Sedimentation Velocity Analytical Ultracentrifugation

Dr. Schuck obtained his Ph.D. from the Goethe-University Frankfurt am Main, Germany, where he worked on interactions of integral proteins of the erythrocyte membrane using analytical ultracentrifugation. He received his post-doctoral research training in physical biochemistry with Dr. Allen Minton at NIDDK, and joined the Bioengineering and Physical Science Program of NCRR as a Research Fellow in 1997. He is currently a Earl Stadtman Tenure-Track Investigator and Chief of the Dynamics of Macromoleular Assembly Section, Laboratory of Cellular Imaging and Macromolecular Biophysics at the National Institute of Biomedical Imaging and Bioengineering, National Institutes of Health.

"The beauty of this book is that it addresses, in just one place, theory, experiments, and computing in application to analytical ultracentrifugation (AUC) performed with biological and soft matter systems. As the author states, it is the ability to compute routine solutions to the Lamm equation of sedimentation that has transformed the capabilities of modern AUC experiments, and the Lamm equation is the cornerstone of this book. The reader is taken through a comprehensive mathematical tour of how to fit sedimentation coefficient distribution functions c(s) in over 200 pages. Following a recap of the basics at the start, we are presented with the main scenarios of how sedimentation is analyzed. This starts with discrete non-interacting particles and the distributions resulting from these, then it moves to consider the distributions that result from non-diffusing and diffusing particles. There are useful discourses into g*(s) analyses and multi-component distributions, and a neat touch is the inclusion of practical considerations in the fits at the end. There is a summary at the end of how the Lamm equation works. Irrespective of what type of system is being worked with, this authoritative text gives insight of how the appropriate analysis tools work. This is a truly excellent resource for any ultracentrifugation laboratory to consult when needed, with well-focused sections, and I will certainly be using it myself!"—Steve Perkins, University College London, UK"Covering modern sedimentation velocity methodologies for a number of possible solution systems, this new book is comprehensive, accurate, and precise. Based on a very strong mathematical background and including brief tutorials, one will be able to use this book as a dictionary of computer calculation-based modern sedimentation velocity methods, and refer to it even in 20 or 30 years."—Susumu Uchiyama, Osaka University, Japan

• Basic Analysis PrinciplesSedimentation of Discrete Non-Interacting ParticlesProperties of Sedimentation Coefficient DistributionsDistributions Of Non-Diffusing ParticlesDistributions of Diffusing ParticlesSedimentation Coefficient Distributions from Boundary Derivatives and ExtrapolationsMulti-Component DistributionsPractical Analysis of Non-Interacting SystemsAppendix A: Numerical Solutions of the Lamm EquationAppendix B: Calculating Distributions