Protocols in Molecular Parasitology

...well-written and thoughtfully assembled...will be welcomed by recent arrivals...and regularly utilized by instructors of future investigators. - Analytical Biochemistry

• Culturing and Biological Cloning of Trypanosoma brucei.- Culturing and Biological Cloning of Trypanosoma cruzi.- In Vitro Cultivation and Biological Cloning of Leishmania.- Simple In Vitro Cultivation of the Malaria Parasite Plasmodium falciparum (Erythrocytic Stages).- Synchronization and Cloning of Malaria Parasites.- The Culture and Preparation of Gametocytes of Plasmodium falciparum for Immunochemical, Molecular, and Mosquito Infectivity Studies.- The Culture of Schistosoma mansoni and Production of Life Cycle Stages.- Preparation of DNA and RNA from Trypanosoma brucei.- Isolation of RNA and DNA from Trypanosoma cruzi.- Isolation of DNA and RNA from Leishmania.- The Extraction and Purification of DNA and RNA from In Vitro Cultures of the Malaria Parasite Plasmodium falciparum.- Extraction and Purification of Nucleic Acids from Schistosomes.- Application of DNA and Whole Organisms to Filter Supports for DNA Probe Analysis: Dot, Slot, and Touch Blotting.- The Development and Use of Repetitive Sequences as DNA Probes for Parasite Detection and Species Identification.- Analysis of the DNA of Parasitic Protozoa by Flow Cytometry.- PCR orLowLevel Detection of Malaria Parasites in Blood.- PCR Methods for Identification of Point Mutations and Gene Rearrangements.- Antigenic Typing of Field Isolates of Plasmodium by DNA Techniques.- Ribosomal RNA Probes for Detection and Identification of Species.- Rapid Sequencing of Parasite rRNA.- Antibody Screening of Expression Libraries.- Antibody Select Procedure for Characterization of Expression Clones.- Isolation of Parasite Genes Using Synthetic Oligonucleotides.- Chromosome Mapping Methods for Parasitic Protozoa.- Transfection ofLeishmania and Trypanosoma brucei by Electroporation.- Two-Dimensional Polyacrylamide Gel Electrophoresis.- Isoenzyme Electrophoresis for Parasite Characterization.- The Surface Labeling of Schistosomes.- Monoclonal Antibody Affinity Chromatography.- Immunoblotting and Enzyme-Linked Immunosorbent Assay.- Immunofluorescence of Parasites.- Immunoelectron Microscopic Localization of Antigens in Malaria Parasites.- Preparation of Blotted Membrane for Protein Microsequencing.- Identification of T-Cell Epitopes.