Neutron Crystallography in Structural Biology

Peter Moody is Professor of Structural Biology at Leicester Institute for Structural & Chemical Biology, Department of Molecular & Cell Biology, University of Leicester, UK

"As one of the first books devoted to this topic, this is a unique resource for structural biologists and students and other trainees interested in this structural probe. It fully meets the high standards of informativeness, breadth and depth of coverage, and clarity of presentation of the Methods in Enzymology series to which it belongs." --Doody

• 1. Fundamentals of neutron crystallography in structural biologyJohn R. Helliwell2. Large crystal growth for neutron protein crystallographyMonika Budayova-Spano, Katarina Koruza and Zoë Fisher3. Prospects for membrane protein crystals in NMXSamuel John Hjorth-Jensen, Esko Oksanen, Poul Nissen and Thomas Lykke-Møller Sørensen4. IMAGINE: The neutron protein crystallography beamline at the high flux isotope reactorFlora Meilleur, Andrey Kovalevsky and Dean A.A. Myles5. The macromolecular neutron diffractometer at the spallation neutron sourceLeighton Coates and Brendan Sullivan6. Current status and near future plan of neutron protein crystallography at J-PARCIchiro Tanaka, Toshiyuki Chatake, Satoru Fujiwara, Takaaki Hosoya, Katsuhiro Kusaka, Nobuo Niimura, Taro Yamada and Naomine Yano7. Neutron macromolecular crystallography at the European spallation sourceMárton Markó, Gergely Nagy, Giuseppe Aprigliano and Esko Oksanen8. Dynamic nuclear polarization enhanced neutron crystallography: Amplifying hydrogen in biological crystalsJoshua Pierce, Matthew J. Cuneo, Ann Jennings, Le Li, Flora Meilleur, Jinkui Zang and Dean A.A. Myles9. Implementation of the riding hydrogen model in CCTBX to support the next generation of X-ray and neutron joint refinement in PhenixDorothee Liebschner, Pavel V. Afonine, Alexandre G. Urzhumtsev and Paul D. Adams10. Interactive model building in neutron macromolecular crystallographyDerek T. Logan11. What are the current limits on determination of protonation state using neutron macromolecular crystallography?Dorothee Liebschner, Pavel V. Afonine, Nigel W. Moriarty and Paul D. Adams12. Proton transfer and drug binding details revealed in neutron diffraction studies of wild-type and drug resistant HIV-1 proteaseAndrey Kovalevsky, Oksana Gerlits, Kaira Beltran, Kevin Weiss, David A. Keen, Matthew P. Blakeley, John M. Louis and Irene T. Weber13. Neutron crystallographic studies of carbonic anhydraseJacob E. Combs, Jacob T. Andring and Robert McKenna14. Protein kinase A in the neutron beam: Insights for catalysis from directly observing protonsOksana Gerlits, Kevin L. Weiss, Matthew P. Blakeley, Gianluigi Veglia, Susan S. Taylor and Andrey Kovalevsky15. Pyridoxal 5’ phosphate dependent reactions: Analyzing the mechanism of　aspartate amino transferaseTimothy C. Mueser, Victoria Drago, Andrey Kovalevsky and Steven Dajnowicz16. The role of hydrogen atoms in redox catalysis by the flavoenzyme cholesterol oxidaseTatiana Kerber and Alice Vrielink17. Heme peroxidase - Trapping intermediates by cryo neutron crystallographyHanna Kwon, Tobias E. Schrader, Andreas Ostermann, Matthew P. Blakeley, Emma L, Raven and Peter C. E. Moody