Steroid Biochemistry

Dr. Trevor Penning, the Thelma Brown and Henry Charles Molinoff Professor of Pharmacology, is a professor of Systems Pharmacology and Translational Therapeutics at the University of Pennsylvania and has been the Director of the Center of Excellence in Environmental Toxicology (CEET) since its founding in 2006. He formulated the strategic vision of the CEET from its inception and built the environmental health science identity of the CEET at the University, regional, and national level. As Director of the CEET, Dr. Penning has focused the center’s research around the connections between human disease and environmental exposures and emphasized the importance of using this research to mitigate health impacts on vulnerable communities both locally and globally.Throughout his time as Director, Dr. Penning has focused his research on the connections between human health and anthropogenic pollution. Air pollution is responsible for more than 200,000 deaths globally each year and has been identified as a known human carcinogen by the World Health Organization.

• Section I. Cytochrome P450 Enzymes 1. Steroidogenic P450s (CYP11A1, 17A1, 21A2, 11B1, and 11B5)Emily Scott2. Steroid 17alpha-hydroxylase/17,20-lyase (Cytochrome P450 17A1)F. Peter Guengerich, Yasuhiro Tateishi, Kevin D. McCarty and Lu LiuSection 2. Enzymes of Estrogen Biosynthesis 3. Aromatase and Steroid SulfataseDebashi Ghosh4. Estrogenic 17b-Hydroxysteroid DehydrogenaseSection 3. Hydroxysteroid Dehydrogenases (HSD) 5. 3a-Hydroxyssteroid Dehydrogenase(s)6. Approaches to Measuring 3b-Hydroxysteroid Dehydrogenase Type 1Nima Sharifi7. 3b-Hydroxysteroid Dehydrogenase Type 28. 11b-Hydroxysteroids Dehydrogenase Type 1Alex Odermatt9. 11b-Hydroxysteroids Dehydrogenase Type 2Alex Odermatt10. 17b-Hydroxysteroid DehydrogenasesTea Lanisnik RiznerSection 4. Steroid Reductases 11. Approaches for evolutionary, biochemical, and structural analysis of bacterial steroid 5a-reductasesRen Ruobing12. Mammalian Steroid 5a-ReductaseRichard J. Auchus13. Human Steroid 5b-Reductase (AKR1D1)Trevor Martin Penning14. Human Aldo-Keto Reductases (AKR1C1-AKR1C4)-Cloning & Expression of Recombinant EnzymesTrevor Martin Penning and Andrea DetlefsenSection 5. Steroid Conjugating Enzymes 15. Steroid sulfotransferases-X SULT2A1, 2B1b and 1E1Charles Napoleon Falany16. Steroid UGT-Glucuronsyl TransfereasesSection 6. Steroid Analytical Chemistry 17. Mass spectrometry of Glucocorticiids18. Mass spectrometry of Progestins19. LC-MS Quantitation of Hydroxy- and Keto-AndrogensClementina Mesaros, Andrea Detlefsen and Ryan Paulukinas20. Quantification of 11-oxygenated androgens by RP-UV-HPLC post 2,4-dinitrophenylhydrazine derivatizationRyan Paulukinas21. 11-Oxygenated AndrogensAmanda Swart22. LC-MS Quantitation of EstrogensClementina Mesaros