RNA-binding Proteins Part C

This volume of Methods in Enzymology presents a practical and multidisciplinary collection of contemporary methods for investigating RNA biology, RNA-binding proteins (RBPs), RNA-protein interactions, ribonucleoprotein (RNP) complexes, biomolecular condensates, liquid-liquid phase separation, RNA processing, translation, and RNA-mediated regulation. The chapters integrate complementary experimental and computational approaches, including biochemical reconstitution, quantitative microscopy, transcriptomics, proteomics, interactomics, NMR spectroscopy, structural biology, computational analysis, and tissue-based techniques.

Together, these methods provide a versatile framework for elucidating how RNA and RBPs regulate cellular organization, gene expression, RNA metabolism, RNA processing, translation, and biomolecular phase behavior. Particular emphasis is placed on emerging applications in disease-associated molecular pathways, including neurodegeneration and neurological disorders, with coverage of topics relevant to Alzheimer’s disease, Tau biology, and HIV-associated neurocognitive disorders. This volume provides researchers with practical methodological strategies for dissecting RNA-protein interactions and defining RBP function across molecular, cellular, and disease contexts. The collection is designed to serve as a valuable methodological resource for researchers investigating the mechanisms by which RNA-protein networks govern cellular processes and contribute to human disease.

• RNA biology and RNA-binding proteins (RBPs)
• RNA-protein interactions
• Biomolecular condensates
• RNA-dependent liquid-liquid phase separation (LLPS)
• RNA processing, ribosome-associated complexes, and RNA regulation in health and disease