos16. Assessment of inflammasome and type I IFN responses to DNA viruses and DNA PAMPSGuido Massaccesi and Michael A. Chattergoon17. Biochemical and microscopic analysis of inflammasome complex formationPatrycja Swacha, Nelson Ong’ondo Gekara and Saskia F. Erttmann18. Comet and micronucleus assays for analyzing DNA damage and genome integrityHui Jiang and Nelson Ong'ondo Gekara19. Workflows and considerations for investigating protein interactions of viral DNA sensorsTimothy R. Howard, Bokai Song and Ileana M. Cristea20. TUBE and UbiCRest assays for elucidating polyubiquitin modifications in protein complexesSwarupa Panda and Nelson Ong'ondo Gekara

Jungsan Sohn is at Johns Hopkins School of Medicine, MD, USA

• 1. Intercellular communication in the innate immune system through the cGAS-STING pathwayRamya Nandakumar, Samuel Joseph Windross and Søren R. Paludan2. Analysis of human cGAS activity and structureWen Zhou, Aaron T. Whiteley and Philip J. Kranzusch3. Chemical synthesis, purification, and characterization of 3′-5′-linked canonical cyclic dinucleotides (CDNs)Changhao Wang, Min Hao, Qianqian Qi, Yashao Chen and Jörg S. Hartig4. An SPR-based analysis of cGAS substrate KD and steady-state KM valuesErik C. Ralph and Justin Hall5. A pyrophosphatase-coupled assay to monitor the NTase activity of cGASRichard Hooy and Jungsan Sohn6. Tracking the polymerization of DNA sensors and inflammasomes using FRETZachary Mazanek and Jungsan Sohn7. Monitoring gasdermin pore formation in vitroShiyu Xia, Jianbin Ruan and Hao Wu8. Measuring TREX1 and TREX2 exonuclease activitiesWayne O. Hemphill and Fred W. Perrino9. Kinetics of DNA: Protein association and dissociation by stopped-flow spectroscopyJohannes Rudolph and Karolin Luger10. Characterization of DNA bound cyclic GMP-AMP synthase using atomic force microscopy imagingAlexander Lushnikov, Richard Hooy, Jungsan Sohn and Alexey Krasnoslobodtsev11. Preparation of filamentous proteins for electron microscopy visualization and reconstructionMariusz Matyszewski and Jungsan Sohn12. Cryo-EM studies of NAIP–NLRC4 inflammasomesNicole Haloupek, Patricia Grob, Jeannette Tenthorey, Russell E. Vance and Eva Nogales13. An integrative protocol for the structure determination of the mouse ASC-PYD filamentAdam Mazur, Petr Broz and Sebastian Hiller14. Protein interactions of the inflammasome adapter ASC by solution NMRPedro Diaz-Parga and Eva de Alba15. Tracking DNA sensor and inflammasome complexes in cells using confocal microscopyBrendan Antiochos16. Assessment of inflammasome and type I IFN responses to DNA viruses and DNA PAMPSGuido Massaccesi and Michael A. Chattergoon17. Biochemical and microscopic analysis of inflammasome complex formationPatrycja Swacha, Nelson Ong’ondo Gekara and Saskia F. Erttmann18. Comet and micronucleus assays for analyzing DNA damage and genome integrityHui Jiang and Nelson Ong'ondo Gekara19. Workflows and considerations for investigating protein interactions of viral DNA sensorsTimothy R. Howard, Bokai Song and Ileana M. Cristea20. TUBE and UbiCRest assays for elucidating polyubiquitin modifications in protein complexesSwarupa Panda and Nelson Ong'ondo Gekara

Jungsan Sohn is at Johns Hopkins School of Medicine, MD, USA

• 1. Intercellular communication in the innate immune system through the cGAS-STING pathwayRamya Nandakumar, Samuel Joseph Windross and Søren R. Paludan2. Analysis of human cGAS activity and structureWen Zhou, Aaron T. Whiteley and Philip J. Kranzusch3. Chemical synthesis, purification, and characterization of 3′-5′-linked canonical cyclic dinucleotides (CDNs)Changhao Wang, Min Hao, Qianqian Qi, Yashao Chen and Jörg S. Hartig4. An SPR-based analysis of cGAS substrate KD and steady-state KM valuesErik C. Ralph and Justin Hall5. A pyrophosphatase-coupled assay to monitor the NTase activity of cGASRichard Hooy and Jungsan Sohn6. Tracking the polymerization of DNA sensors and inflammasomes using FRETZachary Mazanek and Jungsan Sohn7. Monitoring gasdermin pore formation in vitroShiyu Xia, Jianbin Ruan and Hao Wu8. Measuring TREX1 and TREX2 exonuclease activitiesWayne O. Hemphill and Fred W. Perrino9. Kinetics of DNA: Protein association and dissociation by stopped-flow spectroscopyJohannes Rudolph and Karolin Luger10. Characterization of DNA bound cyclic GMP-AMP synthase using atomic force microscopy imagingAlexander Lushnikov, Richard Hooy, Jungsan Sohn and Alexey Krasnoslobodtsev11. Preparation of filamentous proteins for electron microscopy visualization and reconstructionMariusz Matyszewski and Jungsan Sohn12. Cryo-EM studies of NAIP–NLRC4 inflammasomesNicole Haloupek, Patricia Grob, Jeannette Tenthorey, Russell E. Vance and Eva Nogales13. An integrative protocol for the structure determination of the mouse ASC-PYD filamentAdam Mazur, Petr Broz and Sebastian Hiller14. Protein interactions of the inflammasome adapter ASC by solution NMRPedro Diaz-Parga and Eva de Alba15. Tracking DNA sensor and inflammasome complexes in cells using confocal microscopyBrendan Antiochos16. Assessment of inflammasome and type I IFN responses to DNA viruses and DNA PAMPSGuido Massaccesi and Michael A. Chattergoon17. Biochemical and microscopic analysis of inflammasome complex formationPatrycja Swacha, Nelson Ong’ondo Gekara and Saskia F. Erttmann18. Comet and micronucleus assays for analyzing DNA damage and genome integrityHui Jiang and Nelson Ong'ondo Gekara19. Workflows and considerations for investigating protein interactions of viral DNA sensorsTimothy R. Howard, Bokai Song and Ileana M. Cristea20. TUBE and UbiCRest assays for elucidating polyubiquitin modifications in protein complexesSwarupa Panda and Nelson Ong'ondo Gekara