CRISPR-Cas Systems for RNA and Genome Editing

Inbunden, Engelska, 2025

2 184 kr

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Beskrivning

CRISPR-Cas Systems for RNA and Genome Editing, Volume 711 in this ongoing series, highlights new advances in the field, with this new volume presenting interesting chapters on topics such as Massively parallel genomic perturbations with multi-target CRISPR interrogates Cas9 activity and DNA repair at endogenous sites, Genome oligopaint via local denaturation fluorescence in situ hybridization, Biochemical reconstitution of a type I-B CRISPR-associated transposon, Visualizing the conformational landscape of CRISPR-Cas9 through kinetics-informed structural studies, and much more.

Additional sections cover Analysis of metal-dependent DNA nicking activities by Cas endonucleases, Biochemically characterizing the accessory proteins and ribonucleoprotein complexes of Type IV CRISPR systems, Prime editing in bacteria with BacPE, Preparation of high-purity CRISPR-based DNA base editor RNPs, Bacterial directed evolution of CRISPR base editors, Cloning and validating systems for high throughput molecular recording, Using CRISPR-Cas13 for viral nucleic acid detection, and more.

Provides the latest information on DNA/RNA research
Offers outstanding and original reviews on a range of DNA/RNA research topics
Serves as an indispensable reference for researchers and students alike

Produktinformation

Utgivningsdatum:2025-06-02
Mått:152 x 229 x 32 mm
Vikt:1 070 g
Format:Inbunden
Språk:Engelska
Serie:Methods in Enzymology
Antal sidor:576
Förlag:Elsevier Science
ISBN:9780443317521

Utforska kategorier

Biokemi inom Naturvetenskap och teknik
Biologi inom Naturvetenskap och teknik

Innehållsförteckning

1. Massively parallel genomic perturbations with multi-target CRISPR interrogates Cas9 activity and DNA repair at endogenous sitesTJ Ha2. Genome oligopaint via local denaturation fluorescence in situ hybridizationTJ Ha3. TBDOsamu Nureki4. Biochemical reconstitution of a type I-B CRISPR-associated transposonLeifu Chang5. TBDYibei Xiao6. Visualising the conformational landscape of of CRISPR-Cas9 through kinetics-informed structural studiesDavid W. Taylor7. Analysis of metal-dependent DNA nicking activities by Cas endonucleasesDipali G. Sashital8. Biochemically characterizing the accessory proteins and ribonucleoprotein complexes of Type IV CRISPR systems.Ryan Jackson9. HYERJunjie (Gogo) liu10. focused on the purification and characterization of gRAMP, as well as its application in GFP knockdown in E. coli.Chunyi Hu11. TBDPeter Fineran12. Cas12a2 ?Chase Beisel13. Prime editing in bacteria with BacPEQuanjiang Ji14. TBDJia Chen15. Preparation of high-purity CRISPR-based DNA base editor RNPsAudrone Lapinaite16. Bacterial directed evolution of CRISPR base editorsShannon Miller17. Cloning and validating systems for high throughput molecular recordingMichelle Chan18. TBDSamagya Banskota19. Using CRISPR-Cas13 for viral nucleic acid detectionCameron Myhrvold20. CRISPR-based epigenetic editing for programmable gene silencing in mammalian cellsJames Nuñez21. Genome editing with programmable base editors in human cells” – explaining how to pick out and validate BE:gRNA combinations for a given target, with tips and tricks for maximizing editing efficienciesAlexis Komor22. High-throughput evaluation of genetic variants using base/prime editing sensor librariesFrancisco J. Sánchez Rivera23. TBDJia Chen