Transketolases

Claude Bernard Lyon 1 and ICBMS (Institut de Chimie et Biochimie Moléculaires et Supramoléculaires, UMR 5246 CNRS, France).After biochemistry and biology studies in Rouen (France), he obtained a master degree in Amiens on a biocatalysis topic, followed by a PhD at the RWTH-Aachen university (Germany) working on immobilized enzymes in two-phase systems under Prof. W. Hartemeier supervision. He joined the group of Dr. Rolland Furstoss and Dr. Alain Archelas at the CNRS in Marseille (France) for a post-doc on multi-gram scale biocatalysis using epoxide hydrolases. After a two-years temporary associate professor position at the university of Cergy-Pontoise (France) with Prof. Véronique Larreta-Garde on sol-gel transitions of protein-polysaccharides mixtures catalysed by enzymes, he became a lecturer at the University Lyon 1in 2006 where he developed original research in heterogeneous biocatalysis (ionic liquids) and more recently electrochemical screening assays using screen-printed electrodes for oxidoreductases (dehydrogenases, oxidases, laccases) and transketolases in collaboration with Dr. Franck Charmantray. Franck CHARMANTRAY is a researcher in Biocatalysis at the CNRS. He carries out his research work at the Institute of Chemistry in Clermont-Ferrand (ICCF, UMR6296, Clermont Auvergne University, France). In 2001, he obtained a PhD in medicinal chemistry focusing on « A new family of DNA intercalating-alkylating agents » under the supervision of Dr. M. Demuynck, Grenoble 1 University, France. He then changed his research theme to focus on Studies on Homocitrate Synthase and other Lysine Pathway Enzymes during a postdoctoral internship funded by a BBRSC Fellowship, and carried out under the supervision of Prof. D. Young, at the University of Sussex, Brighton, UK. He returned to France to carry out a 2-year industrial postdoctoral internship in Biocatalysis financed by Laboratoires Fournier and which focused on the chemoenzymatic synthesis of antithrombotics. He was then recruited at the ICCF as a CNRS researcher in 2004. His research themes concern Biocatalysis, and in particular the study and optimization of transketolase by in vitro evolution for the synthesis of rare sugars and analogues in particular. He works in close collaboration with Dr. Bastien Doumèche with whom he recently developed an electrochemical screening for microbial transketolase inhibitors identification.

• 1. Role of transketolase-like 1 in human cortical neurogenesisAnneline Pinson and Wieland B. Huttner2. Transketolase in metabolic diseases, autoimmune diseases and cancerXuemei Tong3. Transketolase-like-1 (TKTL1) based one-substrate reaction and its impact on pathologies and treatmentsJohannes Coy4. Evaluation of binding, affinity and metabolic regulatory activity of a transketolase inhibitorXiaofei Chen5. Discovery of novel transketolase epitopes and development of IgG-based tuberculosis serodiagnosticsLobelia Samavati6. Transketolases in apicomplexan parasitesNishith Gupta7. Electrochemical screening for microbial transketolase inhibitors identificationChloé Aymard, Bastien Doumèche, Florian Bianco, Jean-François Chateaux, Franck Charmantray and Rainier-Numa Georges8. Transketolases isolation from plants: Diversity of pathwaysDorothea Bartels9. Transketolase-sulfoglycolytic pathwayYan Zhang 10. Split-gene transketolase homolog that catalyzes the key step of biosynthetic pathway of phosphonothrixin in Saccharothrix sp.Tomohisa Kuzuyama and Yunxun Zhu11. Biophysical and structural analysis of transketolasesKai Tittman and Fabian Rabe von Pappenheim12. Structural variations of transketolases over the evolution from bacteria to higher eukaryotes: in silico/in vitro studyRainier-Numa Georges, Bastien Doumèche, Lionel Ballut and Franck Charmantray13. Structural and biochemical studies on split-gene transketolases, evolutionary aspects and comparisonsJennifer Ann Littlechild14. Transketolase catalytic reaction pathway. Study of ThDP activation and complete catalytic cycleVincent Thery and Lionel Nauton15. Transketolases: Theoretical aspects from QM and QM/MM investigationsTiziana Marino, Mario Prejanò and Nino Russo16. Computational and structural insights into transketolase function and evolution: A detailed methodologyThommas Mutemi Musyoka and Rita Afriyie Boateng17. Transketolase enzyme discovery and the enzyme evolution of transketolaseAlessia Tonoli, Helen C. Hailes, Paul Dalby and Jack Jeffries18. Evolved thermostable transketolase from Geobacillus stearothermophilus for deoxyketoses synthesisCamille Gadona, Franck Charmantray, Laurence Hecquet, Giuseppe Arbia and Muriel Joly19. Practical screening assays to identify the substrate scope of transketolase variantsWolf-Dieter Fessner and Jules Perrard20. Production of rare sugars by transketolase in combination with microbes and other enzymesAkihide Yoshihara, Yoshida Hiromi, Ken Izumori and Shigehiro Kamitori21. Engineered transketolase for cascade enzymatic synthesis with w-transaminaseShuangjun Lin22. Process considerations for the scale-up and implementation of transketolasesJohn Woodley