Proteomic and Metabolomic Approaches to Biomarker Discovery

Haleem J. Issaq received his B.S. degree from the American College of Istanbul, Turkey, a M.S. degree from the Technion, Israel Institute of Technology, and a Ph.D. in analytical chemistry from Georgetown University in 1972. He was a Principal Scientist and Head of the Separation Technologies and Assay Development Group within the Laboratory of Proteomics and Analytical Technologies, SAIC-Frederick, Inc., located within the National Cancer Institute at Frederick, Maryland. His research interests include multidimensional separation of complex protein/peptide mixtures using liquid-phase-based separations (LC/LC, IEF/LC, and LC/CE), fast separation of estrogens, and use of HPLC/MS metabolomics for disease biomarker detection. He is the author or coauthor of over 350 scientific publications and the editor of A Century of Separation Science. He is the recipient of numerous awards, including Maryland Chemist of the Year. Dr. Timothy Veenstra obtained his PhD in Biochemistry from University of Windsor (Windsor, ON) and completed a three-year post-doctoral fellowship at the Mayo Clinic under the guidance of Dr. Rajiv Kumar. He has over 20 years of research experience both in academics and as a Senior Vice President for a biotech company that focused on neurological diseases. With more than 380 published works (including 2 books), and eight patents, Dr. Timothy Veenstra brings a wealth of knowledge and experience to share with Maranatha’s science students.

• 1. Biomarker discovery: Study design and execution2. Proteomic and mass spectrometry technologies for biomarker discovery3. Tissue sample preparation for proteomic analysis4. Sample preparation in global metabolomics of biological fluids and tissues5. Serum and plasma collection: Preanalytical variables and standard operating procedures in biomarker research6. Sample depletion, fractionation, and enrichment for biomarker discovery7. Current NMR strategies for biomarker8. Gas chromatography/mass spectrometrybased metabonomics9. Liquid chromatographic methods combined with mass spectrometry in metabolomics10. Capillary electrophoresis-mass spectrometry for proteomic and metabolic11. Associating 2-DE and CPLLs for low-abundance protein discovery: A winning strategy12. Two-dimensional difference in gel electrophoresis for biomarker discovery 13. Affinity-targeting schemes for protein biomarkers14. Protein and metabolite identification15. Quantitative proteomics in development of disease protein biomarkers16. Mass spectrometry and NMR spectroscopy based quantitative metabolomics17. Top-down mass spectrometry for protein molecular diagnostics, structure analysis, and biomarker discovery18. Using data-independent mass spectrometry to extend detectable dynamic range without prior fractionation19. Imaging mass spectrometry of intact biomolecules in tissue section20. Mass spectrometry-based approach for protein biomarker verification21. Mass spectrometry metabolomic data handling for biomarker discovery22. Analytical methods and biomarker validation23. Multivariate analysis for metabolomics and proteomics data24. Cell surface protein enrichment for biomarker and drug target discovery using mass spectrometry-based proteomic25. Advances in lipidomics for cancer biomarker discovery26. Mass spectrometry for the identification of protein biomarkers in urinary extracellular vesicles27. Designing clinical studies for biomarker discovery: The DESIGN criteria