RNA Isolation and Characterization Protocols

"This book is aimed at clinical specialists as well as experimentalists...The references are recent. I strongly recommend its use by those involved in breast cancer treatment. It is one of the few books that deal with the management of benign breast conditions."-Oncology

• to Isolating RNA.- Large and Small Scale RNA Preparations from Eukaryotic Cells.- An Improved Rapid Method of Isolating RNA from Cultured Cells.- Isolating RNA with the Cationic Surfactant, Catrimox-14.- RNA Extraction from Formalin-Fixed and Paraffin-Embedded Tissues.- Extraction and Purification of RNA from Plant Tissue Enriched in Polysaccharides.- Isolation of Plant Mitochondrial RNA from Green Leaves.- Extraction of RNA from Fresh and Frozen Blood.- Isolation of Total RNA from Bacteria.- Isolation of Total RNA from Tissues or Cell Lines.- Isolation of Messenger RNA.- UV Spectrophotometric Analysis of Ribonucleic Acids.- Formaldehyde Gel Electrophoresis of Total RNA.- Preparation of RNA Dot-Blots.- Nonradioactive Northern Blotting.- The Use of RNA Probes for the Analysis of Gene Expression.- Analysis of RNA by Northern Blotting Using Riboprobes.- RNA Quantitative Analysis from Fixed and Paraffin-Embedded Tissues.- Quantitative Analysis of RNA Species by PCR and Solid-Phase Minisequencing.- Preparation of Tissue Sections and Slides for mRNA Hybridization.- Detecting mRNA in Tissue Sections with Digoxigenin-Labeled Probes.- One-Tube RT-PCR with Sequence-Specific Primers.- Identification of Differentially Expressed Genes by Nonradioactive Differential Display of Messenger RNA.- Characterization of RNA Using Continuous RT-PCR Coupled with ELOSA.- Gene Expression Analysis by CD-RT-PCR Eric de Kant.- Primer Extension Analysis of mRNA.- S1 Mapping Using Single-Stranded DNA Probes.- Measurements of Rate of Transcription in Isolated Nuclie by Nuclear “Run-Off” Assay.- Transcription In Vitro Using Bacteriophage RNA Polymerases.- In Vitro Translation of Messenger RNA in a Rabbit Reticulocyte Lysate Cell-Free System.- In Vitro Translation of Messenger RNA in a Wheat Germ Extract Cell-Free System.- The Xenopus Egg Extract Translation System.- Purification and Characterization of Viral dsRNA Genome Profiles by Crosshybridization.