Epigenetics Methods, Second Edition offers an extensive overview of the tools that enhance translational epigenetics studies. This edition includes updated and revised chapters, along with newly added topics such as MALDI-TOF-MS for analyzing DNA methylation and the RIP assay for lncRNA binding. Specialists provide step-by-step guidance on methods used to study various epigenetic mechanisms. The book covers both fundamental and advanced techniques, making it an essential resource for researchers in this dynamic field. In addition to DNA methylation and histone modification techniques, the book also explores chromatin evaluation, enzyme analyses, and non-coding RNAs as epigenetic modulators.New chapters discuss recent advancements like single-cell epigenomics, epigenetic editing, and computational epigenetics. The volume addresses reproducibility issues and offers consensus-driven methods to unify approaches across experiments and labs. This edition is a vital reference for understanding and employing cutting-edge epigenetics methods. - Provides revised and updated chapters, as well as new chapters covering recent developments- Includes contributions by leading international investigators involved in epigenetic research and clinical and therapeutic application- Integrates technology and translation with fundamental chapters on epigenetics methods, as well as chapters on more novel and advanced epigenetics methods- Written at verbal and technical levels that can be understood by scientists and students alike- Includes chapters on state-of-the-art techniques such as single-cell epigenomics, use of CRISPR/Cas9 for epigenetic editing, and epigenetics methods applied to forensics
