Receptor-Ligand Interactions: A Practical Approach

Häftad, Engelska, 1992

Del 92 i serien Practical Approach Series

1 587 kr

Beställningsvara. Skickas inom 7-10 vardagar. Fri frakt över 249 kr.

Beskrivning

This book is the final part of a trilogy of volumes covering the practical aspects of ligand interactions, biochemistry, and effector mechanisms of signal-transducing receptors. The book focuses on the measurement of receptor binding interactions both at equilibrium and from the kinetic point of view. It includes descriptions of the most important techniques for the performance of ligand binding assays to both membrane-bound and solubilized receptors. In addition to which, a systematic effort has been made to provide insight into the strategy and tactics of receptor binding assays, and the conditions which must be satisfied if artifacts are to be avoided and interpretable data obtained. Since the behaviour of even the simplest receptor systems can be surprisingly complex, an attempt has been made to provide some computational tools which allow complications to be predicted and handled or avoided. Ligand binding assays are very widely used, not least in drug screening and development. This volume will help the practitioner to choose an appropriate ligand, make a suitable receptor preparation, design and perform a well thought out set of assays, and analyse the data obtained correctly.

Produktinformation

Utgivningsdatum:1992-05-07
Mått:158 x 235 x 26 mm
Vikt:734 g
Format:Häftad
Språk:Engelska
Serie:Practical Approach Series
Antal sidor:480
Förlag:IRL Press
ISBN:9780199630912

Utforska kategorier

Cellbiologi inom Naturvetenskap och teknik
Farmakologi inom Medicin
Biovetenskap inom Naturvetenskap och teknik

Recensioner i media

'This is an excellent multi-author book. The book represents very good value for the amount of information that it contains and will appeal to both the experienced and those starting receptor research. Selected chapters would be of relevance to undergraduates embarking on final year projects.'Anthony P. Davenport and Peter Molenaar, Addenbrookes Hospital, Trends in Biochemical Sciences, Volume 17 No. 12, December 1992

Innehållsförteckning

Kevin G. McFarthing: Selection and synthesis of receptor-specific radioligands; A.D. Strosberg: Anti-receptor antibodies as ligands; J.O. Dolly: Neurotoxins as receptor ligands; E.C. Hulme & N.J.M. Birdsall: Strategy and tactics in receptor binding studies; E.C. Hulme & N.J. Buckley: Receptor preparations for binding studies; J-X. Wang, H.I. Yamamura, Wan Wang, & W.R. Roeske: The use of the filtration technique in In Vitro radioligand binding assays for membrane-bound and solubilised receptors; E.C. Hulme: Centrifugation binding assays; P.G. Strange: Charcoal adsorption for separating bound and free ligand in radioligand binding assays; E.C. Hulme: Gel filtration assays for solubilised receptors; H. Prinz: Receptor binding kinetics; J.W. Wells: Analysis and interpretation of binding data; Appendix 1: K.G. McFarthing: Radioligands for receptor binding: Amersham International; R.L.Young: Radioli gands for receptor binding: Du Pont-New England Nuclear Corp.