Gene Probes

"In this book, the editors have brought together a comprehensive collection of time-tested protocols for designing and using gene probes in a wide variety of applications." -Clinical Laboratory International

• Target Format and Hybridization Conditions.- End Labeling Procedures.- Photobiotin Labeling.- Nick Translation.- Random Primed Labeling.- Design and Evaluation of 16S rRNA-Targeted Oligonucleotide Probes for Fluorescence In Situ Hybridization.- Evaluation of Alterations in the Tumor Suppressor Genes INK4A and INK4B in Human Bladder Tumors.- The ?-Tubulin Gene Region as a Molecular Marker to Distinguish Leishmania Parasites.- Detection of Malignant Plasma Cells in the Bone Marrow and Peripheral Blood of Patients with Multiple Myeloma.- Detection of Chlamydia trachomatis by DNA Amplification.- Isolation and Identification of Campylobacter Species by Use of Selective Enrichment, Nucleic Acid Amplification Methods, and Gene Probes.- Diagnosis of Human Papillomavirus Using In Situ Hybridization and In Situ Polymerase Chain Reaction.- Quantitation of In Situ Hybridization Using Image Analysis of Radioactively Labeled RNA Probes.- Stable Isotope Probing for Detection of Methanotrophs After Enrichment with 13CH4.- Detection of Enterococci in Freshwater and Seawater (16S and 23S rRNA Enterococcus Oligonucleotide Probes).- Analysis of 16S–23S rRNA Intergenic Spacer of Vibrio cholerae and Vibrio mimicus for Detection of These Species.- Colony Hybridization of Bacterial Isolates with Burkholderia cepacia-Specific Probes.- In Situ Hybridization of Burkholderia cepacia Cells.- Detection of Polychlorinated Biphenyl-Degrading Organisms in Soil.- Detection of Single-Copy Genes in DNA from Transgenic Plants.- Differentiation Between Transcripts of Genes Belonging to Small Families During Fruit Ripening and Abscission.- Polymerase Chain Reaction Detection of Invasive Shigella and Salmonella enterica in Food.- Polymerase Chain Reaction for Detection of Listeria monocytogenes.