Bioorthogonal Labeling

After completing studies for the A.B., A.M., and Ph.D. degrees in chemistry at Harvard University, David W. Christianson joined the faculty of the University of Pennsylvania, where he is currently the Roy and Diana Vagelos Professor in Chemistry and Chemical Biology. At Penn, Christianson’s research focuses on the structural and chemical biology of the zinc-dependent histone deacetylases as well as enzymes of terpene biosynthesis. His research accomplishments have been recognized by several awards, including the Pfizer Award in Enzyme Chemistry and the Repligen Award in Chemistry of Biological Processes from the American Chemical Society, a Guggenheim Fellowship, and the Elizabeth S. and Richard M. Cashin Fellowship from the Radcliffe Institute for Advanced Study at Harvard University. Christianson is also a dedicated classroom teacher, and his accomplishments in this regard have been recognized by the Lindback Award for Distinguished Teaching at Penn and a Rhodes Trust Inspirational Educator Award from Oxford University. Christianson has also held visiting professorships in the Department of Biochemistry at Cambridge University and the Department of Chemistry and Chemical Biology at Harvard University. Christianson has served with Prof. Anna Pyle as Co-Editor-in-Chief of Methods in Enzymology since 2015.Dr. Karen N. Allen works at the Department of Chemistry of the Boston University, the Metcalf Center for Science and Engineering

• 1. Staudinger Ligations and Related Reactions: Scope and Applications in Chemical BiologyStefan Bräse, Juliane Ott and Ute Schepers2. Strain-Promoted Bioorthogonal Cycloadditions to Investigate Sialyltransferases Promiscuity Towards Unnatural Sialic AcidsFrederic Friscourt and Zoeisha Chinoy3. Practical Guidelines for the Bioorthogonal AMI–Isonitrile LigationHelma Wennemers4. Nitrones and Other Dipoles in Bioorthogonal Chemistry ApplicationsJohn Paul Pezacki5. 1,2-Aminothiol-Specific Conjugation for Dual-Color Fluorescent Labeling via Ultrafast TAMMsYu-Hsuan Tsai, Linlin Wang, Gaocan Dai and Sicong Yao6. Genetic Incorporation of Bicyclononyne for Cell-Surface Protein Labeling via Tetrazine LigationYu-Hsuan Tsai, Yawei Liu, Lei Zhang and Sicong Yao7. Genetic Code Expansion for Single Noncanonical Amino Acid Incorporation into SARS-CoV-2 Omicron Spike on Amber-Free Lentiviral ParticlesMaoliln Lu8. Bioorthogonal Click-Chemistry Labeling and Visualization of Dual Noncanonical Amino Acid–Incorporated HIV-1 Env on Intact VirionsMaoliln Lu9. Studying Bacterial Glycans with Azidosugars and Bioorthogonal ChemistryDanielle H. Dube, Aniqa Chowdhury, Divya Bhargava and Karen Moulton10. Bioorthogonal Tagging of DNA and RNASeergazhi G. Srivatsan11. Efficient Labeling of Pathogenic Fungal Chitin with Bioorthogonal ProbesCatherine L. Grimes12. Dendritic Cell Metabolic Labeling and TargetingHua Wang13. Orthogonal Crosslinking of Monobodies Using a Genetically Encoded β-Lactam Amino AcidQing Lin14. Bioorthogonal Chemical Labeling of Endogenous Receptors in the Live Mouse BrainItaru Hamachi15. Organelle-Directed Metabolic Glycan LabelingShou-Fa Han, Shixiong Wen, Yilong Shi, Enkang Zhang and Jiahuai Han16. Bioorthogonal Click Labeling of Proteins for Molecular Resolution Fluorescence ImagingMarkus Sauer17. Molecular Engineering of Activatable Cyclopropenes for Bioorthogonal LabelingScott T. Laughlin18. Pyrone and Pyranthione Scaffolds for Click-to-Release Biological Gas DeliveryScott T. Laughlin19. Click and Release through Bioorthogonal HydroaminationJustin Kim, Tyler J Brown and Yiming Guo20. Site-Specific Bioorthogonal Labeling of AntibodiesLai-Xi Wang21. inCu-click: Enabling Copper-Catalyzed Click Chemistry Inside Living CellsSara H. Rouhanifard22. Bioorthogonal Photocatalytic Proximity Labeling for Quantitative Mapping of Cell–Cell InteractionsXinyuan Fan, Yan Zhang, Nan Zhou and Fuhu Guo23. Bioorthogonal Labeling of Sialic Acid Isomers for Imaging of Glycoconjugates Using Mass Spectrometry and MicroscopyRichard R. Drake