Microwave Techniques and Protocols

AvRichard T. Giberson,Richard S. Demaree Jr.

Inbunden, Engelska, 2001

Del i serien Springer Protocols Handbooks

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Beskrivning

The contributors to this manual are uniformly from those labora- ries routinely using microwave technology to facilitate their processing methods in the various fields of microscopy. The methods and results these authors describe are the tangible evidence that microwaves can be used routinely as the basis for improved sample processing for micr- copy applications. These applications include complete sample proce- ing protocols for light and electron microscopy, decalcification, and immunocytochemistry. The overall time savings, ease of use, and qu- ity of results serve as justification for using microwaves in the labo- tory. The question as to whether there is a "microwave effect" is alluded to, but not discussed in any great detail. When the term microwave technology is used, it is generic and intended to mean equipment designed for laboratory versus household use. Microwave Techniques and Protocols is designed for anyone with a background and experience in sample processing for immunocytoch- istry, decalcification, light microscopy, or electron microscopy, and clearly demonstrates that microwave technology has a place in today's laboratory. Richard T. Giberson, Richard S.Demaree Jr. , REFERENCES Giberson, R. T. Demaree, R. S. , and (1995) Microwave fixation: understa- ing the variables to achieve rapid reproducible results. Microsc Res Tech 32:246-254. Giberson, R. T. , Demaree, R. S. , Jr. , and Nordhausen, R. W. (1997) Four-hour processing of clinical/diagnostic specimens for electron microscopy using microwave technique. J Vet Diagn Invest 9:61-67. Jr. VhD Ms vi Preface ...v Contributors ...

Produktinformation

Utgivningsdatum:2001-08-03
Mått:178 x 254 x 18 mm
Vikt:501 g
Format:Inbunden
Språk:Engelska
Serie:Springer Protocols Handbooks
Antal sidor:220
Förlag:Humana Press Inc.
ISBN:9780896039032

Utforska kategorier

Recensioner i media

"...if you study archived pathology sections, rat hippocampal slices, decalcification, sediments, Allium and Drosophilia nuclei, motor endplates or skin, there is something for you here. Highly specialized therefore, but invaluable if you need this procedure." -Ultramicroscopy "Microwave Techniques and Protocols is both informative, providing a broad perspective on how microwave treatment of samples is being used, and useful as a procedural manual for performing microwave sample treatment and for developing your own techniques." -Journal of Chemical Neuroanatomy

Innehållsförteckning

Overview of Microwave-Assisted Tissue Processing for Transmission Electron Microscopy.- Vacuum-Assisted Microwave Processing of Animal Tissues for Electron Microscopy.- Vacuum-Microwave Combination for Processing Plant Tissues for Electron Microscopy.- Basic Procedure for Electron Microscopy Processing and Staining in Clinical Laboratory Using Microwave Oven.- Specimen Preparation for Thin-Section Electron Microscopy Utilizing Microwave-Assisted Rapid Processing in a Veterinary Diagnostic Laboratory.- Microwave Processing of Archived Pathology Specimens for Ultrastructural Examination.- Microwave Fixation of Rat Hippocampal Slices.- Microwave Processing Techniques for Biological Samples in a Service Laboratory.- Microwave-Accelerated Decalcification.- Microwave Processing of Sediment Samples.- Microwave Polymerization in Thin Layers of London Resin White Allows Selection of Specimens for Immunogold Labeling.- In Vivo Microwave-Assisted Labeling of Allium and Drosophila Nuclei.- Microwave-Assisted Cytochemistry.- Microwave-Assisted Immunoelectron Microscopy of Skin.- Microwave Paraffin Techniques for Botanical Tissues.- Microwave-Assisted Formalin Fixation of Fresh Tissue.- Microwave-Assisted Processing of Biological Samples for Scanning Electron Microscopy.