Post-translational Modifications That Modulate Enzyme Activity

Benjamin A. Garcia, Ph.D., Presidential Professor, Professor of Biochemistry and Biophysics, Director of Quantitative Proteomics, Penn Epigenetics Institute, Department of Biochemistry and Biophysics, Smilow Center for Translational Research, University of Pennsylvania School of Medicine

• 1. Crosstalk between cellular metabolism and histone acetylationSophie Trefely, Mary T. Doan and Nathaniel W. Snyder2. Purification and enzymatic assay of class I histone deacetylase enzymesMark K. Adams, Charles A.S. Banks, Sayem Miah, Maxime Killer and Michael P. Washburn3. Multiplexed quantitative phosphoproteomics of cell line and tissue samplesJohannes Kreuzer, Amanda Edwards and Wilhelm Haas4. Differentiation of peptide isomers and epimers by radical-directed dissociationTyler R. Lambeth and Ryan R. Julian5. Biochemical analysis of protein arginylationJunling Wang, John R. Yates and Anna Kashina6. Site-specific determination of lysine acetylation stoichiometries on the proteome-scaleYue Chen and Yunan Li7. RNA modifications and the link to human diseaseAmber Yanas and Kathy Fange Liu8. Isolation and characterization of glycosylated neuropeptidesYang Liu, Qinjingwen Cao and Lingjun Li9. Utilizing intein trans-splicing for in vivo generation of site-specifically modified proteinsIgor Maksimovic, Devin Ray, Qingfei Zheng and Yael David10. Systematic and site-specific analysis of N-glycoproteins on the cell surface by integrating bioorthogonal chemistry and MS-based proteomicsFangxu Sun and Ronghu Wu11. Silencing glycosaminoglycan functions in mouse embryonic stem cells with small molecule antagonistsSourav Chatterjee, Tesia N. Stephenson, Austen L. Michalak, Kamil Godula and Mia L. Huang12. Biochemical and structural analysis of N-terminal acetyltransferasesLeah Gottlieb and Ronen Marmorstein13. Site-specific analysis of the Asp- and Glu-ADP-ribosylated proteome by quantitative mass spectrometryPeng Li, Yuanli Zhen and Yonghao Yu14. Analysis of the topology of ubiquitin chainsLucia Geis-Asteggiante, Amanda E. Lee and Catherine Fenselau15. Analysis of cardiac troponin proteoforms by top-down mass spectrometryTimothy N. Tiambeng, Trisha Tucholski, Zhijie Wu, Yanlong Zhu, Stanford D. Mitchell, David S. Roberts, Yutong Jin and Ying Ge16. Assays for tyrosine phosphorylation in human cellsMonica Kruk, Naomi Widstrom, Sampreeti Jena, Nicole L. Wolter, John F. Blankenhorn, Ibrahim Abdalla, Tzu-Yi Yang and Laurie L. Parker17. Metabolomics analysis of lipid metabolizing enzyme activityTimothy B. Ware, Myungsun Shin and Ku-Lung Hsu18. Simplified high yield TAILS terminomics using a new HPG-ALD 800K-2000 polymer with precipitationNestor Solis, Anilkumar Parambath, Srinivas Abbina, Jayachandran Kizhakkedathu and Christopher M. Overall19. Methods for the expression, purification, and crystallization of histone deacetylase 6–inhibitor complexesJeremy D. Osko and David W. Christianson20. Quantitative analysis of global protein lysine methylation by mass spectrometryPeder J. Lund, Stephanie M. Lehman and Benjamin A. Garcia21. The roles of S-nitrosylation and S-glutathionylation in Alzheimer’s diseaseRyan R. Dyer, Katarena I. Ford and Renã A. S. Robinson22. Expression of authentic post-translationally modified proteins in organisms with expanded genetic codesKyle Mohler and Jesse Rinehart23. Preparation of a new construct of human histone deacetylase 8 for the crystallization of enzyme-inhibitor complexesNicholas J. Porter and David W. Christianson24. Methods for characterizing protein acetylation during viral infectionLaura A. Murray, Ashton N. Combs, Pranav Rekapalli and Ileana M. Cristea