Dr. Chandrashekhar Singh is a Research Associate at the Department of Pharmaceutical Engineering and Technology, Indian Institute of Technology, Banaras Hindu University, Varanasi, Uttar Pradesh, India. Dr. Singh earned his Ph.D. in Biotechnology from the Department of Botany, Banaras Hindu University. Dr. Singh has authored 19 research articles that have been published in reputable international journals. Additionally, he has written 4 book chapters in collaboration with renowned science publishers. Dr. Singh's primary research focuses on the development of bio-inspired nanoparticles and their applications as antibacterial in the management of wound infections and plant Biotechnology. His contributions to this field have earned him recognition, including an Indian patent. Furthermore, Dr. Singh is actively engaged in the preparation of more research and review articles, which are currently in the process of publication. Dr. Singh also contributes to the academic community as a reviewer for esteemed journals such as Vegetos (Springer). Dr. Akhileshwar Kumar Srivastava works as a Research Associate (ICMR) at CSIR-Central Food Technological Research Institute, Mysore, India. In his 11 years of research, he has published more than 18 research articles in reputed international journals, along with 4 books and 11 book chapters. His research specialization is primarily in the area of pharmacognosy with genetics, metabolomics, bioinformatics, and molecular biology associated with targeting virulent factors of diseases. In addition, he has studied at Augusta University (formerly, Georgia Regents University) in Augusta, GA, USA, on a J-1 Exchange Scholar Visa and at Ben-Gurion University, Israel. He is also a life member fellow in the Indian Science Congress and the Agriculture, Nutrition and Health Academy, UK. Dr. Anbarasu Kannan is a senior scientist and assistant professor in the Department of Biochemistry, CSIR-CFTRI, Mysuru, Karnataka, India. He had previous training in both Doctorate (2013) and post-doctoral (from 2014-2017) in cancer research. During post-doctoral training, he has been working on exosome biogenesis, mitochondrial genetic alterations, and dynamics in different cancers. His work has been published in peer-reviewed journals. He has been awarded the Early career Research Award (ECRA) in tracing the alteration signature of SH3GL2 in circulating exosomes of breast cancer progression to develop exosome-based biomarker. He has research expertise in the field of exosome biogenesis and mitochondrial dynamics, and in development of exosome biomarkers for cancer diagnostics and therapeutics.Dr. Deepjyoti Singh is a Postdoctoral Researcher at the Department of Entomology and Plant Pathology, North Carolina State University, Raleigh, North Carolina, USA. With over a decade of expertise in molecular biology, her work focuses on plant-virus-vector interactions. She earned her Ph.D. from the School of Life Sciences, Jawaharlal Nehru University, New Delhi, India, where she studied the mechanism of systemic acquired resistance in plants. During her tenure as a postdoctoral fellow at Syracuse University, New York, USA, she worked on understanding the epigenetic mechanism of plant defense in Arabidopsis. She has published 8 research articles in reputable international journals and presented her work at various national and international conferences. In addition to her research, Dr. Singh contributes as a reviewer for esteemed journals, including Molecular Plant-Microbe Interactions (MPMI) and Frontiers in Microbiology.Dr. Sunil L is currently working as a postdoctoral fellow at the Department of Pharmacological and Pharmaceutical Sciences at the University of Houston College of Pharmacy, Houston, TX, USA. He mainly focuses on small molecules of the Munc13.1.C1 domain and the Munc13.1.C1.C2B domain of presynaptic CNS protein, intending to develop drugs for alcohol addiction and Alzheimer’s disease. His research focuses on protein expression, purification, characterization, and molecular simulation of Munc13.1 presynaptic protein and its interaction with small molecules. He has published 22 research articles in Web of Science indexed journals.

• Section 1 Extracellular vesicles/exosomes as novel sources of cellular communication1. Historical context and the evolution of exosome research2. The cellular machinery behind exosome formation3. Exosomes as messengers: intercellular communication4. Exosomes: essential messengers in intercellular communication5. Exosomes as cellular couriers: the mechanisms of intercellular messagingSection 2 Extracellular vesicles/exosomes: methods and protocols6. Comprehensive approaches to isolation, characterization, and functional assessment of extracellular vesiclesSection 3 Extracellular vesicles/exosomes in health and diseases: Potential applications7. Exosomes as biomarkers for disease diagnosis8. The intricate interplay between cancer cells and exosomes9. Advancements in stem cell therapy facilitated by exosomes10. Crossing the blood–brain barrier: exosomes in neurological research11. Exosomes as vectors across the blood–brain barrier in neurological research12. Unraveling the role of exosomes in modulating immune responses13. The immunological impact of exosomes: modulation and mechanisms14. Exploring the link between exosomes and metabolic diseases15. Role of exosomal microRNAs in anticancer drug resistance and tumor angiogenesis16. Health implications of retinoic acid in exosome biogenesis, secretion, and uptake17. Exosomes in aging and longevitySection 4 Engineering and scaling up extracellular vesicles and exosomes production: Medicine, nutraceuticals, cosmetics, and novel applications18. Cutting-edge technology for extracellular vesicles and exosomes production: future prospects19. Novel application of extracellular vesicles and exosomes in nutraceutical and cosmetic production20. Ethical considerations, challenges, and the road ahead: exosomes in the next decade

Dr. Chandrashekhar Singh is a Research Associate at the Department of Pharmaceutical Engineering and Technology, Indian Institute of Technology, Banaras Hindu University, Varanasi, Uttar Pradesh, India. Dr. Singh earned his Ph.D. in Biotechnology from the Department of Botany, Banaras Hindu University. Dr. Singh has authored 19 research articles that have been published in reputable international journals. Additionally, he has written 4 book chapters in collaboration with renowned science publishers. Dr. Singh's primary research focuses on the development of bio-inspired nanoparticles and their applications as antibacterial in the management of wound infections and plant Biotechnology. His contributions to this field have earned him recognition, including an Indian patent. Furthermore, Dr. Singh is actively engaged in the preparation of more research and review articles, which are currently in the process of publication. Dr. Singh also contributes to the academic community as a reviewer for esteemed journals such as Vegetos (Springer). Dr. Akhileshwar Kumar Srivastava works as a Research Associate (ICMR) at CSIR-Central Food Technological Research Institute, Mysore, India. In his 11 years of research, he has published more than 18 research articles in reputed international journals, along with 4 books and 11 book chapters. His research specialization is primarily in the area of pharmacognosy with genetics, metabolomics, bioinformatics, and molecular biology associated with targeting virulent factors of diseases. In addition, he has studied at Augusta University (formerly, Georgia Regents University) in Augusta, GA, USA, on a J-1 Exchange Scholar Visa and at Ben-Gurion University, Israel. He is also a life member fellow in the Indian Science Congress and the Agriculture, Nutrition and Health Academy, UK. Dr. Anbarasu Kannan is a senior scientist and assistant professor in the Department of Biochemistry, CSIR-CFTRI, Mysuru, Karnataka, India. He had previous training in both Doctorate (2013) and post-doctoral (from 2014-2017) in cancer research. During post-doctoral training, he has been working on exosome biogenesis, mitochondrial genetic alterations, and dynamics in different cancers. His work has been published in peer-reviewed journals. He has been awarded the Early career Research Award (ECRA) in tracing the alteration signature of SH3GL2 in circulating exosomes of breast cancer progression to develop exosome-based biomarker. He has research expertise in the field of exosome biogenesis and mitochondrial dynamics, and in development of exosome biomarkers for cancer diagnostics and therapeutics.Dr. Deepjyoti Singh is a Postdoctoral Researcher at the Department of Entomology and Plant Pathology, North Carolina State University, Raleigh, North Carolina, USA. With over a decade of expertise in molecular biology, her work focuses on plant-virus-vector interactions. She earned her Ph.D. from the School of Life Sciences, Jawaharlal Nehru University, New Delhi, India, where she studied the mechanism of systemic acquired resistance in plants. During her tenure as a postdoctoral fellow at Syracuse University, New York, USA, she worked on understanding the epigenetic mechanism of plant defense in Arabidopsis. She has published 8 research articles in reputable international journals and presented her work at various national and international conferences. In addition to her research, Dr. Singh contributes as a reviewer for esteemed journals, including Molecular Plant-Microbe Interactions (MPMI) and Frontiers in Microbiology.Dr. Sunil L is currently working as a postdoctoral fellow at the Department of Pharmacological and Pharmaceutical Sciences at the University of Houston College of Pharmacy, Houston, TX, USA. He mainly focuses on small molecules of the Munc13.1.C1 domain and the Munc13.1.C1.C2B domain of presynaptic CNS protein, intending to develop drugs for alcohol addiction and Alzheimer’s disease. His research focuses on protein expression, purification, characterization, and molecular simulation of Munc13.1 presynaptic protein and its interaction with small molecules. He has published 22 research articles in Web of Science indexed journals.

• Section 1 Extracellular vesicles/exosomes as novel sources of cellular communication1. Historical context and the evolution of exosome research2. The cellular machinery behind exosome formation3. Exosomes as messengers: intercellular communication4. Exosomes: essential messengers in intercellular communication5. Exosomes as cellular couriers: the mechanisms of intercellular messagingSection 2 Extracellular vesicles/exosomes: methods and protocols6. Comprehensive approaches to isolation, characterization, and functional assessment of extracellular vesiclesSection 3 Extracellular vesicles/exosomes in health and diseases: Potential applications7. Exosomes as biomarkers for disease diagnosis8. The intricate interplay between cancer cells and exosomes9. Advancements in stem cell therapy facilitated by exosomes10. Crossing the blood–brain barrier: exosomes in neurological research11. Exosomes as vectors across the blood–brain barrier in neurological research12. Unraveling the role of exosomes in modulating immune responses13. The immunological impact of exosomes: modulation and mechanisms14. Exploring the link between exosomes and metabolic diseases15. Role of exosomal microRNAs in anticancer drug resistance and tumor angiogenesis16. Health implications of retinoic acid in exosome biogenesis, secretion, and uptake17. Exosomes in aging and longevitySection 4 Engineering and scaling up extracellular vesicles and exosomes production: Medicine, nutraceuticals, cosmetics, and novel applications18. Cutting-edge technology for extracellular vesicles and exosomes production: future prospects19. Novel application of extracellular vesicles and exosomes in nutraceutical and cosmetic production20. Ethical considerations, challenges, and the road ahead: exosomes in the next decade

Dr. Chandrashekhar Singh is a Research Associate at the Department of Pharmaceutical Engineering and Technology, Indian Institute of Technology, Banaras Hindu University, Varanasi, Uttar Pradesh, India. Dr. Singh earned his Ph.D. in Biotechnology from the Department of Botany, Banaras Hindu University. Dr. Singh has authored 19 research articles that have been published in reputable international journals. Additionally, he has written 4 book chapters in collaboration with renowned science publishers. Dr. Singh's primary research focuses on the development of bio-inspired nanoparticles and their applications as antibacterial in the management of wound infections and plant Biotechnology. His contributions to this field have earned him recognition, including an Indian patent. Furthermore, Dr. Singh is actively engaged in the preparation of more research and review articles, which are currently in the process of publication. Dr. Singh also contributes to the academic community as a reviewer for esteemed journals such as Vegetos (Springer). Dr. Akhileshwar Kumar Srivastava works as a Research Associate (ICMR) at CSIR-Central Food Technological Research Institute, Mysore, India. In his 11 years of research, he has published more than 18 research articles in reputed international journals, along with 4 books and 11 book chapters. His research specialization is primarily in the area of pharmacognosy with genetics, metabolomics, bioinformatics, and molecular biology associated with targeting virulent factors of diseases. In addition, he has studied at Augusta University (formerly, Georgia Regents University) in Augusta, GA, USA, on a J-1 Exchange Scholar Visa and at Ben-Gurion University, Israel. He is also a life member fellow in the Indian Science Congress and the Agriculture, Nutrition and Health Academy, UK. Dr. Anbarasu Kannan is a senior scientist and assistant professor in the Department of Biochemistry, CSIR-CFTRI, Mysuru, Karnataka, India. He had previous training in both Doctorate (2013) and post-doctoral (from 2014-2017) in cancer research. During post-doctoral training, he has been working on exosome biogenesis, mitochondrial genetic alterations, and dynamics in different cancers. His work has been published in peer-reviewed journals. He has been awarded the Early career Research Award (ECRA) in tracing the alteration signature of SH3GL2 in circulating exosomes of breast cancer progression to develop exosome-based biomarker. He has research expertise in the field of exosome biogenesis and mitochondrial dynamics, and in development of exosome biomarkers for cancer diagnostics and therapeutics.Dr. Deepjyoti Singh is a Postdoctoral Researcher at the Department of Entomology and Plant Pathology, North Carolina State University, Raleigh, North Carolina, USA. With over a decade of expertise in molecular biology, her work focuses on plant-virus-vector interactions. She earned her Ph.D. from the School of Life Sciences, Jawaharlal Nehru University, New Delhi, India, where she studied the mechanism of systemic acquired resistance in plants. During her tenure as a postdoctoral fellow at Syracuse University, New York, USA, she worked on understanding the epigenetic mechanism of plant defense in Arabidopsis. She has published 8 research articles in reputable international journals and presented her work at various national and international conferences. In addition to her research, Dr. Singh contributes as a reviewer for esteemed journals, including Molecular Plant-Microbe Interactions (MPMI) and Frontiers in Microbiology.Dr. Sunil L is currently working as a postdoctoral fellow at the Department of Pharmacological and Pharmaceutical Sciences at the University of Houston College of Pharmacy, Houston, TX, USA. He mainly focuses on small molecules of the Munc13.1.C1 domain and the Munc13.1.C1.C2B domain of presynaptic CNS protein, intending to develop drugs for alcohol addiction and Alzheimer’s disease. His research focuses on protein expression, purification, characterization, and molecular simulation of Munc13.1 presynaptic protein and its interaction with small molecules. He has published 22 research articles in Web of Science indexed journals.

• Section 1 Extracellular vesicles/exosomes as novel sources of cellular communication1. Historical context and the evolution of exosome research2. The cellular machinery behind exosome formation3. Exosomes as messengers: intercellular communication4. Exosomes: essential messengers in intercellular communication5. Exosomes as cellular couriers: the mechanisms of intercellular messagingSection 2 Extracellular vesicles/exosomes: methods and protocols6. Comprehensive approaches to isolation, characterization, and functional assessment of extracellular vesiclesSection 3 Extracellular vesicles/exosomes in health and diseases: Potential applications7. Exosomes as biomarkers for disease diagnosis8. The intricate interplay between cancer cells and exosomes9. Advancements in stem cell therapy facilitated by exosomes10. Crossing the blood–brain barrier: exosomes in neurological research11. Exosomes as vectors across the blood–brain barrier in neurological research12. Unraveling the role of exosomes in modulating immune responses13. The immunological impact of exosomes: modulation and mechanisms14. Exploring the link between exosomes and metabolic diseases15. Role of exosomal microRNAs in anticancer drug resistance and tumor angiogenesis16. Health implications of retinoic acid in exosome biogenesis, secretion, and uptake17. Exosomes in aging and longevitySection 4 Engineering and scaling up extracellular vesicles and exosomes production: Medicine, nutraceuticals, cosmetics, and novel applications18. Cutting-edge technology for extracellular vesicles and exosomes production: future prospects19. Novel application of extracellular vesicles and exosomes in nutraceutical and cosmetic production20. Ethical considerations, challenges, and the road ahead: exosomes in the next decade