Introduction to Proteomics

NAWIN C. MISHRA, PhD, is an Emeritus Professor of Molecular Genetics and Genomics at the Department of Biological Sciences at the University of South Carolina. Professor Mishra has also served as a scientific consultant to the FAO of the United Nations, as Chairman of the Program Committee of the Genetics Society of America, and on the review panel of the Human Genome Project for the United States DOE. Professor Mishra has published over sixty articles in prominent research journals, including Nature, the Proceedings of the United States National Academy of Sciences, and Genetics.

"The Author offers an expert perspective on the entire field, including: Easily accessible overview of the principles of proteomics; Coverage of real-world, cutting-edge medical applications, including personalized medicine; Clear guidelines on how to operate the complex instrumentation involved in proteomics; Discussion of the future of proteomics". (Anticancer Research, 1 August 2010) This book provides a highly authoritative introduction to the promising and fast-advancing field of proteomics, examining the role proteomics plays in the study of biological systems in general and disease in particular. It helps readers understand the structure, function, and interactions of proteins and how this knowledge is used for identifying diseases and developing new drugs." (Forbes.com, 1 November 2010)"For undergraduate and graduate biology and non-biology students and new researchers, Mishra (molecular genetics and genomics, U. of South Carolina) introduces the field of proteomics and its role in the study of biological systems and disease." (Book News, September 2010)

• FOREWORD. PREFACE.ABOUT THE AUTHOR.CHAPTER 1 HISTORICAL PERSPECTIVES.1.1. Introduction to Proteomics.1.2. Proteome and Proteomics.1.3. Genetics of Proteins.1.4. Molecular Biology of Genes and Proteins.1.5. Protein Chemistry Before Proteomics.References.Further Reading.CHAPTER 2 PROTEOMICS—RELATION TO GENOMICS, BIOINFORMATICS.2.1. Genomics.2.2. Bioinformatics and Computational Biology.References.Further Reading.CHAPTER 3 METHODOLOGY FOR SEPARATION AND IDENTIFICATION OF PROTEINS AND THEIR INTERACTIONS.3.1. Separation of Proteins Via a Multidimensional Approach.3.2. Determination of the Primary Structure of Proteins.3.3. Determination of the 3D Structure of a Protein.3.4. Determination of the Amount of Proteins.3.5. Structural and Functional Proteomics.References.Further Reading.CHAPTER 4 PROTEOMICS OF PROTEIN MODIFICATIONS.4.1. Phosphorylation and Phosphoproteomics.4.2. Glycosylation and Glycoproteomics.4.3. Ubiquitination and Ubiquitinomics.4.4. Miscellaneous Modifications of Proteins.References.Further Reading.CHAPTER 5 PROTEOMICS OF PROTEIN–PROTEIN INTERACTIONS/INTERACTOMES.5.1. Protein—Protein Interactions (PPI) in Vivo.5.2. Analysis of Protein Interactions in Vitro.5.3. Analysis of Protein Interactions in Silico.5.4. Synthetic Genetic Methods to Determine Protein Interactions.5.5. Interactomes.5.6. Evolution and Conservation of Interactomes.5.7. Interactomes and the Complexity of Organisms: It is the Number of Interactomes that Matters in Understanding the Complexity of an Organism and not the Number of Genes.5.8. Interaction of Proteins with Small Molecules.References.Further Reading.CHAPTER 6 APPLICATIONS OF PROTEOMICS I: PROTEOMICS, HUMAN DISEASE, AND MEDICINE.6.1. Diseasome.6.2. Medical Proteomics.6.3. Clinical Proteomics.6.4. Metaproteomics and Human Health.6.5. Proteomics in Biotechnology and Industry of Drug Production.6.6. Metaproteomics of Microbial Fermentation.6.7. Beef Industry.6.8. Bioterrorism and Biodefense.References.Further Reading.CHAPTER 7 PROTEOMICS—FUTURE DEVELOPMENTS.7.1. Technical Scope of Proteomics—Beyond Protein Identification.7.2. Scientific Scope of Proteomics—Control of Epigenesis.7.3. Medical Scope of Proteomics.7.4. Proteomics, Energy Production, and Bioremediation.7.5. Proteomics and Biodefense.References.Further Reading.INDEX.