Cryopreparation of Thin Biological Specimens for Electron Microscopy

This practical handbook describes the basic principles of electron microscopical cryotechniques. Prior knowledge of electron microscopy is assumed, but not of cryotechniques. The basic principles of electron microscopical cryotechniques are clearly explained in this useful guide. The handbook is written primarily for researchers, postgraduate students and technicians who are familiar with the basics of biological electron microscopy, but have little experience of cryotechniques. It provides firm practical gujidelines for those techniques where the cryofixed specimen is sectioned or otherwise presented as a thin film for examination in the TEM, devoting particular attention to methods of contemporary interest.