Tumor Immunology and Immunotherapy - Integrated Methods Part B

Lorenzo Galluzzi is Assistant Professor of Cell Biology in Radiation Oncology at the Department of Radiation Oncology of the Weill Cornell Medical College, Honorary Assistant Professor Adjunct with the Department of Dermatology of the Yale School of Medicine, Honorary Associate Professor with the Faculty of Medicine of the University of Paris, and Faculty Member with the Graduate School of Biomedical Sciences and Biotechnology of the University of Ferrara, the Graduate School of Pharmacological Sciences of the University of Padova, and the Graduate School of Network Oncology and Precision Medicine of the University of Rome “La Sapienza”. Moreover, he is Associate Director of the European Academy for Tumor Immunology and Founding Member of the European Research Institute for Integrated Cellular Pathology.Galluzzi is best known for major experimental and conceptual contributions to the fields of cell death, autophagy, tumor metabolism and tumor immunology. He has published over 450 articles in international peer-reviewed journals and is the Editor-in-Chief of four journals:OncoImmunology (which he co-founded in 2011), International Review of Cell and Molecular Biology, Methods in Cell biology, and Molecular and Cellular Oncology (which he co-founded in 2013). Additionally, he serves as Founding Editor for Microbial Cell and Cell Stress, and Associate Editor for Cell Death and Disease, Pharmacological Research and iScience. Nils-Petter Rudqvist received his M.Sc. (Physics, 2009) and Ph.D. (Medical Science, 2015) from the University of Gothenburg, Sweden. He decided to pursue an academic career and continue with his postdoctoral training in US. He first joined the Center for Radiological Research at Columbia University in New York where he studied gene signatures of radiation exposure. He then moved to Weill Cornell Medicine to join the program in radiation and immunity under the mentorship of Dr. Demaria. His current research is focused on investigating which neoantigens are key targets of the radiation-induced anti-tumor T cell response in mice and in patients treated with radiotherapy and immune checkpoint blockade. He recently demonstrated in a mouse model that radiation therapy diversifies the TCR repertoire of tumor infiltrating lymphocytes, an effect crucial for its synergy with immune checkpoint blockade treatment. Nils-Petter has also defined unique patters of expansion of TCR clonotypes in patients who respond or not to treatment with radiotherapy and ipilimumab. He has published 20 articles in peer-reviewed scientific journals and 60+ scientific conference abstracts.

• 1. A comprehensive approach to gene expression profiling in immune cellsSabrina Carpentier, François Romagné and Eric Vivier2. DIY: Visualizing the immune landscape of tumors using transcriptome and methylome dataSteven F. Gameiro, Farhad Ghasemi, John W. Barrett, James Koropatnick, Anthony C. Nichols, Joe S. Mymryk and Saman Maleki Vareki3. Using epigenetic data to estimate immune composition in admixed samplesAnkur Chakravarthy and Daniel De Carvalho4. Metabolomic approaches to study the tumor microenvironmentGiuseppe Astarita, Suraj Dhungana, Bindesh Shrestha and Evagelia C. Laiakis5. Immunoscore assay for the immune classification of solid tumors: Technical aspects, improvements and clinical perspectivesFlorence Marliot, Lucie Lafontaine and Jérôme Galon6. Transforming growth factor beta (TGF-β) activity in immune-oncology studiesGabriel Gallo-Oller, Marianna Di Scala, Fernando Aranda and Javier Dotor7. Isolation of DNA from exosomesSheila Spada, Nils Rudqvist and Erik Wennerberg8. Decoding cancer cell death-driven immune cell recruitment: An in vivo method for site-of-vaccination analysesShentong Fang, Patrizia Agostinis, Petri Salven and Abhishek D. Garg9. Computational methods in tumor immunologyBhavneet Bhinder and Olivier Elemento10. Deconvoluting tumor-infiltrating immune cells from RNA-seq data using quanTIseqChristina Plattner, Francesca Finotello and Dietmar Rieder11. Concurrent in situ analysis of point mutations and immune infiltrate in FFPE cancersAnn-Marie Baker and Trevor A. Graham12. RNA interference screening methods to identify proliferation determinants and mechanisms of resistance to immune attackYong-Wei Zhang, Rochelle E. Nasto, Sandra A. Jablonski, Ilya G. Serebriiskii, Rishi Surana, Joseph Murray, Michael Johnson, Rebecca B. Riggins, Robert Clarke, Erica A. Golemis and Louis M. Weiner13. An altered miTRAP method for miRNA affinity purification with its pros and consSimon Jasinski-Bergner, Christoforos Vaxevanis, Nadine Heimer, Maria-Filothei Lazaridou, Michael Friedrich and Barbara Seliger14. Identification of immunomodulatory RNA-binding proteins in tumorsMichael Friedrich, Maria-Filothei Lazaridou, Jette Rahn, Christoforos Vaxevanis, Nadine Heimer, Simon Jasinski-Bergner and Barbara Seliger15. Techniques for the generation of humanized mouse models for immuno-oncologyChun I. Yu, Florentina Marches, Te-Chia Wu, Jan Martinek and Karolina Palucka