After completing studies for the A.B., A.M., and Ph.D. degrees in chemistry at Harvard University, David W. Christianson joined the faculty of the University of Pennsylvania, where he is currently the Roy and Diana Vagelos Professor in Chemistry and Chemical Biology. At Penn, Christianson’s research focuses on the structural and chemical biology of the zinc-dependent histone deacetylases as well as enzymes of terpene biosynthesis. His research accomplishments have been recognized by several awards, including the Pfizer Award in Enzyme Chemistry and the Repligen Award in Chemistry of Biological Processes from the American Chemical Society, a Guggenheim Fellowship, and the Elizabeth S. and Richard M. Cashin Fellowship from the Radcliffe Institute for Advanced Study at Harvard University. Christianson is also a dedicated classroom teacher, and his accomplishments in this regard have been recognized by the Lindback Award for Distinguished Teaching at Penn and a Rhodes Trust Inspirational Educator Award from Oxford University. Christianson has also held visiting professorships in the Department of Biochemistry at Cambridge University and the Department of Chemistry and Chemical Biology at Harvard University. Christianson has served with Prof. Anna Pyle as Co-Editor-in-Chief of Methods in Enzymology since 2015.Dr. Karen N. Allen works at the Department of Chemistry of the Boston University, the Metcalf Center for Science and Engineering Subashchandrabose Chinnathambi is Additional Professor at the Department of Neurochemistry, National Institute of Mental Health and Neurosciences Hospital Bangalore, Karnataka, India. His scientific interests include physiopathology of neurodegenerative disorders, Tau Cytoskeleton, Tau-GPCR, purinergic and chemokine receptors, Tau stem cells, animal models, neuropharmacology, therapeutic approaches targeting Tau oligomers, and biomarkers for neurodegeneration. He serves on the editorial board for various international cell biology journals and is also a regular invited speaker at a number of meetings and workshops. Dr. Chinnathambi has more than 16 years of research experience in the area of neuroscience and Tau-related research in Alzheimer’s Disease.

• 1. Accurate prediction of RNA-binding amino acids in protein sequences with hybridRNAbindLukasz Kurgan2. Direct neuronal reprogramming by transdifferentiation and application of induced neuronsSubashchandrabose Chinnathambi, Vaishnavi Anathanarayana and Tanu Suma Narayanappa3. AGO2-Dependent Non-Canonical Interaction of miR-532-3p Mediates Regulation of Nonmuscle myosin IICSiddhartha S. Jana, Kumarjeet Banerjee, Shekhar Saha and Debapriya Dey4. Method of following RNA and Protein Cargo trafficking to and out of phase-separated RNA processing bodies in mammalian cellsSuvendra Bhattacharyya and Kamalika Mukherjee5. Comprehensive Structural Analysis of Thrombectomy Samples Using Complementary Fixation and Staining MethodsGhulam Jeelani Pir and Aijaz Parray6. Methods to Analyze Semi-Extractable RNAs and Their Interactions with Long non-coding RNAs and ProteinsAshish Misra7. Enzymatic Incorporation of a Dual-Functional Nucleotide Probe for Antibiotic-Induced Conformational Changes in the Bacterial Ribosomal RNASeergazhi G. Srivatsan and Saddam Khatik8. Characterization of alpha-synuclein phase separation in cellsSamir K. Maji, Riya Bera, Ranjit Shaw and Arunima Sakunthala9. Fluorescence Bimolecular Complementation Enables Facile Detection of Ribosome Assembly Defects in Escherichia coliAnand Baskaran10. A Method for Identification of RNA Targets of RNA Binding Protein by EditingBaskar Bakthavachalu, Omkar Koppaka and Nitish Rawat11. RNA and the RNA-binding protein FUS act in concert to prevent TDP-43 spatial segregationLoic Hamon12. Methodologies for Deciphering TDP-43 Proteinopathy: From Genetic Modifiers to Aggregate ProteomicsMousumi Mutsuddi, Pranjali Pandey and Ashim Mukherjee13. RNA-protein interactions and its complexesIndranil Malik14. RNA-binding domains drive the double-stranded RNA recognition and bindingJeetender Chugh15. A proximity ligation assay (PLA)-based method for detecting the interaction of RNA-binding proteins with mRNAs in cellsPartho Sarothi Ray16. Arginine methylation and cytoplasmic mRNA fatePurusharth Rajyaguru, Sakshi Dewasthale and Gayatri Mohanan17. Tah1, a key component of R2TP complex that regulates assembly of snoRNPDeepak Kumar Sharma18. Back Splice Junction Based Methods for Circular RNAs Detection and EnrichmentSrivatsava Naidu and Deepika Antil19. Suppressor mutation screening to understand the molecular mechanisms of RNA-protein interaction in translationPankaj V. Alone, Charles Antony and Tanaya Kole20. RNA based therapeutics in Inflammation Model NSCLCShailza Singh, Pooja Gulhane, Manasi Tukrul and Swati Goswami21. Non-coding RNA-RBP complexes in Epigenetic Regulation during Megakaryocyte DevelopmentRavi Kumar Gutti22. Methods of regulated and cooperative miRNA packaging into the endosomal lumen by miRNA-binding protein HuRSuvendra Bhattacharyya and Kamalika Mukherjee23. Biophysical characterization of p53 tetramerization domain mutant R337CAbdul Samath Ethayathulla

After completing studies for the A.B., A.M., and Ph.D. degrees in chemistry at Harvard University, David W. Christianson joined the faculty of the University of Pennsylvania, where he is currently the Roy and Diana Vagelos Professor in Chemistry and Chemical Biology. At Penn, Christianson’s research focuses on the structural and chemical biology of the zinc-dependent histone deacetylases as well as enzymes of terpene biosynthesis. His research accomplishments have been recognized by several awards, including the Pfizer Award in Enzyme Chemistry and the Repligen Award in Chemistry of Biological Processes from the American Chemical Society, a Guggenheim Fellowship, and the Elizabeth S. and Richard M. Cashin Fellowship from the Radcliffe Institute for Advanced Study at Harvard University. Christianson is also a dedicated classroom teacher, and his accomplishments in this regard have been recognized by the Lindback Award for Distinguished Teaching at Penn and a Rhodes Trust Inspirational Educator Award from Oxford University. Christianson has also held visiting professorships in the Department of Biochemistry at Cambridge University and the Department of Chemistry and Chemical Biology at Harvard University. Christianson has served with Prof. Anna Pyle as Co-Editor-in-Chief of Methods in Enzymology since 2015.Dr. Karen N. Allen works at the Department of Chemistry of the Boston University, the Metcalf Center for Science and Engineering Subashchandrabose Chinnathambi is Additional Professor at the Department of Neurochemistry, National Institute of Mental Health and Neurosciences Hospital Bangalore, Karnataka, India. His scientific interests include physiopathology of neurodegenerative disorders, Tau Cytoskeleton, Tau-GPCR, purinergic and chemokine receptors, Tau stem cells, animal models, neuropharmacology, therapeutic approaches targeting Tau oligomers, and biomarkers for neurodegeneration. He serves on the editorial board for various international cell biology journals and is also a regular invited speaker at a number of meetings and workshops. Dr. Chinnathambi has more than 16 years of research experience in the area of neuroscience and Tau-related research in Alzheimer’s Disease.

• 1. Accurate prediction of RNA-binding amino acids in protein sequences with hybridRNAbindLukasz Kurgan2. Direct neuronal reprogramming by transdifferentiation and application of induced neuronsSubashchandrabose Chinnathambi, Vaishnavi Anathanarayana and Tanu Suma Narayanappa3. AGO2-Dependent Non-Canonical Interaction of miR-532-3p Mediates Regulation of Nonmuscle myosin IICSiddhartha S. Jana, Kumarjeet Banerjee, Shekhar Saha and Debapriya Dey4. Method of following RNA and Protein Cargo trafficking to and out of phase-separated RNA processing bodies in mammalian cellsSuvendra Bhattacharyya and Kamalika Mukherjee5. Comprehensive Structural Analysis of Thrombectomy Samples Using Complementary Fixation and Staining MethodsGhulam Jeelani Pir and Aijaz Parray6. Methods to Analyze Semi-Extractable RNAs and Their Interactions with Long non-coding RNAs and ProteinsAshish Misra7. Enzymatic Incorporation of a Dual-Functional Nucleotide Probe for Antibiotic-Induced Conformational Changes in the Bacterial Ribosomal RNASeergazhi G. Srivatsan and Saddam Khatik8. Characterization of alpha-synuclein phase separation in cellsSamir K. Maji, Riya Bera, Ranjit Shaw and Arunima Sakunthala9. Fluorescence Bimolecular Complementation Enables Facile Detection of Ribosome Assembly Defects in Escherichia coliAnand Baskaran10. A Method for Identification of RNA Targets of RNA Binding Protein by EditingBaskar Bakthavachalu, Omkar Koppaka and Nitish Rawat11. RNA and the RNA-binding protein FUS act in concert to prevent TDP-43 spatial segregationLoic Hamon12. Methodologies for Deciphering TDP-43 Proteinopathy: From Genetic Modifiers to Aggregate ProteomicsMousumi Mutsuddi, Pranjali Pandey and Ashim Mukherjee13. RNA-protein interactions and its complexesIndranil Malik14. RNA-binding domains drive the double-stranded RNA recognition and bindingJeetender Chugh15. A proximity ligation assay (PLA)-based method for detecting the interaction of RNA-binding proteins with mRNAs in cellsPartho Sarothi Ray16. Arginine methylation and cytoplasmic mRNA fatePurusharth Rajyaguru, Sakshi Dewasthale and Gayatri Mohanan17. Tah1, a key component of R2TP complex that regulates assembly of snoRNPDeepak Kumar Sharma18. Back Splice Junction Based Methods for Circular RNAs Detection and EnrichmentSrivatsava Naidu and Deepika Antil19. Suppressor mutation screening to understand the molecular mechanisms of RNA-protein interaction in translationPankaj V. Alone, Charles Antony and Tanaya Kole20. RNA based therapeutics in Inflammation Model NSCLCShailza Singh, Pooja Gulhane, Manasi Tukrul and Swati Goswami21. Non-coding RNA-RBP complexes in Epigenetic Regulation during Megakaryocyte DevelopmentRavi Kumar Gutti22. Methods of regulated and cooperative miRNA packaging into the endosomal lumen by miRNA-binding protein HuRSuvendra Bhattacharyya and Kamalika Mukherjee23. Biophysical characterization of p53 tetramerization domain mutant R337CAbdul Samath Ethayathulla

After completing studies for the A.B., A.M., and Ph.D. degrees in chemistry at Harvard University, David W. Christianson joined the faculty of the University of Pennsylvania, where he is currently the Roy and Diana Vagelos Professor in Chemistry and Chemical Biology. At Penn, Christianson’s research focuses on the structural and chemical biology of the zinc-dependent histone deacetylases as well as enzymes of terpene biosynthesis. His research accomplishments have been recognized by several awards, including the Pfizer Award in Enzyme Chemistry and the Repligen Award in Chemistry of Biological Processes from the American Chemical Society, a Guggenheim Fellowship, and the Elizabeth S. and Richard M. Cashin Fellowship from the Radcliffe Institute for Advanced Study at Harvard University. Christianson is also a dedicated classroom teacher, and his accomplishments in this regard have been recognized by the Lindback Award for Distinguished Teaching at Penn and a Rhodes Trust Inspirational Educator Award from Oxford University. Christianson has also held visiting professorships in the Department of Biochemistry at Cambridge University and the Department of Chemistry and Chemical Biology at Harvard University. Christianson has served with Prof. Anna Pyle as Co-Editor-in-Chief of Methods in Enzymology since 2015.Dr. Karen N. Allen works at the Department of Chemistry of the Boston University, the Metcalf Center for Science and Engineering Subashchandrabose Chinnathambi is Additional Professor at the Department of Neurochemistry, National Institute of Mental Health and Neurosciences Hospital Bangalore, Karnataka, India. His scientific interests include physiopathology of neurodegenerative disorders, Tau Cytoskeleton, Tau-GPCR, purinergic and chemokine receptors, Tau stem cells, animal models, neuropharmacology, therapeutic approaches targeting Tau oligomers, and biomarkers for neurodegeneration. He serves on the editorial board for various international cell biology journals and is also a regular invited speaker at a number of meetings and workshops. Dr. Chinnathambi has more than 16 years of research experience in the area of neuroscience and Tau-related research in Alzheimer’s Disease.

• 1. Accurate prediction of RNA-binding amino acids in protein sequences with hybridRNAbindLukasz Kurgan2. Direct neuronal reprogramming by transdifferentiation and application of induced neuronsSubashchandrabose Chinnathambi, Vaishnavi Anathanarayana and Tanu Suma Narayanappa3. AGO2-Dependent Non-Canonical Interaction of miR-532-3p Mediates Regulation of Nonmuscle myosin IICSiddhartha S. Jana, Kumarjeet Banerjee, Shekhar Saha and Debapriya Dey4. Method of following RNA and Protein Cargo trafficking to and out of phase-separated RNA processing bodies in mammalian cellsSuvendra Bhattacharyya and Kamalika Mukherjee5. Comprehensive Structural Analysis of Thrombectomy Samples Using Complementary Fixation and Staining MethodsGhulam Jeelani Pir and Aijaz Parray6. Methods to Analyze Semi-Extractable RNAs and Their Interactions with Long non-coding RNAs and ProteinsAshish Misra7. Enzymatic Incorporation of a Dual-Functional Nucleotide Probe for Antibiotic-Induced Conformational Changes in the Bacterial Ribosomal RNASeergazhi G. Srivatsan and Saddam Khatik8. Characterization of alpha-synuclein phase separation in cellsSamir K. Maji, Riya Bera, Ranjit Shaw and Arunima Sakunthala9. Fluorescence Bimolecular Complementation Enables Facile Detection of Ribosome Assembly Defects in Escherichia coliAnand Baskaran10. A Method for Identification of RNA Targets of RNA Binding Protein by EditingBaskar Bakthavachalu, Omkar Koppaka and Nitish Rawat11. RNA and the RNA-binding protein FUS act in concert to prevent TDP-43 spatial segregationLoic Hamon12. Methodologies for Deciphering TDP-43 Proteinopathy: From Genetic Modifiers to Aggregate ProteomicsMousumi Mutsuddi, Pranjali Pandey and Ashim Mukherjee13. RNA-protein interactions and its complexesIndranil Malik14. RNA-binding domains drive the double-stranded RNA recognition and bindingJeetender Chugh15. A proximity ligation assay (PLA)-based method for detecting the interaction of RNA-binding proteins with mRNAs in cellsPartho Sarothi Ray16. Arginine methylation and cytoplasmic mRNA fatePurusharth Rajyaguru, Sakshi Dewasthale and Gayatri Mohanan17. Tah1, a key component of R2TP complex that regulates assembly of snoRNPDeepak Kumar Sharma18. Back Splice Junction Based Methods for Circular RNAs Detection and EnrichmentSrivatsava Naidu and Deepika Antil19. Suppressor mutation screening to understand the molecular mechanisms of RNA-protein interaction in translationPankaj V. Alone, Charles Antony and Tanaya Kole20. RNA based therapeutics in Inflammation Model NSCLCShailza Singh, Pooja Gulhane, Manasi Tukrul and Swati Goswami21. Non-coding RNA-RBP complexes in Epigenetic Regulation during Megakaryocyte DevelopmentRavi Kumar Gutti22. Methods of regulated and cooperative miRNA packaging into the endosomal lumen by miRNA-binding protein HuRSuvendra Bhattacharyya and Kamalika Mukherjee23. Biophysical characterization of p53 tetramerization domain mutant R337CAbdul Samath Ethayathulla