Michael grew up in a western suburb of Chicago called Aurora, IL, or better known as Wayne’s World. He got his BS in Psychology and Chemistry at the University of Illinois, Urbana-Champaign. After which, he employed as a technician working on analytical method development at Abbott Laboratories. From 1998-2002 he was a PhD student at Penn State University working in Stephen Benkovic lab on the Bacteriophage T4 replisome assembly and dynamics. In 2003, he started a postdoctoral fellowship in Ron Laskey’s laboratory at the University of Cambridge/Hutchison MRC in the UK. In 2006, he started his independent career at the University of Pittsburgh Chemistry department. Starting 2014, he moved his laboratory to Baylor University, where he currently lives with his family. There, the Trakselis laboratory is currently studying aspects of DNA replication and repair spanning from the very in vitro biochemical to the very in vivo cell and human biology.

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• 1. Crosslinking immunoprecipitation of Upf1 in yeastKristian Baker2. Biochemical Analysis of the SARS-CoV-2 Nsp13 Helicase Implicated in COVID-19Robert Brosh3. Bulk phase biochemistry of the Pif1 helicaseMatthew Bochman4. Translational control by helicases during cellular stressTim Bolger5. Measuring DDX3-dependent protein synthesis in vitro and in cellsStephen Floor6. Purification of Mtr4 and Mtr4-mediated complexesSean Johnson7. Plan of attack for studying DEAD-box proteinsKatrin Karbstein8. Reconstitution, purification, and activity characterization for RNA-guided helicase-nuclease machines in Type I CRISPR-Cas systemsAilong Ke9. Helicase mediated vectorial folding of G-quadruplexSua Myong10. Detecting G4 quadruplex unwindingKatrin Paeschke11. Monitoring RNA and ATP-dependent conformational changes in RIG-I-like receptors using fluorescence microscopyAnna Marie Pyle12. ATP utilization measurements in RNA unwinding and RNA chaperone activities by helicase proteinsRick Russell13. Hydrogen-Deuterium Exchange Mass Spectrometry of RNA HelicasesSheena D'Arcy14. Biochemical characterization of human MTR4-RNA exosome and adaptor complexesChristopher D. Lima15. Study of RNA helicases with magnetic tweezersHerve Le Hir16. Single-molecule FRET to analyze the dynamics of RNA helicasesDagmar Klostermeier17. Transcriptional regulation by a RecQ helicaseSudha Sharma18. In vitro characterization of the DEXH helicase Dhr1 from yeastArlen Johnson

Michael grew up in a western suburb of Chicago called Aurora, IL, or better known as Wayne’s World. He got his BS in Psychology and Chemistry at the University of Illinois, Urbana-Champaign. After which, he employed as a technician working on analytical method development at Abbott Laboratories. From 1998-2002 he was a PhD student at Penn State University working in Stephen Benkovic lab on the Bacteriophage T4 replisome assembly and dynamics. In 2003, he started a postdoctoral fellowship in Ron Laskey’s laboratory at the University of Cambridge/Hutchison MRC in the UK. In 2006, he started his independent career at the University of Pittsburgh Chemistry department. Starting 2014, he moved his laboratory to Baylor University, where he currently lives with his family. There, the Trakselis laboratory is currently studying aspects of DNA replication and repair spanning from the very in vitro biochemical to the very in vivo cell and human biology.

Select Guide Rating

• 1. Crosslinking immunoprecipitation of Upf1 in yeastKristian Baker2. Biochemical Analysis of the SARS-CoV-2 Nsp13 Helicase Implicated in COVID-19Robert Brosh3. Bulk phase biochemistry of the Pif1 helicaseMatthew Bochman4. Translational control by helicases during cellular stressTim Bolger5. Measuring DDX3-dependent protein synthesis in vitro and in cellsStephen Floor6. Purification of Mtr4 and Mtr4-mediated complexesSean Johnson7. Plan of attack for studying DEAD-box proteinsKatrin Karbstein8. Reconstitution, purification, and activity characterization for RNA-guided helicase-nuclease machines in Type I CRISPR-Cas systemsAilong Ke9. Helicase mediated vectorial folding of G-quadruplexSua Myong10. Detecting G4 quadruplex unwindingKatrin Paeschke11. Monitoring RNA and ATP-dependent conformational changes in RIG-I-like receptors using fluorescence microscopyAnna Marie Pyle12. ATP utilization measurements in RNA unwinding and RNA chaperone activities by helicase proteinsRick Russell13. Hydrogen-Deuterium Exchange Mass Spectrometry of RNA HelicasesSheena D'Arcy14. Biochemical characterization of human MTR4-RNA exosome and adaptor complexesChristopher D. Lima15. Study of RNA helicases with magnetic tweezersHerve Le Hir16. Single-molecule FRET to analyze the dynamics of RNA helicasesDagmar Klostermeier17. Transcriptional regulation by a RecQ helicaseSudha Sharma18. In vitro characterization of the DEXH helicase Dhr1 from yeastArlen Johnson

Michael grew up in a western suburb of Chicago called Aurora, IL, or better known as Wayne’s World. He got his BS in Psychology and Chemistry at the University of Illinois, Urbana-Champaign. After which, he employed as a technician working on analytical method development at Abbott Laboratories. From 1998-2002 he was a PhD student at Penn State University working in Stephen Benkovic lab on the Bacteriophage T4 replisome assembly and dynamics. In 2003, he started a postdoctoral fellowship in Ron Laskey’s laboratory at the University of Cambridge/Hutchison MRC in the UK. In 2006, he started his independent career at the University of Pittsburgh Chemistry department. Starting 2014, he moved his laboratory to Baylor University, where he currently lives with his family. There, the Trakselis laboratory is currently studying aspects of DNA replication and repair spanning from the very in vitro biochemical to the very in vivo cell and human biology.

Select Guide Rating

• 1. Crosslinking immunoprecipitation of Upf1 in yeastKristian Baker2. Biochemical Analysis of the SARS-CoV-2 Nsp13 Helicase Implicated in COVID-19Robert Brosh3. Bulk phase biochemistry of the Pif1 helicaseMatthew Bochman4. Translational control by helicases during cellular stressTim Bolger5. Measuring DDX3-dependent protein synthesis in vitro and in cellsStephen Floor6. Purification of Mtr4 and Mtr4-mediated complexesSean Johnson7. Plan of attack for studying DEAD-box proteinsKatrin Karbstein8. Reconstitution, purification, and activity characterization for RNA-guided helicase-nuclease machines in Type I CRISPR-Cas systemsAilong Ke9. Helicase mediated vectorial folding of G-quadruplexSua Myong10. Detecting G4 quadruplex unwindingKatrin Paeschke11. Monitoring RNA and ATP-dependent conformational changes in RIG-I-like receptors using fluorescence microscopyAnna Marie Pyle12. ATP utilization measurements in RNA unwinding and RNA chaperone activities by helicase proteinsRick Russell13. Hydrogen-Deuterium Exchange Mass Spectrometry of RNA HelicasesSheena D'Arcy14. Biochemical characterization of human MTR4-RNA exosome and adaptor complexesChristopher D. Lima15. Study of RNA helicases with magnetic tweezersHerve Le Hir16. Single-molecule FRET to analyze the dynamics of RNA helicasesDagmar Klostermeier17. Transcriptional regulation by a RecQ helicaseSudha Sharma18. In vitro characterization of the DEXH helicase Dhr1 from yeastArlen Johnson