David K. Gardner AM, FAA, DPhil is a Professor in the School of BioSciences at the University of Melbourne, and is the Scientific Director of Melbourne IVF, Melbourne, Australia.Ariel Weissman MD is a Senior Physician at the IVF Unit, Department of Obstetrics and Gynecology, Edith Wolfson Medical Center, Holon, and a full Professor of Obstetrics and Gynecology at the Sackler Faculty of Medicine, Tel Aviv University, Tel Aviv, Israel.Colin M. Howles PhD, FRSM is based at Aries Consulting SARL, Geneva, Switzerland, and is an Honorary Fellow, University of Edinburgh, UK.Zeev Shoham MD is a Professor at the Hebrew University, Jerusalem, Israel, a consultant in IVF, and co-founder of IVF-Worldwide.

• Preface. Contributors. Updated Guidelines for Setting up an Assisted Reproductive Technology Laboratory. Quality Control: Maintaining Stability in the Laboratory. KPIs for the IVF Laboratory. QC in the Clouds—Digitizing Quality Control. The Assisted Reproduction Technology Laboratory: Current Standards. Evaluation of Sperm. Sperm Preparation Techniques and Advanced Sperm Selection for Intracytoplasmic Sperm Injection. Sperm Chromatin Assessment. Oocyte Retrieval and Selection. Preparation and Evaluation of Oocytes for Intracytoplasmic Sperm Injection. Use of IVM in a Clinical Setting: What is its Role in ART?. Intracytoplasmic Sperm Injection: Technical Aspects. Human Embryo Biopsy Procedures. Analysis of Fertilization. Culture Systems for the Human Embryo. Embryo Selection through the Analysis of Morphology and Physiology. Evaluation of Embryo Quality: Time-Lapse Imaging to Assess Embryo Morphokinesis. Artificial Intelligence (AI). Artificial Intelligence (AI) in Gamete and Embryo Selection. Demystifying Vitrification. Vitrification of the Human Oocyte. The Human Embryo: Vitrification. Managing an Oocyte Bank. Severe Male Factor Infertility: Genetic Consequences and Recommendations for Genetic Testing. Pre-Implantation Genetic Testing for Aneuploidy to Improve Clinical Outcomes. Diagnosis of Endometrial Receptivity, Embryo–Endometrial Dialogue, and Endometrial Microbiome. Artificial Gametes: Oocytes. How Microfluidics and Microfabrication will Improve Diagnosis and Treatment in Human Art. Genome Editing in Human Reproduction. Designing Disaster Plans for IVF Laboratories. Early Human Embryo Development Revealed by Static Imaging. Index.