Mononuclear Non-heme Iron Dependent Enzymes

After completing studies for the A.B., A.M., and Ph.D. degrees in chemistry at Harvard University, David W. Christianson joined the faculty of the University of Pennsylvania, where he is currently the Roy and Diana Vagelos Professor in Chemistry and Chemical Biology. At Penn, Christianson’s research focuses on the structural and chemical biology of the zinc-dependent histone deacetylases as well as enzymes of terpene biosynthesis. His research accomplishments have been recognized by several awards, including the Pfizer Award in Enzyme Chemistry and the Repligen Award in Chemistry of Biological Processes from the American Chemical Society, a Guggenheim Fellowship, and the Elizabeth S. and Richard M. Cashin Fellowship from the Radcliffe Institute for Advanced Study at Harvard University. Christianson is also a dedicated classroom teacher, and his accomplishments in this regard have been recognized by the Lindback Award for Distinguished Teaching at Penn and a Rhodes Trust Inspirational Educator Award from Oxford University. Christianson has also held visiting professorships in the Department of Biochemistry at Cambridge University and the Department of Chemistry and Chemical Biology at Harvard University. Christianson has served with Prof. Anna Pyle as Co-Editor-in-Chief of Methods in Enzymology since 2015.

• 1. Photoreduction for Rieske oxygenase chemistryPinghua Liu2. Insights into the Mechanisms of Rieske Oxygenases from Studying the Unproductive Activation of DioxygenThomas B. Hofstetter, Charlotte E. Bopp and Nora M. Bernet3. Non-heme iron and 2-oxoglutarate enzymes catalyze cyclopropane and azacyclopropane formationsWei-chen Chang4. Obtaining precise metrics of substrate positioning in Fe(II)/2OG dependent enzymes using Hyperfine Sublevel Correlation SpectroscopyAlexey Silakov5. Xe-pressurization studies for revealing substrate-entrance tunnelsJennifer D. Bridwell-Rabb6. A tale of two dehydrogenases involved in NADH recyclingJennifer D. Bridwell-Rabb7. Rieske oxygenases and/or their partner reductase proteinsPravindra Kumar8. Expression, assay and inhibition of 9-cis-epoxycarotenoid dioxygenase (NCED) from Solanum lycopersicum and Zea maysTimothy David Bugg9. Biocatalysis and non-heme iron enzymesXiongyi Huang10. In vitro analysis of the three-component Rieske oxygenase cumene dioxygenase from Pseudomonas fluorescens IP01Sandy Schmidt and Niels A.W. de Kok11. Structure and function of carbazole 1,9a-dioxygenaseHideaki Nojiri12. Characterization of a Mononuclear Nonheme Iron-dependent Mono-oxygenase OzmD in Oxazinomycin BiosynthesisHung-wen Liu13. Spectroscopic and computational studies of a bifunctional iron- and 2-oxoglutarate dependent enzyme, AsqJYisong Guo14. Non-heme iron oxygenases for natural product biosynthesisIkuro Abe15. Isonitrile Formation by a Non-Heme Iron(II)-Dependent Oxidase/DecarboxylaseWenjun Zhang16. Assays for the epidermal growth factor hydroxylase AsphChristopher Joseph Schofield17. Amino acid hydroxylasesPaul Fitzpatrick18. Extradiol dioxygenasesYifan Wang19. Title to be determinedRamaswamy Subramanian20. Experimental and computational approaches to understanding and addressing the stability limitations of Rieske OxygenasesJennifer DuBois21. Construction and Testing of Whole-Cell Non-Heme Iron BiocatalystsRyan Summers22. Equilibrium dialysis to measure binding affinities of non-heme iron enzymesAmbika Bhagi-Damodaran23. title to be determinedThomas C. Brunold24. Approaches to determination of the mechanism of the Rieske monooxygenase salicylate 5-hydroxylaseJohn D. Lipscomb and Melanie Rogers25. Preparation of reductases for multicomponent oxygenasesLindsay David Eltis and Megan Wolf26. Expression, purification, and biochemical characterization of micro- and macroalgal kainoid synthases Shaun McKinnie27. Title to be determinedKabirul Islam28. Title to be determinedEdward I. Solomon29. title to be detrminedAdam Offenbacher