Confocal and Two-Photon Microscopy

Alberto Diaspro was born in Genoa, Italy, on April 7, 1959, and went on to receive his doctoral degree in Electronic Engineering from the University of Genoa in 1983. He is an investigator and professor for the Department of Physics at the University of Genoa, and a member of the National Institute for the Physics of Matter (INFM). Dr. Diaspro also holds positions on the editorial boards of several international journals, namely: Microscopy Research and Technique, Journal of Computer Assisted Microscopy, Journal of Biomedical Optics and European Journal of Histochemistry, and he is the author of more than 100 international scientific publications. His main research activities aim at the study of biostructures both in situ and in vitro, but his many interests include the design, realization and utilization of biophysical instrumentation as conventional and confocal microscopy, two-photon fluorescence microscopy and spectroscopy architecture, differential scanning calorimetry, scanning probe microscopy (STM, SNOM, AFM), polarized light scattering, and signal and image digital processing.

"This is an excellent book that is a 'must-have' for any laboratory that is interested in novel forms of microscopy." (Biomolecular Engineering, November 2004) "…well presented with handsome images and figures…a useful source of reference…" (Annals of Biomedical Engineering, July 2002)

• Foreword (G. Brakenhoff). Preface. Acknowledgements. Contributors. The Generalized Microscope (C. Sheppard). Confocal Microscopy: Basic Principles and Architectures (T. Wilson). Two-Photon Microscopy: Basic Principles and Architectures (A. Diaspro & C. Sheppard). Cross-Sections of Fluorescent Molecules in Multiphoton Microscopy (C. Xu). Resolution and Contrast in Confocal and Two-Photon Microscopy (J. Jonkman and E. Stelzer). The Role of Pinhole Size in High-Aperture Two-and Three-Photon Microscopy (P. Torok & C. Sheppard). Aberrations and Penetration in In-Depth Confocal and Two-Photon-Excitation Microscopy (C. de Grauw, et al.). Group Velocity Dispersion and Fiber Delivery in Multiphoton Laser Scanning Microscopy (R. Wolleschensky, et al.). Cellular and Subcellular Perturbations During Multiphoton Microscopy (K. Konig & U. Tirlapur). Pracitcal Multiphoton Microscopy (J. Girkin & D. Wokosin). Sampling, Resolution and Digital Image Processing in the Spatial and Fourier Domains (K. Castleman). Image-Restoration Methods: Basics and Algorithms (P. Boccacci & M. Bertero). Two-Photon Excitation Fluorescence Microscopy Imaging in Xenopus and Transgenic Mouse Embryos (A. Periasamy, et al.). Multiple Color Fluorescence Imaging in Microscopy (K. Castleman). Stereological Methods for Estimating Geometrical Parameters of Microscopic Structure by Three-Dimensional Imaging (L. Kubinova, et al.). Imaging Live Cells in 3D Using Wide-Field Microscopy with Image Restoration (W. Carrington). Confocal Microscopy in the Study of the Cell Nucleus (L. Neri, et al.). Confocal Imaging of Neuronal Growth and Morphology in Brain Slices (I. Skaliora & S. Pagakis). FISH Imaging (M. Kozubek). Two-Photon Imaging of Tissue Physiology based on Endogenous Fluorophores (L. Hsu, et al.). Two-Photon Near-Infrared Femtosecond Laser Scanning Microscopy in Plant Biology (U. Tirlapur & K. Konig). Two-Photon Excitation Microscopy for Image Spectroscopy and Biochemistry of Tissues, Cells, Organelles, and Lipid Vesicles Under Physiological Conditions (T. Parasassi, et al.). Real-Time In Situ Calcium Imaging with Single- and Two-Photon Confocal Microscopy (K. Fujita & T. Takamatsu). Superresolution in Fluorescence Confocal Microscopy and in DVD Optical Storage (R. Pike). Photobleaching by Confocal Microscopy (J. McNally & C. Smith). Two-Photon Laser Scanning Microscopy for Characterization of Integrated Circuits and Optoelectronics (C. Xu). Index.