In situ hybridization has become an important and widely used research tool for neurobiologists. This new volume examines the methodological advances that have been made in in situ hybridization techniques and their impact on the study of the nervous system. These include methods for improving sensitivity, ways of examining multiple mRNAs and both proteins and mRNAs within the same section, and methods for using in situ hybridization technology to examine areas of cellular biological significance, such as translational control. The development of alternative labels to radioactivity for the detection of hybridized probes, and the combined use of in situ hybridization and immunocytochemistry is also covered. This volume will be essential to researchers in the neurosciences as well as those in other disciplines.