GORDANA VUNJAK-NOVAKOVIC, PhD, is Professor in the Department of Biomedical Engineering at Columbia University, New York. She is the author of more than 125 papers and twenty-five book chapters on biotechnology, biomechanics, orthopedics, and tissue engineering. R. IAN FRESHNEY, PhD, is Senior Research Fellow in the Centre for Oncology and Applied Pharmacolgy at the University of Glasgow. He is the author or editor of numerous books and a world-renowned expert on cell culture technique.

"…among the best works on this subject. Recommended for all science and medical libraries." (E-STREAMS, September 2007) "The editors have brought together an outstanding group of experts to describe cell culture methods and applications for tissue engineering." (Doody's Health Services)

• Preface viiList of Abbreviations xiPart I: Cell Culture1. Basic Principles of Cell Culture 3R. Ian Freshney2. Mesenchymal Stem Cells for Tissue Engineering 23Donald P. Lennon and Arnold I. Caplan3. Human Embryonic Stem Cell Culture for Tissue Engineering 61Shulamit Levenberg, Ali Khademhosseini, Mara Macdonald, Jason Fuller, and Robert Langer4. Cell Sources for Cartilage Tissue Engineering 83Brian Johnstone, Jung Yoo, and Matthew Stewart5. Lipid-Mediated Gene Transfer for Cartilage Tissue Engineering 113Henning MadryPart II: Tissue Engineering6. Tissue Engineering: Basic Considerations 131Gordana Vunjak-Novakovic7. Tissue Engineering of Articular Cartilage 157Koichi Masuda and Robert L. Sah8. Ligament Tissue Engineering 191Jingsong Chen, Jodie Moreau, Rebecca Horan, Adam Collette, Diah Bramano, Vladimir Volloch, John Richmond, Gordana Vunjak-Novakovic, David L. Kaplan, and Gregory H. Altman9. Cellular Photoencapsulation in Hydrogels 213Jennifer Elisseeff, Melanie Ruffner, Tae-Gyun Kim, and Christopher Williams10. Tissue Engineering Human Skeletal Muscle for Clinical Applications 239Janet Shansky, Paulette Ferland, Sharon McGuire, Courtney Powell, Michael DelTatto, Martin Nackman, James Hennessey, and Herman H. Vandenburgh11. Engineered Heart Tissue 259Thomas Eschenhagen and Wolfgang H. Zimmermann12. Tissue-Engineered Blood Vessels 293Rebecca Y. Klinger and Laura E. Niklason13. Tissue Engineering of Bone 323Sandra Hofmann, David Kaplan, Gordana Vunjak-Novakovic, and Lorenz Meinel14. Culture of Neuroendocrine and Neuronal Cells for Tissue Engineering 375Peter I. Lelkes, Brian R. Unsworth, Samuel Saporta, Don F. Cameron, and Gianluca Gallo15. Tissue Engineering of the Liver 417Gregory H. Underhill, Jennifer Felix, Jared W. Allen, Valerie Liu Tsang, Salman R. Khetani, and Sangeeta N. BhatiaSuppliers List 473Glossary 483Index 491

GORDANA VUNJAK-NOVAKOVIC, PhD, is Professor in the Department of Biomedical Engineering at Columbia University, New York. She is the author of more than 125 papers and twenty-five book chapters on biotechnology, biomechanics, orthopedics, and tissue engineering. R. IAN FRESHNEY, PhD, is Senior Research Fellow in the Centre for Oncology and Applied Pharmacolgy at the University of Glasgow. He is the author or editor of numerous books and a world-renowned expert on cell culture technique.

"…among the best works on this subject. Recommended for all science and medical libraries." (E-STREAMS, September 2007) "The editors have brought together an outstanding group of experts to describe cell culture methods and applications for tissue engineering." (Doody's Health Services)

• Preface viiList of Abbreviations xiPart I: Cell Culture1. Basic Principles of Cell Culture 3R. Ian Freshney2. Mesenchymal Stem Cells for Tissue Engineering 23Donald P. Lennon and Arnold I. Caplan3. Human Embryonic Stem Cell Culture for Tissue Engineering 61Shulamit Levenberg, Ali Khademhosseini, Mara Macdonald, Jason Fuller, and Robert Langer4. Cell Sources for Cartilage Tissue Engineering 83Brian Johnstone, Jung Yoo, and Matthew Stewart5. Lipid-Mediated Gene Transfer for Cartilage Tissue Engineering 113Henning MadryPart II: Tissue Engineering6. Tissue Engineering: Basic Considerations 131Gordana Vunjak-Novakovic7. Tissue Engineering of Articular Cartilage 157Koichi Masuda and Robert L. Sah8. Ligament Tissue Engineering 191Jingsong Chen, Jodie Moreau, Rebecca Horan, Adam Collette, Diah Bramano, Vladimir Volloch, John Richmond, Gordana Vunjak-Novakovic, David L. Kaplan, and Gregory H. Altman9. Cellular Photoencapsulation in Hydrogels 213Jennifer Elisseeff, Melanie Ruffner, Tae-Gyun Kim, and Christopher Williams10. Tissue Engineering Human Skeletal Muscle for Clinical Applications 239Janet Shansky, Paulette Ferland, Sharon McGuire, Courtney Powell, Michael DelTatto, Martin Nackman, James Hennessey, and Herman H. Vandenburgh11. Engineered Heart Tissue 259Thomas Eschenhagen and Wolfgang H. Zimmermann12. Tissue-Engineered Blood Vessels 293Rebecca Y. Klinger and Laura E. Niklason13. Tissue Engineering of Bone 323Sandra Hofmann, David Kaplan, Gordana Vunjak-Novakovic, and Lorenz Meinel14. Culture of Neuroendocrine and Neuronal Cells for Tissue Engineering 375Peter I. Lelkes, Brian R. Unsworth, Samuel Saporta, Don F. Cameron, and Gianluca Gallo15. Tissue Engineering of the Liver 417Gregory H. Underhill, Jennifer Felix, Jared W. Allen, Valerie Liu Tsang, Salman R. Khetani, and Sangeeta N. BhatiaSuppliers List 473Glossary 483Index 491

GORDANA VUNJAK-NOVAKOVIC, PhD, is Professor in the Department of Biomedical Engineering at Columbia University, New York. She is the author of more than 125 papers and twenty-five book chapters on biotechnology, biomechanics, orthopedics, and tissue engineering. R. IAN FRESHNEY, PhD, is Senior Research Fellow in the Centre for Oncology and Applied Pharmacolgy at the University of Glasgow. He is the author or editor of numerous books and a world-renowned expert on cell culture technique.

"…among the best works on this subject. Recommended for all science and medical libraries." (E-STREAMS, September 2007) "The editors have brought together an outstanding group of experts to describe cell culture methods and applications for tissue engineering." (Doody's Health Services)

• Preface viiList of Abbreviations xiPart I: Cell Culture1. Basic Principles of Cell Culture 3R. Ian Freshney2. Mesenchymal Stem Cells for Tissue Engineering 23Donald P. Lennon and Arnold I. Caplan3. Human Embryonic Stem Cell Culture for Tissue Engineering 61Shulamit Levenberg, Ali Khademhosseini, Mara Macdonald, Jason Fuller, and Robert Langer4. Cell Sources for Cartilage Tissue Engineering 83Brian Johnstone, Jung Yoo, and Matthew Stewart5. Lipid-Mediated Gene Transfer for Cartilage Tissue Engineering 113Henning MadryPart II: Tissue Engineering6. Tissue Engineering: Basic Considerations 131Gordana Vunjak-Novakovic7. Tissue Engineering of Articular Cartilage 157Koichi Masuda and Robert L. Sah8. Ligament Tissue Engineering 191Jingsong Chen, Jodie Moreau, Rebecca Horan, Adam Collette, Diah Bramano, Vladimir Volloch, John Richmond, Gordana Vunjak-Novakovic, David L. Kaplan, and Gregory H. Altman9. Cellular Photoencapsulation in Hydrogels 213Jennifer Elisseeff, Melanie Ruffner, Tae-Gyun Kim, and Christopher Williams10. Tissue Engineering Human Skeletal Muscle for Clinical Applications 239Janet Shansky, Paulette Ferland, Sharon McGuire, Courtney Powell, Michael DelTatto, Martin Nackman, James Hennessey, and Herman H. Vandenburgh11. Engineered Heart Tissue 259Thomas Eschenhagen and Wolfgang H. Zimmermann12. Tissue-Engineered Blood Vessels 293Rebecca Y. Klinger and Laura E. Niklason13. Tissue Engineering of Bone 323Sandra Hofmann, David Kaplan, Gordana Vunjak-Novakovic, and Lorenz Meinel14. Culture of Neuroendocrine and Neuronal Cells for Tissue Engineering 375Peter I. Lelkes, Brian R. Unsworth, Samuel Saporta, Don F. Cameron, and Gianluca Gallo15. Tissue Engineering of the Liver 417Gregory H. Underhill, Jennifer Felix, Jared W. Allen, Valerie Liu Tsang, Salman R. Khetani, and Sangeeta N. BhatiaSuppliers List 473Glossary 483Index 491