Correlative Light and Electron Microscopy V

Thomas Müller-Reichert works in the Core Facility Cellular Imaging (CFCI), Faculty of Medicine Carl Gustav Carus, Technische Universität Dresden, Germany. Paul Verkade is a Professor of Bioimaging at the University of Bristol, UK where his research group works on the development and application of microscopy techniques to Biomedical questions. The main tools in the lab are Electron microscopy (EM) and Correlative Light Electron Microscopy (CLEM) in which fields he has published over 100 papers and edited 5 books on CLEM (including 4 Volumes of the Methods in Cell Biology series). PV obtained his PhD at the University of Utrecht, The Netherlands in 1996. Subsequently he did a post-doc at the EMBL, Heidelberg, Germany, after which he set up the electron microscopy unit at the newly formed Max Planck Institute for Molecular Cell Biology in Dresden, Germany from 2001. He moved to the UK in 2006 to set up another EM unit as part of an integrated LM and EM bioimaging facility, which facilitates CLEM workflows. He is actively involved in shaping the future microscopy landscape with roles in the Royal Microscopical Society and BioimagingUK and a current focus on putting volumeEM on the imaging map through community building and the organisation and co-chairing of the 1st Gordon Research Conference on vEM.

• 1. How to Apply the Broad Toolbox of Correlative Light and Electron Microscopy to Address a Specific Biological QuestionThomas Müller-Reichert, Erin M. Tranfield, Gunar Fabig and Thomas Kurth2. Some tips and tricks for a correlative light electron microscopy workflow using stable expression of fluorescent proteinsPaul Verkade3. Targeting membrane receptors with fluoronanogold probes for high resolution correlative microscopy.Monica Fernandez Monreal4. Correlative light and electron microscopy at defined cell cycle stages in a controlled environmentShotaro Otsuka5. Severe acute respiratory syndrome coronavirus 2 (SARS-CoV2) culture and sample preparation for correlative light electron microscopyPaul Verkade, Maximillian Erdmann, Isobel Web, Lorna Hodgson and Andrew Davidson6. Tissue CLEMChristine Longin7. Array tomography of in vivo labeled synaptic receptorsChristian Stigloher and Sebastian Britz8. Correlative cryo-microscopy pipelines for in situ cellular studiesAnna Sartori and Chiara Zurzolo9. Building a super-resolution fluorescence cryomicroscopeThom Sharp10. Analysis of super resolution CLEM dataThom Sharp11. Nanometer targeting using cryo super resolutionLaura Carolina Zanetti-Domingues12. Laboratory Based Correlative Cryo-Soft X-Ray Tomography and Cryo-Fluorescence MicroscopyKenneth Fahy