Tumor Immunology and Immunotherapy - Molecular Methods

Lorenzo Galluzzi is Assistant Professor of Cell Biology in Radiation Oncology at the Department of Radiation Oncology of the Weill Cornell Medical College, Honorary Assistant Professor Adjunct with the Department of Dermatology of the Yale School of Medicine, Honorary Associate Professor with the Faculty of Medicine of the University of Paris, and Faculty Member with the Graduate School of Biomedical Sciences and Biotechnology of the University of Ferrara, the Graduate School of Pharmacological Sciences of the University of Padova, and the Graduate School of Network Oncology and Precision Medicine of the University of Rome “La Sapienza”. Moreover, he is Associate Director of the European Academy for Tumor Immunology and Founding Member of the European Research Institute for Integrated Cellular Pathology.Galluzzi is best known for major experimental and conceptual contributions to the fields of cell death, autophagy, tumor metabolism and tumor immunology. He has published over 450 articles in international peer-reviewed journals and is the Editor-in-Chief of four journals:OncoImmunology (which he co-founded in 2011), International Review of Cell and Molecular Biology, Methods in Cell biology, and Molecular and Cellular Oncology (which he co-founded in 2013). Additionally, he serves as Founding Editor for Microbial Cell and Cell Stress, and Associate Editor for Cell Death and Disease, Pharmacological Research and iScience. Nils-Petter Rudqvist received his M.Sc. (Physics, 2009) and Ph.D. (Medical Science, 2015) from the University of Gothenburg, Sweden. He decided to pursue an academic career and continue with his postdoctoral training in US. He first joined the Center for Radiological Research at Columbia University in New York where he studied gene signatures of radiation exposure. He then moved to Weill Cornell Medicine to join the program in radiation and immunity under the mentorship of Dr. Demaria. His current research is focused on investigating which neoantigens are key targets of the radiation-induced anti-tumor T cell response in mice and in patients treated with radiotherapy and immune checkpoint blockade. He recently demonstrated in a mouse model that radiation therapy diversifies the TCR repertoire of tumor infiltrating lymphocytes, an effect crucial for its synergy with immune checkpoint blockade treatment. Nils-Petter has also defined unique patters of expansion of TCR clonotypes in patients who respond or not to treatment with radiotherapy and ipilimumab. He has published 20 articles in peer-reviewed scientific journals and 60+ scientific conference abstracts.

• 1. Detection of circulating tumor DNA (ctDNA) by digital droplet polymerase chain reaction (dd-PCR) in liquid biopsiesSara Valpione and Luca Campana2. Detection and quantification of cytosolic DNASheila Spada, Takahiro Yamazaki and Claire Vanpouille-Box3. Methods to detect endogenous dsRNA induction and recognitionIlias Ettayebi, Helen Loo Yau and Daniel D. De Carvalho4. Quantification of eIF2alpha phosphorylation during immunogenic cell deathLucillia Bezu, Alejandra Wu Chuang, Juliette Humeau, Guido Kroemer and Oliver Kepp5. Assessment of annexin A1 release during immunogenic cell deathElisa Elena Baracco, Adriana Petrazzuolo and Guido Kroemer6. Luciferase-assisted detection of extracellular ATP and ATP metabolites during immunogenic death of cancer cellsGeorge R. Dubyak7. Quinacrine-mediated detection of intracellular ATPSabrina Forveille, Juliette Humeau, Allan Sauvat, Lucillia Bezu, Guido Kroemer and Oliver Kepp8. Structure, function and techniques of investigation of the P2X7 receptor (P2X7R) in mammalian cellsFrancesco Di Virgilio, Lin-Hua Jiang, Sèbastien Roger, Simonetta Falzoni, Alba Clara Sarti, Valentina Vultaggio-Poma, Paola Chiozzi and Elena Adinolfi9. LEGENDplex™: Bead-assisted multiplex cytokine profiling by flow cytometryJason S. Lehmann, Priyanka Rughwani, Melissa Kolenovic, Shaoquan Ji and Binggang Sun10. Methods for measuring HMGB1 release during immunogenic cell deathLiwei Zhao, Peng Liu, Oliver Kepp and Guido Kroemer11. Different measures of HMGB1 location in cancer immunologyCarolina Mendonça Gorgulho, Pranav Murthy, Lance Liotta, Virginia Espina and Michael T. Lotze12. Reliable detection of indoleamine 2,3 dioxygenase-1 in murine cells and tissuesSunil Thomas, Lisa Laury-Kleintop and George C. Prendergast13. Quantification of IDO1 enzyme activity in normal and malignant tissuesLijie Zhai, Erik Ladomersky, April Bell, Corey Dussold, Krislyn Cardoza, Jun Qian, Kristen L. Lauing and Derek A. Wainwright14. Enzyme activity of circulating CD73 in human serumSilvana Morello, Roberta Turiello, Gabriele Madonna, Aldo Pinto, Paolo A. Ascierto andMariaelena Capone15. Measurement of CD73 enzymatic activity using luminescence-based and colorimetric assaysBertrand Allard, Isabelle Cousineau, Kathleen Spring and John Stagg16. Distinguishing perforin-mediated lysis and granzyme-dependent apoptosisJesse A. Rudd-Schmidt, Joseph A. Trapani and Ilia Voskoboinik17. Elucidating the role of an immunomodulatory protein in cancer: From protein expression to functional characterizationLeepakshi Khurana, Mei ElGindi, Pathricia V. Tilstam and Georgios Pantouris18. In vitro assay for the development of small molecule inhibitors targeting PD-1/PD-L1Wenjie Zhai, Xiuman Zhou, Jiangfeng Du and Yanfeng Gao19. Crystallographic approaches to study the interaction modes of PD-1- and CTLA-4-blocking antibodiesNorimichi Nomura, Yayoi Nomura, Yumi Sato and So Iwata20. High-throughput discovery of cancer-targeting TCRsLukas Bunse, Edward W. Green and Michael Platten21. TCR validation toward gene therapy for cancerEdward W. Green, Lukas Bunse, Matthias Bozza, Khwab Sanghvi and Michael Platten22. Analysis of TCR β CDR3 sequencing data for tracking anti-tumor immunityJiajia Zhang, Zhicheng Ji and Kellie N. Smith23. Quantitative analysis of the T cell receptor repertoireImran Uddin, Annemarie Woolston, Thomas Peacock, Kroopa Joshi, Mazlina Ismail, Tahel Ronel, Connor Husovsky and Benny Chain24. CRATES: A one-step assembly method for Class 2 CRISPR arraysChunyu Liao, Rebecca A. Slotkowski and Chase L. Beisel