Selenoprotein Structure and Function

Eranthie Weerapana is an Associate Professor of Chemistry at Boston College. She received her B.S. in Chemistry from Yale University, and her Ph.D. in Chemistry from MIT, where she worked with Professor Barbara Imperiali, investigating glycosyltransferases involved in N-linked glycosylation in the gram negative bacterium Campylobacter jejuni. She then performed postdoctoral studies at The Scripps Research Institute, La Jolla where she worked with Professor Benjamin F. Cravatt to develop chemical-proteomic methods to investigate reactive cysteines in complex proteomes. Her interdisciplinary research program focuses on applying mass-spectrometry methods to identify regulatory cysteine residues in the human proteome, and chemical biology approaches to develop covalent small-molecule modulators for cysteine-mediated protein activities.

• 1. Identification of Selenoprotein O substrates using a biotinylated ATP analogAnju Sreelatha2. Selenium-encoded isotopic signature targeted profilingChu Wang3. Designing tRNASec variants for efficient selenocysteine incorporation using Sec-specific reportersDieter Söll4. Preparation of selenoprotein S by chemical ligationSharon Rozovsky5. Examining xCT-mediated selenium uptake and selenoprotein production capacity in cellsDohoon Kim6. SecMS analysis of selenoprotein with selenocysteine insertion sequence and beyondYaoyang Zhang7. Selenocysteine substitutions in thiyl radical enzymesBrandon Greene8. Recombinant selenoprotein expression in E. coli based upon the redefinition of a UAG codon in an RF1-depleted host strainElias S.J. Arnér9. Metabolic labeling with radioactive selenium in　zebrafishPaul Copeland10. Low pH isoTOP-ABPP to identify selenocysteinesDaniel Bak11. Expression of selenoproteins via genetic code expansion in mammalian cellsJennifer Peeler12. Alpha-methyl selenocysteine as a tool for the study of selenoproteinsRobert Hondal13. Selective selenol fluorescent probes: design, synthesis, structural determinants, and biological applicationsJianguo Fang and Baoxin Zhang14. Diselenide-selenoester ligationRichard Payne15. Modeling selenocysteine-derived reactive intermediates utilizing a molecular cradleKei Goto16. Methods for accurate and reproducible studies of pharmacological effects of selenium in cancerMikael Björnstedt