Craig E. Cameron is at UNC School of Medicine, NC, USA Jamie J. Arnold is at UNC School of Medicine, Chapel Hill, NC, USA

• PrefaceCraig E. Cameron, Jamie J. Arnold and Laurie S. Kaguni1. CoV-er all the bases: Structural perspectives of SARS-CoV-2 RNA synthesisBrandon Malone, Seth A. Darst and Elizabeth A. Campbell2. Mechanisms of inhibition of viral RNA replication by nucleotide analogsKenneth A. Johnson and Tyler Dangerfield3. HCV RdRp, sofosbuvir and beyondJoy Y. Feng and Adrian S. Ray4. Understanding viral replication and transcription using single-molecule techniquesEmmanuelle Pitre and Aartjan J.W. te Velthuis5. In vitro single-molecule manipulation studies of viral DNA replicationRebeca Bocanegra, Ismael Plaza-G.A. and Borja Ibarra6. Allosteric and dynamic control of RNA-dependent RNA polymerase function and fidelityDennis S. Winston and David D. Boehr7. Single-cell analysis for the study of viral inhibitorsMohamad S. Sotoudegan, Jamie J. Arnold and Craig E. Cameron8. Structural basis of viral RNA-dependent RNA polymerase nucleotide addition cycle in picornavirusesPeng Gong9. Picornaviral 2C proteins: A unique ATPase family critical in virus replicationPu Chen, Zhijian Li and Sheng Cui10. Flavivirus enzymes and their inhibitorsEkaterina Knyazhanskaya, Marc C. Morais and Kyung H. Choi11. Watching the bacterial RNA polymerase transcription reaction by time-dependent soak-trigger-freeze X-Ray crystallographyYeonoh Shin and Katsuhiko S. Murakami12. Inhibition of viral RNA-dependent RNA polymerases with clinically relevant nucleotide analogsKieran Maheden, Brendan Todd, Calvin J. Gordon, Egor P. Tchesnokov and Matthias Götte