Daniel L. Minor, Professor, Cardiovascular Research Institute, University of California San Francisco, San Francisco, CA, USA Henry M. Colecraft, John C. Dalton Professor of Physiology and Cellular Biophysics & Cellular Biophysics, Columbia University College of Physicians & Surgeons, New York, NY, USA

• PrefaceDaniel L. Minor Jr. and Henry M. ColecraftMethods to produce and purify ion channels1. Effective production of oligomeric membrane proteins by EarlyBac Hiro Furukawa2. Preparation of the Transient Receptor Potential Vanilloid 2 (TRPV2) channel for structural studiesLejla Zubcevic3. Production and Purification of TRPV2 and TRPV5 for Cryo-Electron Microscopy StudiesVera Moiseekova-Bell4. Sample Preparation of the Human TRPA1 Ion Channel for Cryo-EM StudiesSeok-Yong Lee5. Expression and Purification of the Cardiac Sodium Channel NaV1.5 for Cryo-EM Structure DeterminationWilliam A. Catterall6. Structural determination of human Nav1.4 and Nav1.7 using single particle cryo-electron microscopyNieng Yan7. Production and Purification of ATP-Sensitive Potassium Channel Particles for Cryo-Electron MicroscopyShow-Ling Shyng8. Production of K2P2.1 (TREK-1) for structural studiesDaniel L. Minor Jr.9. Purification of a native nicotinic receptorRyan Hibbs10. SARS-CoV-2 3a expression, purification, and reconstitution into lipid nanodiscs Stephen BrohawnOptical methods for probing and controlling channel function11. Studying Ion Channel Conformation Dynamics by Encoding Coumarin as Unnatural Amino AcidSebastian E. Brauchi12. Fluorescence lifetime measurement of absolute membrane potentialEvan Miller13. Fluorescent Toxins as Ion Channel Activity Sensors Jon Sack14. Probing Ion Channel Macromolecular Interactions using Fluorescence Resonance Energy TransferManu Ben-Johny15. Manipulation of Ion Channel Gating with Photoswitchable TweezersThomas Grutter16. Counting Subunits in Kv Channel ComplexesRikard Blunck

Daniel L. Minor, Professor, Cardiovascular Research Institute, University of California San Francisco, San Francisco, CA, USA Henry M. Colecraft, John C. Dalton Professor of Physiology and Cellular Biophysics & Cellular Biophysics, Columbia University College of Physicians & Surgeons, New York, NY, USA

• PrefaceDaniel L. Minor Jr. and Henry M. ColecraftMethods to produce and purify ion channels1. Effective production of oligomeric membrane proteins by EarlyBac Hiro Furukawa2. Preparation of the Transient Receptor Potential Vanilloid 2 (TRPV2) channel for structural studiesLejla Zubcevic3. Production and Purification of TRPV2 and TRPV5 for Cryo-Electron Microscopy StudiesVera Moiseekova-Bell4. Sample Preparation of the Human TRPA1 Ion Channel for Cryo-EM StudiesSeok-Yong Lee5. Expression and Purification of the Cardiac Sodium Channel NaV1.5 for Cryo-EM Structure DeterminationWilliam A. Catterall6. Structural determination of human Nav1.4 and Nav1.7 using single particle cryo-electron microscopyNieng Yan7. Production and Purification of ATP-Sensitive Potassium Channel Particles for Cryo-Electron MicroscopyShow-Ling Shyng8. Production of K2P2.1 (TREK-1) for structural studiesDaniel L. Minor Jr.9. Purification of a native nicotinic receptorRyan Hibbs10. SARS-CoV-2 3a expression, purification, and reconstitution into lipid nanodiscs Stephen BrohawnOptical methods for probing and controlling channel function11. Studying Ion Channel Conformation Dynamics by Encoding Coumarin as Unnatural Amino AcidSebastian E. Brauchi12. Fluorescence lifetime measurement of absolute membrane potentialEvan Miller13. Fluorescent Toxins as Ion Channel Activity Sensors Jon Sack14. Probing Ion Channel Macromolecular Interactions using Fluorescence Resonance Energy TransferManu Ben-Johny15. Manipulation of Ion Channel Gating with Photoswitchable TweezersThomas Grutter16. Counting Subunits in Kv Channel ComplexesRikard Blunck