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2 produkter
2 produkter
Häftad, Engelska, 2021
1 161 kr
Skickas inom 10-15 vardagar
Plant Tissue Culture: Techniques and Experiments, Fourth Edition, builds on the classroom tested, audience proven manual that has guided users through successful plant culturing for almost 30 years. The book's experiments demonstrate major concepts and can be conducted with a variety of plant materials readily available throughout the year. This fully updated edition describes the principles of the newest technologies, including CRISPR/Cas9 gene editing and RNAi technology with plant cell and tissue cultures and their applications. Bridging the gap between theory and practice, this book contains detailed methodology supported by comprehensive illustrations, giving users a diverse learning experience for both university students and plant scientists. Provides fundamental principles, methods and techniques in plant cell, tissue and organ culture that can be applied to all crop plants, including agronomic crops, horticulture and forestry crops for germplasm improvement Guides readers from lab setup to supplies, stock solution and media preparation, explant selection and disinfestations, and experimental observations and measurement Contains the latest advances and updates since the previous edition published in 2012
Häftad, Engelska, 2027
1 426 kr
Kommande
Plant Tissue Culture 5e, is a rigorously updated edition that serves as a critical reference for advanced research in plant biotechnology and tissue culture methodologies. This comprehensive volume provides an in-depth exploration of essential techniques, from the preparation of molar solutions and the dissolution of kinetins in stock solutions to the application of cutting-edge CRISPR/Cas9 and RNA interference (RNAi) technologies. This edition features a series of experimental protocols that explore key principles of plant tissue culture, utilizing a diverse array of plant materials. Each experiment is designed to reinforce theoretical concepts while providing practical applications relevant to contemporary research. The text thoroughly addresses the underlying mechanisms of gene editing and RNAi, detailing their integration into plant cell and tissue culture systems. As advancements in sequencing technologies have exponentially increased the availability of plant genomic data, this book emphasizes the significance of functional genome analysis and genetic transformation processes. It explores how these methodologies can be employed to investigate the relationship between genome sequences and phenotypic expression, ultimately contributing to the enhancement of crop traits. Key topics include the design and optimization of sgRNAs for Cas9 and Cas12, the exploration of alternative Cas platforms, and the methodologies for gene editing detection, alongside an examination of plant secondary metabolites and their roles in plant physiology. For over 30 years, *Plant Tissue Culture* has been an authoritative resource for researchers and students in plant sciences, providing the essential knowledge and techniques necessary to advance laboratory practices and deepen the understanding of plant cellular structures and functions.Fully revised and updated throughout, ensuring latest techniques and practices are includedProvides fundamental principles, methods and techniques in plant cell, tissue and organ culture that can be applied to all crop plants, including agronomic crops, horticulture and forestry crops for germplasm improvementGuides readers from lab setup to supplies, stock solution and media preparation, explant selection and disinfestations, and experimental observations and measurement