Tumor Immunology and Immunotherapy - Cellular Methods Part A

Lorenzo Galluzzi is Assistant Professor of Cell Biology in Radiation Oncology at the Department of Radiation Oncology of the Weill Cornell Medical College, Honorary Assistant Professor Adjunct with the Department of Dermatology of the Yale School of Medicine, Honorary Associate Professor with the Faculty of Medicine of the University of Paris, and Faculty Member with the Graduate School of Biomedical Sciences and Biotechnology of the University of Ferrara, the Graduate School of Pharmacological Sciences of the University of Padova, and the Graduate School of Network Oncology and Precision Medicine of the University of Rome “La Sapienza”. Moreover, he is Associate Director of the European Academy for Tumor Immunology and Founding Member of the European Research Institute for Integrated Cellular Pathology.Galluzzi is best known for major experimental and conceptual contributions to the fields of cell death, autophagy, tumor metabolism and tumor immunology. He has published over 450 articles in international peer-reviewed journals and is the Editor-in-Chief of four journals:OncoImmunology (which he co-founded in 2011), International Review of Cell and Molecular Biology, Methods in Cell biology, and Molecular and Cellular Oncology (which he co-founded in 2013). Additionally, he serves as Founding Editor for Microbial Cell and Cell Stress, and Associate Editor for Cell Death and Disease, Pharmacological Research and iScience. Nils-Petter Rudqvist received his M.Sc. (Physics, 2009) and Ph.D. (Medical Science, 2015) from the University of Gothenburg, Sweden. He decided to pursue an academic career and continue with his postdoctoral training in US. He first joined the Center for Radiological Research at Columbia University in New York where he studied gene signatures of radiation exposure. He then moved to Weill Cornell Medicine to join the program in radiation and immunity under the mentorship of Dr. Demaria. His current research is focused on investigating which neoantigens are key targets of the radiation-induced anti-tumor T cell response in mice and in patients treated with radiotherapy and immune checkpoint blockade. He recently demonstrated in a mouse model that radiation therapy diversifies the TCR repertoire of tumor infiltrating lymphocytes, an effect crucial for its synergy with immune checkpoint blockade treatment. Nils-Petter has also defined unique patters of expansion of TCR clonotypes in patients who respond or not to treatment with radiotherapy and ipilimumab. He has published 20 articles in peer-reviewed scientific journals and 60+ scientific conference abstracts.

• 1. Cytokine profiling of tumor-infiltrating T lymphocytes by flow cytometryJeremy B. Foote, Sujith Sarvesh and Leisha A. Emens2. High-throughput identification of human antigen-specific CD8+ and CD4+ T cells using soluble pMHC multimersMorgane Magnin, Philippe Guillaume, George Coukos, Alexandre Harari and Julien Schmidt3. In vitro assays for effector T cell functions and activity of immunomodulatory antibodiesRoberta Zappasodi, Sadna Budhu, Mohsen Abu-Akeel and Taha Merghoub4. A cytofluorimetric assay to evaluate T cell polyfunctionalityBelinda Palermo, Mariangela Panetta, Giulia Campo and Paola Nisticò5. Assessment of memory formation by metabolically engineered antigen-specific CD8 T cellsMathias Wenes, Pedro Romero and Lianjun Zhang6. Functional characterization of tumor antigen-specific T-cells isolated from the tumor microenvironment of sleeping beauty induced murine glioma modelsMahmoud S. Alghamri, Felipe J. Núñez, Neha Kamran, Stephen Carney, David Altshuler, Pedro R. Lowenstein and Maria G. Castro7. Methods to edit T cells for cancer immunotherapyFrancesca Lucibello, Silvia Menegatti and Laurie Menger8. Generating stem-like memory T cells with antioxidants for adoptive cell transfer immunotherapy of cancerKarolina Pilipow, Eloise Scamardella and Enrico Lugli9. Reverse immunology: From peptide sequence to tumor-killing human T-cell clonesChristophe Vanhaver, Monica Gordon-Alonso, Alexandre Bayard, Maria Teresa Catanese, Didier Colau, Pierre van der Bruggen and Annika M. Bruger10. Generation of TCR-engineered reference cell samples to control T-cell assay performanceNicole Bidmon, Cécile Gouttefangeas and Sjoerd H. van der Burg11. In vitro expansion of Vγ9Vδ2 T cells for immunotherapyChristian Peters, Léonce Kouakanou, Hans-Heinrich Oberg, Daniela Wesch and Dieter Kabelitz12. CFSE dilution to study human T and NK cell proliferation in vitroIñigo Terrén, Ane Orrantia, Joana Vitallé, Olatz Zenarruzabeitia and Francisco Borrego13. Rapid isolation and enrichment of mouse NK cells for experimental purposesMaite Alvarez, Maria C. Ochoa, Luna Minute, Ignacio Melero and Pedro Berraondo14. Assessment of NK cell-mediated cytotoxicity by flow cytometry after rapid, high-yield isolation from peripheral bloodPeter Holicek, Iva Truxova, Lenka Kasikova, Sarka Vosahlikova, Cyril Salek, Jana Rakova, Monika Holubova, Daniel Lysak, Isabelle Cremer, Radek Spisek and Jitka Fucikova15. Two-dimensional dynamic evaluation of natural killer cell-mediated lysis of adherent target cellsAlexandra Frazao, Louise Rethacker and Anne Caignard16. Rapid isolation of mouse ILCs from murine intestinal tissuesKyle Burrows, Pailin Chiaranunt, Louis Ngai and Arthur Mortha17. Detecting and analyzing murine innate lymphoid cellsAlejandra Gomez-Cadena, Pedro Romero, Sara Trabanelli and Camilla Jandus18. A cytofluorimetric assay to evaluate intracellular cytokine production by NK cellsChristian Sordo-Bahamonde, Seila Lorenzo-Herrero, Segundo González and Alejandro López-Soto19. Applications of microfluidic devices in advancing NK-cell migration studiesXiaoou Ren, Abdulaziz Alamri, Jolly Hipolito, Francis Lin and Sam K.P. Kung20. Complementary approaches to study NKT cells in cancerJay A. Berzofsky, Purevdorj B. Olkhanud and Masaki Terabe21. Assessment of IFN-γ and granzyme-B production by in "sitro" technologyClaudia Galassi, Gwenola Manic, Martina Musella, Antonella Sistigu and Ilio Vitale22. Assessment of IFNγ responsiveness in patient-derived xenograftsJordan J. Cardenas, Camila Robles-Oteiza and Katerina Politi23. Real-time cell analysis (RTCA) to measure killer cell activity against adherent tumor cells in vitroHans-Heinrich Oberg, Christian Peters, Dieter Kabelitz and Daniela Wesch24. Analysis of cancer cell-intrinsic immune regulation in response to CD8+ T cell attackNatalie J. Neubert, Laure Tillé, Christophe Martignier, Silvia A. Fuertes Marraco and Daniel E. Speiser25. A flow cytometry-based method to screen for modulators of tumor-specific T cell cytotoxicityJavier Santos, Jesús Ogando, Rosa Ana Lacalle and Santos Mañes26. Evaluation of NK cell cytotoxic activity against malignant cells by the calcein assaySeila Lorenzo-Herrero, Christian Sordo-Bahamonde, Segundo González and Alejandro López-Soto27. 51Cr-release to monitor NK cell cytotoxicityLeslie Elsner and Ralf Dressel