Ion Channels: Channel Chemical Biology, Engineering, and Physiological Function

Inbunden, Engelska, 2021

2 475 kr

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Beskrivning

Ion Channels, Part C, Volume 653 in the Methods in Enzymology series, highlights new advances in the field with this new volume presenting interesting chapters on a variety of topics, including Nonsense suppression in ion channels, Engineering Ion Channels Using Protein Trans-splicing, Probing Ion Channel Neighborhoods Using APEX, STX based probes for NaVs, ANAP: a versatile, fluorescent probe of ion channel gating and regulation, High Throughput Screens for Small Molecule Ion Channel Modulators, Using toxins to study ion channels, Re/de-constructing ubiquitin regulation of ion channels, Tethered Peptide Toxins for Ion Channels, Voltage-Sensing Phosphatase Molecular Engineering, and more.

Additional chapters cover Engineering excitable cells, Stretch and Poke Stimulation of Mechanically-Activated Ion Channels, Optical Control of STIM Channels, High Throughput Electrophysiological Evaluation of Mutant Ion Channels, Evaluating BEST1 Mutations in RPE Stem Cells, Long Read Transcript Profiling of Ion Channel Splice Variants, Permeation of Connexin Channels, Ratiometric pH indicator for melanosomes and lysosomes, and Ion channels in the epithelial cells of the choroid plexus.

Provides the authority and expertise of leading contributors from an international board of authors
Presents the latest release in the Methods in Enzymology series

Produktinformation

Utgivningsdatum:2021-06-15
Mått:152 x 229 x 25 mm
Vikt:860 g
Format:Inbunden
Språk:Engelska
Serie:Methods in Enzymology
Antal sidor:446
Förlag:Elsevier Science
ISBN:9780128239247

Utforska kategorier

Biokemi inom Naturvetenskap och teknik
Biologi inom Naturvetenskap och teknik

Mer om författaren

Daniel L. Minor, Professor, Cardiovascular Research Institute, University of California San Francisco, San Francisco, CA, USA Henry M. Colecraft, John C. Dalton Professor of Physiology and Cellular Biophysics & Cellular Biophysics, Columbia University College of Physicians & Surgeons, New York, NY, USA

Innehållsförteckning

PrefaceDaniel L. Minor Jr. and Henry M. ColecraftMethods to engineer ion channels to probe different aspects of channel structure-function and regulation.1: Selection and validation of orthogonal tRNA / synthetase pairs for the encoding of unnatural amino acids across kingdoms. Christopher Ahern2: Ion channel engineering using protein trans-splicing. Stephan Alexander Pless3: ANAP: a versatile, fluorescent probe of ion channel gating and regulation. Michael C. Puljung4: Engineering voltage sensing phosphatase, VSP. Yasushi Okamura5: Probing Ion Channel Neighborhoods Using APEX. Steven Marx Methods to control and characterize ion channel function6: Controlling ion channel trafficking by targeted ubiquination and deubiquitination. Henry M. Colecraft 7: Production of recombinant venom peptides as tools for ion channel research. Eitan Reuveny8: Tethered Peptide Toxins for Ion Channels. Steven A. N. Goldstein9: Stretch and poke stimulation for characterizing mechanically activated ion channels. Jörg Grandl10: Optogenetic control of calcium influx in mammalian cells. YUBIN ZHOU11: A method for assessing ionic and molecular permeation in connexin hemichannels. JORGE E. CONTRERAS Methods to characterize ion channel function and expression in physiology and disease12: Choroid plexus epithelial cells as a model to study nongenomic steroid signaling and its effect on ion channel function. Polina V. Lishko13: Live-cell measurements of individual melanosome pH using the novel ratiometric indicator RpHiMEL. Elena Oancea 14: Long Read Transcript Profiling of Ion Channel Splice Isoforms. Liz Tunbridge15: Evaluating BEST1 Mutations in Pluripotent Stem Cell-derived Retinal Pigment Epithelial Cells. Tingting Yang16: High Throughput Electrophysiological Evaluation of Mutant Ion Channels. Alfred L. George Jr. 17: In vitro discovery of novel prokaryotic ion channel candidates for antiarrhythmic gene therapyNenad Bursac