Dr Mike Leach - Department of Haematology, Gartnavel General Hospital, Glasgow, UKDr Mark Drummond - Haematology Department, Beatson West of Scotland Cancer Centre, Glasgow, UKDr Allyson Doig - Department of Haematology, Gartnavel Hospital, Glasgow, UK

“I would particularly recommend this book to haematology trainees, as an introduction to, but not as a replacement for, a period spent in an immunophenotyping laboratory.”  (British Journal of Haematology, 14 November 2014)“There is no doubt that this book will find its way firmly onto the bookshelf in every laboratory, and into the work bags of many trainees.”  (Haem Trainee, 1 January 2014)“This is a concise and very practical guide to the use of flow cytometry in hematological and hematopathological diagnosis. While not as detailed in immunophenotyping intricacies as other books of its kind, it shines with its practical approach and expert guidance applied to current clinical practice.”  (Doody’s, 19 July 2013)

• Preface, viiAcknowledgements, viii1 Introduction, 1References, 22 Principles of Flow Cytometry, 3Introduction, 3Sample preparation, 3The flow cytometer, 4The fluidic system, 4The optical system, 5The electronic system, 9Data display, 9Acknowledgements, 18References, 193 Limitations, 20Introduction, 20Clinical context issues, 20Sampling issues, 20Technical issues, 25Interpretation issues, 28Conclusion, 29References, 294 Normal Blood and Bone Marrow Populations, 31Normal stem and precursor cell populations, 31References, 415 Acute Leukaemia, 43Introduction, 43Identification of neoplastic precursor cells, 45Lineage assignment of acute leukaemia, 52Acute myeloid leukaemia, 54Precursor lymphoid neoplasms (acute lymphoblastic leukaemia/lymphoma), 77Examination of cerebro-spinal fluid and serous effusions, 88Identification of genetic abnormalities by flow, 92Conclusion, 93References, 936 Chronic Lymphoid Leukaemias and Exfoliating Lymphoma, 100Normal peripheral blood lymphoid populations, 100Identification of clonal lymphoid populations, 101Identification of clonal B-cell disorders, 101CD5 positive B-cell lymphoproliferative disorders, 102CD10 positive B-cell lymphoproliferative disorders, 116B-cell disorders identified using an extended B-cell panel, 121Identification of clonal T-cell disorders, 125CD4 positive T-cell disorders, 128CD8 positive T-cell disorders, 136CD4/CD8 positive T-cell disorders, 141CD4/8 negative disorders, 141Flow cytometric assessment of serous effusions, 146Flow cytometric assessment of cerebrospinal fluid, 146Summary, 149References, 1497 Myelodysplastic Syndromes and Myeloproliferative Neoplasms, 152Introduction, 152Myelodysplastic syndromes, 152Myelodysplastic/myeloproliferative neoplasms, 163Chronic myeloid leukaemia, 165The BCR-ABL negative myeloproliferative neoplasms, 166Mastocytosis, 167Summary, 170References, 1708 Disorders of Plasma Cells, 173Plasma cell disorders, 173Current diagnostic criteria, 173Plasma cell morphology, 175The applications of immunophenotyping to plasma cell disorders, 178References, 1839 Minimal Residual Disease, 184Introduction, 184Minimal residual disease analysis in the management of acute lymphoblastic leukaemia, 187Conclusions, 199Acknowledgements, 200References, 20010 Red Cells, Leucocytes and Platelets, 202Paroxysmal nocturnal haemoglobinuria, 202Red cell membrane disorders, 208Foetal maternal haemorrhage, 211Lymphocyte subset analysisand immunodeficiency, 214Haemopoietic stem cell enumeration, 214Granulocyte disorders, 215Platelet disorders, 219References, 22111 Reactive and Non-neoplastic Phenomena, 225Peripheral blood, 225Bone marrow, 231Pleural and pericardial fluids, 236Bronchoalveolar lavage specimens, 238Cerebrospinal fluid, 238References, 240Index, 243

Dr Mike Leach - Department of Haematology, Gartnavel General Hospital, Glasgow, UKDr Mark Drummond - Haematology Department, Beatson West of Scotland Cancer Centre, Glasgow, UKDr Allyson Doig - Department of Haematology, Gartnavel Hospital, Glasgow, UK

“I would particularly recommend this book to haematology trainees, as an introduction to, but not as a replacement for, a period spent in an immunophenotyping laboratory.”  (British Journal of Haematology, 14 November 2014)“There is no doubt that this book will find its way firmly onto the bookshelf in every laboratory, and into the work bags of many trainees.”  (Haem Trainee, 1 January 2014)“This is a concise and very practical guide to the use of flow cytometry in hematological and hematopathological diagnosis. While not as detailed in immunophenotyping intricacies as other books of its kind, it shines with its practical approach and expert guidance applied to current clinical practice.”  (Doody’s, 19 July 2013)

• Preface, viiAcknowledgements, viii1 Introduction, 1References, 22 Principles of Flow Cytometry, 3Introduction, 3Sample preparation, 3The flow cytometer, 4The fluidic system, 4The optical system, 5The electronic system, 9Data display, 9Acknowledgements, 18References, 193 Limitations, 20Introduction, 20Clinical context issues, 20Sampling issues, 20Technical issues, 25Interpretation issues, 28Conclusion, 29References, 294 Normal Blood and Bone Marrow Populations, 31Normal stem and precursor cell populations, 31References, 415 Acute Leukaemia, 43Introduction, 43Identification of neoplastic precursor cells, 45Lineage assignment of acute leukaemia, 52Acute myeloid leukaemia, 54Precursor lymphoid neoplasms (acute lymphoblastic leukaemia/lymphoma), 77Examination of cerebro-spinal fluid and serous effusions, 88Identification of genetic abnormalities by flow, 92Conclusion, 93References, 936 Chronic Lymphoid Leukaemias and Exfoliating Lymphoma, 100Normal peripheral blood lymphoid populations, 100Identification of clonal lymphoid populations, 101Identification of clonal B-cell disorders, 101CD5 positive B-cell lymphoproliferative disorders, 102CD10 positive B-cell lymphoproliferative disorders, 116B-cell disorders identified using an extended B-cell panel, 121Identification of clonal T-cell disorders, 125CD4 positive T-cell disorders, 128CD8 positive T-cell disorders, 136CD4/CD8 positive T-cell disorders, 141CD4/8 negative disorders, 141Flow cytometric assessment of serous effusions, 146Flow cytometric assessment of cerebrospinal fluid, 146Summary, 149References, 1497 Myelodysplastic Syndromes and Myeloproliferative Neoplasms, 152Introduction, 152Myelodysplastic syndromes, 152Myelodysplastic/myeloproliferative neoplasms, 163Chronic myeloid leukaemia, 165The BCR-ABL negative myeloproliferative neoplasms, 166Mastocytosis, 167Summary, 170References, 1708 Disorders of Plasma Cells, 173Plasma cell disorders, 173Current diagnostic criteria, 173Plasma cell morphology, 175The applications of immunophenotyping to plasma cell disorders, 178References, 1839 Minimal Residual Disease, 184Introduction, 184Minimal residual disease analysis in the management of acute lymphoblastic leukaemia, 187Conclusions, 199Acknowledgements, 200References, 20010 Red Cells, Leucocytes and Platelets, 202Paroxysmal nocturnal haemoglobinuria, 202Red cell membrane disorders, 208Foetal maternal haemorrhage, 211Lymphocyte subset analysisand immunodeficiency, 214Haemopoietic stem cell enumeration, 214Granulocyte disorders, 215Platelet disorders, 219References, 22111 Reactive and Non-neoplastic Phenomena, 225Peripheral blood, 225Bone marrow, 231Pleural and pericardial fluids, 236Bronchoalveolar lavage specimens, 238Cerebrospinal fluid, 238References, 240Index, 243

Dr Mike Leach - Department of Haematology, Gartnavel General Hospital, Glasgow, UKDr Mark Drummond - Haematology Department, Beatson West of Scotland Cancer Centre, Glasgow, UKDr Allyson Doig - Department of Haematology, Gartnavel Hospital, Glasgow, UK

“I would particularly recommend this book to haematology trainees, as an introduction to, but not as a replacement for, a period spent in an immunophenotyping laboratory.”  (British Journal of Haematology, 14 November 2014)“There is no doubt that this book will find its way firmly onto the bookshelf in every laboratory, and into the work bags of many trainees.”  (Haem Trainee, 1 January 2014)“This is a concise and very practical guide to the use of flow cytometry in hematological and hematopathological diagnosis. While not as detailed in immunophenotyping intricacies as other books of its kind, it shines with its practical approach and expert guidance applied to current clinical practice.”  (Doody’s, 19 July 2013)

• Preface, viiAcknowledgements, viii1 Introduction, 1References, 22 Principles of Flow Cytometry, 3Introduction, 3Sample preparation, 3The flow cytometer, 4The fluidic system, 4The optical system, 5The electronic system, 9Data display, 9Acknowledgements, 18References, 193 Limitations, 20Introduction, 20Clinical context issues, 20Sampling issues, 20Technical issues, 25Interpretation issues, 28Conclusion, 29References, 294 Normal Blood and Bone Marrow Populations, 31Normal stem and precursor cell populations, 31References, 415 Acute Leukaemia, 43Introduction, 43Identification of neoplastic precursor cells, 45Lineage assignment of acute leukaemia, 52Acute myeloid leukaemia, 54Precursor lymphoid neoplasms (acute lymphoblastic leukaemia/lymphoma), 77Examination of cerebro-spinal fluid and serous effusions, 88Identification of genetic abnormalities by flow, 92Conclusion, 93References, 936 Chronic Lymphoid Leukaemias and Exfoliating Lymphoma, 100Normal peripheral blood lymphoid populations, 100Identification of clonal lymphoid populations, 101Identification of clonal B-cell disorders, 101CD5 positive B-cell lymphoproliferative disorders, 102CD10 positive B-cell lymphoproliferative disorders, 116B-cell disorders identified using an extended B-cell panel, 121Identification of clonal T-cell disorders, 125CD4 positive T-cell disorders, 128CD8 positive T-cell disorders, 136CD4/CD8 positive T-cell disorders, 141CD4/8 negative disorders, 141Flow cytometric assessment of serous effusions, 146Flow cytometric assessment of cerebrospinal fluid, 146Summary, 149References, 1497 Myelodysplastic Syndromes and Myeloproliferative Neoplasms, 152Introduction, 152Myelodysplastic syndromes, 152Myelodysplastic/myeloproliferative neoplasms, 163Chronic myeloid leukaemia, 165The BCR-ABL negative myeloproliferative neoplasms, 166Mastocytosis, 167Summary, 170References, 1708 Disorders of Plasma Cells, 173Plasma cell disorders, 173Current diagnostic criteria, 173Plasma cell morphology, 175The applications of immunophenotyping to plasma cell disorders, 178References, 1839 Minimal Residual Disease, 184Introduction, 184Minimal residual disease analysis in the management of acute lymphoblastic leukaemia, 187Conclusions, 199Acknowledgements, 200References, 20010 Red Cells, Leucocytes and Platelets, 202Paroxysmal nocturnal haemoglobinuria, 202Red cell membrane disorders, 208Foetal maternal haemorrhage, 211Lymphocyte subset analysisand immunodeficiency, 214Haemopoietic stem cell enumeration, 214Granulocyte disorders, 215Platelet disorders, 219References, 22111 Reactive and Non-neoplastic Phenomena, 225Peripheral blood, 225Bone marrow, 231Pleural and pericardial fluids, 236Bronchoalveolar lavage specimens, 238Cerebrospinal fluid, 238References, 240Index, 243