Plant Tissue Culture

Plant Tissue Culture, Fifth Edition offers a practice-driven introduction to one of the most influential experimental platforms in plant science. Combining established methodologies with contemporary advances in biotechnology, it demonstrates how cultured cells, tissues, and organs can be used to explore plant development and morphogenesis, propagate valuable germplasm, generate novel variation, and support crop trait enhancement.

Structured around a series of laboratory exercises, the book enables technical proficiency alongside an in-depth understanding of the biological principles that govern cellular growth, function, and regenerative responses. Readers are guided through the investigative techniques that have shaped the field, from micropropagation and embryo rescue to protoplast technologies and plant transformation, illustrating how in-vitro systems continue to serve as powerful tools for both discovery and application.

This fully revised edition also discusses the expanding relationship between culture-based methods and molecular approaches, including genome editing and RNA interference. Building on decades of established principles and placing cutting-edge developments in the broader context of plant research and agricultural innovation, the volume highlights the continuing evolution of a discipline that remains central to unlocking and harnessing plant potential.

• Provides step-by-step guidance for establishing and maintaining successful culture systems, helping ensure reproducible laboratory outcomes
• Illustrates the mechanisms underlying plant regeneration, facilitating the effective implementation of culture techniques across diverse species and biological questions
• Examines specialized protocols such as anther culture, meristem culture, protoplast fusion, and gene transformation, broadening the toolkit available for breeding, genetic modification, and the production of disease-free planting material
• Highlights the contribution of tissue culture to commercial micropropagation and the study of plant-derived metabolites, attesting to its versatility across both scientific and industrial settings