C. Neal Stewart is Racheff Chair of Excellence in Plant Molecular Genetics and Professor, Department of Plant Sciences, University of Tennessee. Alisher Touraev is Group Leader and Associated Professor of the Institute of Microbiology and Genetics at Vienna University. Vitaly Citovsky is a Professor in the Department of Biochemistry and Cell Biology at the State University of New York, Stony Brook. Tzvi Tzfira is Assistant Professor in Department of Molecular, Cellular, and Developmental Biology at the University of Michigan

“Books such as this are invaluable for any laboratory that performs recombinant technology in plants and come not only highly recommended, but are essential reading for all molecular biology students.”  (The Quarterly Review of Biology, 1 September 2012)

• Contributors. Preface.SECTION 1 Agrobacterium-Mediated Transformation.Chapter 1 Host Factors Involved in Genetic Transformation of Plant Cells byAgrobacterium (BENOˆIT LACROIX, ADI ZALTSMAN, AND VITALY CITOVSKY).Chapter 2 Genomics of Agrobacterium–Plant Interaction: An Approach to Refine the Plant Transformation Technology (AJITH ANAND, ZARIR E. VAGHCHHIPAWALA, AND KIRANKUMAR S. MYSORE).SECTION 2 Other Transformation Technologies.Chapter 3 Particle Bombardment: An Established Weapon in the Arsenal of Plant Biotechnologists (THEODORE M. KLEIN).Chapter 4 A Novel Gene Delivery System in Plants with Calcium Alginate Micro-Beads (NAOKI WADA, SHIN’ICHIRO KAJIYAMA, NARUEMON KHEMKLADNGOEN, AND KIICHI FUKUI).Chapter 5 Pollen Transformation Technologies (TATIANA RESCH AND ALISHER TOURAEV).Chapter 6 Intragenic Vectors and Marker-Free Transformation: Tools for a Greener Biotechnology (CAIUS M. ROMMENS, ANTHONY CONNER, HUA YAN, AND ZAC HANLEY).Chapter 7 Visualizing Transgene Expression (JOHN J. FINER).SECTION 3 Vectors, Promoters, and Other Tools for Plant Transformation.Chapter 8 Current State and Perspective of Binary Vectors and Superbinary Vectors (TOSHIYUKI KOMORI AND TOSHIHIKO KOMARI).Chapter 9 Novel Dual Binary Vectors (pCLEAN) for Plant Transformation (PHILIPPE VAIN).Chapter 10 pORE Modular Vectors for Plant Transformation (DWAYNE D. HEGEDUS).Chapter 11 pANIC: A Versatile Set of Gateway-Compatible Vectors for Gene Overexpression and RNAi-Mediated Down-Regulation in Monocots (DAVID G.J. MANN, PETER R. LAFAYETTE, LAURA L. ABERCROMBIE, WAYNE A. PARROTT, AND C. NEAL STEWART, JR.).SECTION 4 Transgene Integration, Stability, Methylation, Silencing.Chapter 12 Understanding and Avoiding Transgene Silencing (MICHAEL W. GRAHAM, STEPHEN R. MUDGE, PETER R. STERNES, AND ROBERT G. BIRCH).Chapter 13 Site-Specific Recombination for Precise and “Clean” Transgene Integration in Plant Genome (VIBHA SRIVASTAVA AND DAVID W. OW).SECTION 5 Selection Systems, Marker-Free Transformation.Chapter 14 Selectable Marker Genes: Types and Interactions (BRIAN MIKI, ASHRAF ABDEEN, YUZUKI MANABE, SOUAD EL OUAKFAOUI, DOUGLAS A. JOHNSON, LORETA GUDYNAITE-SAVITCH, MING HU, AND PHIL MACDONALD).Chapter 15 Transformation Methods for Obtaining Marker-Free Genetically Modified Plants (JAN G. SCHAART, FRANS A. KRENS, ANNE-MARIE A. WOLTERS, AND RICHARD G.F. VISSER).Chapter 16 Intellectual Property Aspects of Plant Transformation (JIM M. DUNWELL).Index.Color Plate between pages 82 and 83.

C. Neal Stewart is Racheff Chair of Excellence in Plant Molecular Genetics and Professor, Department of Plant Sciences, University of Tennessee. Alisher Touraev is Group Leader and Associated Professor of the Institute of Microbiology and Genetics at Vienna University. Vitaly Citovsky is a Professor in the Department of Biochemistry and Cell Biology at the State University of New York, Stony Brook. Tzvi Tzfira is Assistant Professor in Department of Molecular, Cellular, and Developmental Biology at the University of Michigan

“Books such as this are invaluable for any laboratory that performs recombinant technology in plants and come not only highly recommended, but are essential reading for all molecular biology students.”  (The Quarterly Review of Biology, 1 September 2012)

• Contributors. Preface.SECTION 1 Agrobacterium-Mediated Transformation.Chapter 1 Host Factors Involved in Genetic Transformation of Plant Cells byAgrobacterium (BENOˆIT LACROIX, ADI ZALTSMAN, AND VITALY CITOVSKY).Chapter 2 Genomics of Agrobacterium–Plant Interaction: An Approach to Refine the Plant Transformation Technology (AJITH ANAND, ZARIR E. VAGHCHHIPAWALA, AND KIRANKUMAR S. MYSORE).SECTION 2 Other Transformation Technologies.Chapter 3 Particle Bombardment: An Established Weapon in the Arsenal of Plant Biotechnologists (THEODORE M. KLEIN).Chapter 4 A Novel Gene Delivery System in Plants with Calcium Alginate Micro-Beads (NAOKI WADA, SHIN’ICHIRO KAJIYAMA, NARUEMON KHEMKLADNGOEN, AND KIICHI FUKUI).Chapter 5 Pollen Transformation Technologies (TATIANA RESCH AND ALISHER TOURAEV).Chapter 6 Intragenic Vectors and Marker-Free Transformation: Tools for a Greener Biotechnology (CAIUS M. ROMMENS, ANTHONY CONNER, HUA YAN, AND ZAC HANLEY).Chapter 7 Visualizing Transgene Expression (JOHN J. FINER).SECTION 3 Vectors, Promoters, and Other Tools for Plant Transformation.Chapter 8 Current State and Perspective of Binary Vectors and Superbinary Vectors (TOSHIYUKI KOMORI AND TOSHIHIKO KOMARI).Chapter 9 Novel Dual Binary Vectors (pCLEAN) for Plant Transformation (PHILIPPE VAIN).Chapter 10 pORE Modular Vectors for Plant Transformation (DWAYNE D. HEGEDUS).Chapter 11 pANIC: A Versatile Set of Gateway-Compatible Vectors for Gene Overexpression and RNAi-Mediated Down-Regulation in Monocots (DAVID G.J. MANN, PETER R. LAFAYETTE, LAURA L. ABERCROMBIE, WAYNE A. PARROTT, AND C. NEAL STEWART, JR.).SECTION 4 Transgene Integration, Stability, Methylation, Silencing.Chapter 12 Understanding and Avoiding Transgene Silencing (MICHAEL W. GRAHAM, STEPHEN R. MUDGE, PETER R. STERNES, AND ROBERT G. BIRCH).Chapter 13 Site-Specific Recombination for Precise and “Clean” Transgene Integration in Plant Genome (VIBHA SRIVASTAVA AND DAVID W. OW).SECTION 5 Selection Systems, Marker-Free Transformation.Chapter 14 Selectable Marker Genes: Types and Interactions (BRIAN MIKI, ASHRAF ABDEEN, YUZUKI MANABE, SOUAD EL OUAKFAOUI, DOUGLAS A. JOHNSON, LORETA GUDYNAITE-SAVITCH, MING HU, AND PHIL MACDONALD).Chapter 15 Transformation Methods for Obtaining Marker-Free Genetically Modified Plants (JAN G. SCHAART, FRANS A. KRENS, ANNE-MARIE A. WOLTERS, AND RICHARD G.F. VISSER).Chapter 16 Intellectual Property Aspects of Plant Transformation (JIM M. DUNWELL).Index.Color Plate between pages 82 and 83.

C. Neal Stewart is Racheff Chair of Excellence in Plant Molecular Genetics and Professor, Department of Plant Sciences, University of Tennessee. Alisher Touraev is Group Leader and Associated Professor of the Institute of Microbiology and Genetics at Vienna University. Vitaly Citovsky is a Professor in the Department of Biochemistry and Cell Biology at the State University of New York, Stony Brook. Tzvi Tzfira is Assistant Professor in Department of Molecular, Cellular, and Developmental Biology at the University of Michigan

“Books such as this are invaluable for any laboratory that performs recombinant technology in plants and come not only highly recommended, but are essential reading for all molecular biology students.”  (The Quarterly Review of Biology, 1 September 2012)

• Contributors. Preface.SECTION 1 Agrobacterium-Mediated Transformation.Chapter 1 Host Factors Involved in Genetic Transformation of Plant Cells byAgrobacterium (BENOˆIT LACROIX, ADI ZALTSMAN, AND VITALY CITOVSKY).Chapter 2 Genomics of Agrobacterium–Plant Interaction: An Approach to Refine the Plant Transformation Technology (AJITH ANAND, ZARIR E. VAGHCHHIPAWALA, AND KIRANKUMAR S. MYSORE).SECTION 2 Other Transformation Technologies.Chapter 3 Particle Bombardment: An Established Weapon in the Arsenal of Plant Biotechnologists (THEODORE M. KLEIN).Chapter 4 A Novel Gene Delivery System in Plants with Calcium Alginate Micro-Beads (NAOKI WADA, SHIN’ICHIRO KAJIYAMA, NARUEMON KHEMKLADNGOEN, AND KIICHI FUKUI).Chapter 5 Pollen Transformation Technologies (TATIANA RESCH AND ALISHER TOURAEV).Chapter 6 Intragenic Vectors and Marker-Free Transformation: Tools for a Greener Biotechnology (CAIUS M. ROMMENS, ANTHONY CONNER, HUA YAN, AND ZAC HANLEY).Chapter 7 Visualizing Transgene Expression (JOHN J. FINER).SECTION 3 Vectors, Promoters, and Other Tools for Plant Transformation.Chapter 8 Current State and Perspective of Binary Vectors and Superbinary Vectors (TOSHIYUKI KOMORI AND TOSHIHIKO KOMARI).Chapter 9 Novel Dual Binary Vectors (pCLEAN) for Plant Transformation (PHILIPPE VAIN).Chapter 10 pORE Modular Vectors for Plant Transformation (DWAYNE D. HEGEDUS).Chapter 11 pANIC: A Versatile Set of Gateway-Compatible Vectors for Gene Overexpression and RNAi-Mediated Down-Regulation in Monocots (DAVID G.J. MANN, PETER R. LAFAYETTE, LAURA L. ABERCROMBIE, WAYNE A. PARROTT, AND C. NEAL STEWART, JR.).SECTION 4 Transgene Integration, Stability, Methylation, Silencing.Chapter 12 Understanding and Avoiding Transgene Silencing (MICHAEL W. GRAHAM, STEPHEN R. MUDGE, PETER R. STERNES, AND ROBERT G. BIRCH).Chapter 13 Site-Specific Recombination for Precise and “Clean” Transgene Integration in Plant Genome (VIBHA SRIVASTAVA AND DAVID W. OW).SECTION 5 Selection Systems, Marker-Free Transformation.Chapter 14 Selectable Marker Genes: Types and Interactions (BRIAN MIKI, ASHRAF ABDEEN, YUZUKI MANABE, SOUAD EL OUAKFAOUI, DOUGLAS A. JOHNSON, LORETA GUDYNAITE-SAVITCH, MING HU, AND PHIL MACDONALD).Chapter 15 Transformation Methods for Obtaining Marker-Free Genetically Modified Plants (JAN G. SCHAART, FRANS A. KRENS, ANNE-MARIE A. WOLTERS, AND RICHARD G.F. VISSER).Chapter 16 Intellectual Property Aspects of Plant Transformation (JIM M. DUNWELL).Index.Color Plate between pages 82 and 83.