"I am grateful to Steve Ruzin for assembling this incredibly useful collection of information in such a convenient and handy form." Plant Systematics & Evolution "an archive of expertise in the histochemistry field" Cell Biology International

• 1. Quick Start ; Paraffin Embedding ; Paraffin Embedding - Microwave method ; Glycol methacrylate embedding ; Butyl/methyl methacrylate embedding ; Steedman's wax embedding ; Chromosome squashes ; Hematoxylin staining - microwave technique ; Kohler illumination ; 2. Microscopy ; History of the compound microscope ; Principles of specimen illumination ; 3. Chemical Fixation of Tissues ; The quality of fixation ; The mechanics of fixation ; Coagulating Fixatives ; Crosslinking Agents ; Other fixation methods ; Recommendations for fixing plant tissues ; 4. Tissue Dehydration ; General protocol ; Dehydration using a graded dehydration series ; Dehydration using chemical agents ; 5. Infiltrating Tissues ; Paraffin infiltrating ; Paraffin and PEG Embedding ; Polyethylene glycol and derivatives ; Plastic infiltrating ; 6. Sectioning and Mounting ; Mounting blocks to microtome stubs ; The quality of sectioning ; The microtome knife ; Setting up the microtome ; Sectioning Methods ; Mounting sections to glass slides ; 7. Staining ; Dye Chemistry ; Staining equipment ; General histological staining ; Other microtechniques stains and protocols ; Mounting the coverslip ; Coverslip mounting media ; 8. Alternate Methods of Microtomy ; Free-hand Sections ; Vibratome ; Cryostat ; Sliding microtome ; 9. Special Methods ; Clearing tissues with NaOH and chloral hydrate ; Clearing tissues without removing cytoplasmic components ; Macerating woody tissues ; Macerating non-woody tissues ; Bleaching tissues ; Preserving color in whole mounted specimens ; 10. Problem Solving ; Tissue collection ; Fixation ; Tannins ; Hard materials ; Sliding microtome ; Clearing ; Dehydration, Infiltration, and Embedding ; Microtomy ; Hand sections ; Staining ; 11. Histochemistry and Cytochemistry ; The relationship of the probe to the target ; Cytochemical localization of cell components ; Cytochemical localization of enzymes ; Cytochemical Fluorescence Microscopy ; 12. Localization of Molecular Targets ; Immunolocalization ; Tissue Printing ; in situ hybridization ; APPENDIX I: TOXICS ; APPENDIX II: BUFFERS ; APPENDIX III: COMMON CALCULATIONS ; APPENDIX IV: MERCURY ARC LAMP ; APPENDIX V: MANUFACTURERS AND VENDORS ; APPENDIX VI: OPTICS ; Properties of Light ; General lens optics ; The microscope objective ; The microscope eyepiece ; The condensor ; Fluorescence microscopy ; Microscopy light sources ; BIBLIOGRAPHY ; INDEX

"I am grateful to Steve Ruzin for assembling this incredibly useful collection of information in such a convenient and handy form." Plant Systematics & Evolution "an archive of expertise in the histochemistry field" Cell Biology International

• 1. Quick Start ; Paraffin Embedding ; Paraffin Embedding - Microwave method ; Glycol methacrylate embedding ; Butyl/methyl methacrylate embedding ; Steedman's wax embedding ; Chromosome squashes ; Hematoxylin staining - microwave technique ; Kohler illumination ; 2. Microscopy ; History of the compound microscope ; Principles of specimen illumination ; 3. Chemical Fixation of Tissues ; The quality of fixation ; The mechanics of fixation ; Coagulating Fixatives ; Crosslinking Agents ; Other fixation methods ; Recommendations for fixing plant tissues ; 4. Tissue Dehydration ; General protocol ; Dehydration using a graded dehydration series ; Dehydration using chemical agents ; 5. Infiltrating Tissues ; Paraffin infiltrating ; Paraffin and PEG Embedding ; Polyethylene glycol and derivatives ; Plastic infiltrating ; 6. Sectioning and Mounting ; Mounting blocks to microtome stubs ; The quality of sectioning ; The microtome knife ; Setting up the microtome ; Sectioning Methods ; Mounting sections to glass slides ; 7. Staining ; Dye Chemistry ; Staining equipment ; General histological staining ; Other microtechniques stains and protocols ; Mounting the coverslip ; Coverslip mounting media ; 8. Alternate Methods of Microtomy ; Free-hand Sections ; Vibratome ; Cryostat ; Sliding microtome ; 9. Special Methods ; Clearing tissues with NaOH and chloral hydrate ; Clearing tissues without removing cytoplasmic components ; Macerating woody tissues ; Macerating non-woody tissues ; Bleaching tissues ; Preserving color in whole mounted specimens ; 10. Problem Solving ; Tissue collection ; Fixation ; Tannins ; Hard materials ; Sliding microtome ; Clearing ; Dehydration, Infiltration, and Embedding ; Microtomy ; Hand sections ; Staining ; 11. Histochemistry and Cytochemistry ; The relationship of the probe to the target ; Cytochemical localization of cell components ; Cytochemical localization of enzymes ; Cytochemical Fluorescence Microscopy ; 12. Localization of Molecular Targets ; Immunolocalization ; Tissue Printing ; in situ hybridization ; APPENDIX I: TOXICS ; APPENDIX II: BUFFERS ; APPENDIX III: COMMON CALCULATIONS ; APPENDIX IV: MERCURY ARC LAMP ; APPENDIX V: MANUFACTURERS AND VENDORS ; APPENDIX VI: OPTICS ; Properties of Light ; General lens optics ; The microscope objective ; The microscope eyepiece ; The condensor ; Fluorescence microscopy ; Microscopy light sources ; BIBLIOGRAPHY ; INDEX