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    1. Naturvetenskap och teknik
    2. Matematik och naturvetenskap
    3. Biologi
    4. Biokemi

    Proteins

    Biochemistry and Biotechnology

    AvGary Walsh

    Häftad, Engelska, 2014

    771 kr

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    Beskrivning

    Proteins Biochemistry and Biotechnology 2e is a definitive source of information for all those interested in protein science, and particularly the commercial production and isolation of specific proteins, and their subsequent utilization for applied purposes in industry and medicine. Fully updated throughout with  new or fundamentally revised sections  on proteomics as, bioinformatics, protein glycosylation and  engineering,  well as sections detailing advances in upstream processing and  newer protein  applications such as enzyme-based biofuel production  this new edition has an increased focus on biochemistry to ensure the balance between biochemisty and biotechnology, enhanced with numerous case studies. This second edition is an invaluable text for undergraduates of biochemistry and biotechnology but will also be relevant to students of microbiology, molecular biology, bioinformatics and any branch of the biomedical sciences who require a broad overview of the various medical, diagnostic and industrial uses of proteins. • Provides a comprehensive overview of all aspects of protein biochemisty and protein biotechnology• Includes numerous case studies • Increased focus on protein biochemistry to ensure balance between biochemisty and biotechnology• Includes new section focusing on proteomics as well as sections detailing protein function and enzyme-based biofuel production "With the potential of a standard reference source on the topic, any molecular biotechnologist will profit greatly from having this excellent book. " (Engineering in Life Sciences, 2004; Vol 5; No. 5) “Few texts would be considered competitors, and none compare favorably." (Biochemistry and Molecular Education, July/August 2002) "...The book is well written, making it informative and easy to read..." (The Biochemist, June 2002)

    Produktinformation

    • Utgivningsdatum:2014-05-13
    • Mått:188 x 244 x 33 mm
    • Vikt:771 g
    • Format:Häftad
    • Språk:Engelska
    • Antal sidor:448
    • Upplaga:2
    • Förlag:John Wiley and Sons Ltd
    • ISBN:9780470669853

    Utforska kategorier

    • Biokemi inom Naturvetenskap och teknik

    Mer om författaren

    Gary Walsh, University of Limerick, Ireland.

    Innehållsförteckning

    • Preface xiAbout the Companion Website xiiiChapter 1 Proteins and proteomics 11.1 Proteins, an introduction 11.2 Genes, genomics and proteomics 21.3 Bioinformatics 121.4 Proteomics: goals and applications 14Further reading 22Chapter 2 Protein structure and engineering 252.1 Primary structure 252.2 Higher-level structure 362.3 Protein classification on the basis of structure 412.4 Protein structural stability 452.5 Higher-order structure prediction 472.6 Protein folding 482.7 Intrinsically disordered proteins 502.8 Protein engineering 512.9 Protein post-translational modification 54Further reading 62Chapter 3 Protein sources 653.1 Recombinant versus non-recombinant production 653.2 Approaches to recombinant protein production 673.3 Heterologous protein production in E. coli 723.4 Heterologous production in bacteria other than E. coli 773.5 Heterologous protein production in yeast 773.6 Heterologous protein production in fungi 783.7 Proteins from plants 803.8 Animal tissue as a protein source 843.9 Heterologous protein production in transgenic animals 853.10 Heterologous protein production using animal cell culture 863.11 Insect cell culture systems 87Further reading 88Chapter 4 Protein purification and characterization 914.1 Protein detection and quantification 934.2 Initial recovery of protein 954.3 Removal of whole cells and cell debris 984.4 Concentration 1034.5 Chromatographic purification 1074.6 Protein inactivation and stabilization 1284.7 Protein characterization 137Further reading 139Chapter 5 Large-scale protein production 1415.1 Upstream processing 1415.2 Downstream processing 1545.3 Therapeutic protein production: some special issues 1635.4 Range and medical significance of impurities potentially present in protein-based therapeutic products 166Further reading 175Chapter 6 Therapeutic proteins: blood products, vaccines and enzymes 1776.1 Blood products 1776.2 Anticoagulants 1846.3 Thrombolytic agents 1866.4 Additional blood-related products 1896.5 Vaccine technology 1906.6 Therapeutic enzymes 194Further reading 202Chapter 7 Therapeutic antibodies 2057.1 Antibodies 2057.2 IgG structure and activity 2057.3 Antibody therapeutics: polyclonal antibody preparations 2097.4 Antibody therapeutics: monoclonal antibodies 2117.5 Therapeutic applications of monoclonal antibodies 2207.6 Antibody conjugates 2237.7 Bispecific antibodies 2247.8 Antibody fragments 2257.9 Engineering the antibody glycocomponent 2287.10 Fc fusion proteins 229Further reading 230Chapter 8 Hormones and growth factors used therapeutically 2338.1 Insulin 2338.2 Glucagon 2408.3 Gonadotrophins 2408.4 Growth hormone 2438.5 Erythropoietin 2468.6 Other hormones 2478.7 Growth factors 249Further reading 253Chapter 9 Interferons, interleukins and tumour necrosis factors 2579.1 Regulatory factors: cytokines versus hormones 2579.2 Interferons 2589.3 Interleukins 2649.4 Tumour necrosis factors 271Further reading 274Chapter 10 Proteins used for analytical purposes 27710.1 The IVD sector 27910.2 The basis of analyte detection and quantification 28010.3 Enzymes as diagnostic/analytical reagents 28110.4 Biosensors 28910.5 Antibodies as analytical reagents 295Further reading 309Chapter 11 Industrial enzymes: an introduction 31111.1 Sales value and manufacturers 31311.2 Sources and engineering 31411.3 Environmental benefits 31511.4 Enzyme detection and quantification 31511.5 Immobilized enzymes 31611.6 Extremophiles 31911.7 Enzymes in organic solvents 32411.8 Industrial enzymes: the future 325Further reading 325Chapter 12 Industrial enzymes: proteases and carbohydrases 32712.1 Proteolytic enzymes 32712.2 Carbohydrases 340Further reading 367Chapter 13 Additional industrial enzymes 37113.1 Lipases 37113.2 Penicillin acylase 37513.3 Amino acylase and amino acid production 37813.4 Cyclodextrins and cyclodextrin glycosyltransferase 38013.5 Enzymes and animal nutrition 38213.6 Enzymes in molecular biology 387Further reading 390Chapter 14 Non-catalytic industrial proteins 39314.1 Functional properties of proteins 39314.2 Milk and milk proteins 39714.3 Animal-derived proteins 40814.4 Plant-derived proteins 41114.5 Sweet and taste-modifying proteins 412Further reading 414Index 417
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