Tuhin Subhra Santra is an Associate professor in the Department of Engineering Design at Indian Institute of Technology Madras, India. Dr. Santra is a tenure track "Honorary Visiting Professor" at National Tsing Hua University" Taiwan from 2018 to 2023, and he was a "Visiting Professor" at University of Cambridge, UK in 2019. Dr. Santra received his PhD in Bio-NEMS from National Tsing Hua University (NTHU), Taiwan in 2013 and he was a post-doctoral researcher at the University of California, Los Angeles (UCLA), USA, from 2015 to 2016. His areas of main research interests include Bio-NEMS, MEMS, single-cell technologies, single-biomolecule detection, bio-micro/nano fabrication, biomedical micro/nano devices, nanomedicine, etc.Dr. Santra served as a Guest Editor for Frontiers of Bioengineeringand Biotechnology 2021, Cells, MDPI 2021, 2020; International Journal of Molecular Science (IJMS) 2018, 2017, 2015; Sensors Journal 2016; Journal of Molecules 2016; and Journal of Micromachines 2020, 2019, 2013, among others. Dr. Santra has received many honors and awards such as "Wellcome Trust/DBT India Alliance Fellowship" in 2018; Honorary Research Fellow from National Tsing Hua University, Taiwan in 2018; IEEE-NEMS best conference paper award in 2014; etc. Dr. Santra has published more than 45 international journals, has 25 US, Taiwan, and Indian pending/ approved patents, 8 books, 20 book chapters, 20 proceedings, in his research field.Fan-Gang Tseng Fan-Gang (Kevin) Tseng received his PhD in Microelectro Mechanical System (MEMS) from University of California, Los Angeles, USA (UCLA). He became an Assistant Professor in Department of Engineering and System Science, National Tsing Hua University (NTHU), Taiwan from August 1999. Currently, he is a Professor in the Department of Engineering and System Science (ESS). He is a Deputy Director of Biomedical Technology Research Center, NTHU, form 2009. He is also an affiliated Professor in Institute of Nanoengineering and Microsystem (NEMS), NTHU, and Research Fellow in Academia Sinica, Taiwan. Dr. Tseng focuses his research area in MEMS, Bio-MEMS, Nano-biotechnology, Nanomedicine, Fuel Cell, MEMS packaging and Integration. Dr. Tseng is in Editorial board of Applied Sciences from 2010, open Micromachine Journal 2009, etc. Dr. Tseng received many awards such as outstanding in research award from National Science Council, Taiwan 2010–2013, iCAN Taiwan competition award, Ton-Yuan innovative competition award, the best project award, NTHU outstanding in research award, 2005–2007, National innovation award, First class research award from National Research Council 2005–2008, outstanding teaching award 2003, new faculty research award and many more. Dr. Tseng has published 8 books/book chapters, more than 40 US and Taiwan patents. Also he has published more than 200 international Journal papers and 350 International Conference paper/proceedings related to this field.

• Chapter-1Microfluidics-based DNA detectionHogi Hartanto, Tianyi Jiang, Chenyu Cui, Ting-Hsuan ChenChapter-2Single-molecule detection by solid-state nanopores Chun-Yen Lee, Amer Alizadeh, Hirofumi Daiguji, Wei-Lun HsuChapter-3Confocal microscope-based detectionXue Wang, Xinchao Lu, Chengjun HuangChapter-4 Flow cytometry-based detectionJames F. LearyChapter-5Single-molecule separation Ching-Te Kuo, Yu-Chia LinChapter-6Single-molecule and single-particle tracking and analysis in a living cell Yen-Liang LiuChapter-7Determining the location and movement of biomolecules and biomolecular complexes in single microbial cells E. O. PuchkovChapter-8Pull down assay-based protein analysisAmandeep Kaur, Satish Pandey, Suman SinghChapter-9Atomic force microscopy for single biomolecule analysis Kavitha Illath, Ashwini Shinde, Moeto Nagai, Tuhin Subhra SantraChapter-10SERS analysis for single-molecule detection of disease biomarkers Muthu Meenakshi M, Gowri Annasamy, Kaavya P, Hema Brindha M, Yih Bing Chu, Ashwin Kumar Chapter-11Single molecule imaging using state-of-the-art microscopy techniquesArun Bhupathi, Hema Brindha M, Ganapathy Krishnamurthi, Ashwin Kumar

Tuhin Subhra Santra is an Associate professor in the Department of Engineering Design at Indian Institute of Technology Madras, India. Dr. Santra is a tenure track "Honorary Visiting Professor" at National Tsing Hua University" Taiwan from 2018 to 2023, and he was a "Visiting Professor" at University of Cambridge, UK in 2019. Dr. Santra received his PhD in Bio-NEMS from National Tsing Hua University (NTHU), Taiwan in 2013 and he was a post-doctoral researcher at the University of California, Los Angeles (UCLA), USA, from 2015 to 2016. His areas of main research interests include Bio-NEMS, MEMS, single-cell technologies, single-biomolecule detection, bio-micro/nano fabrication, biomedical micro/nano devices, nanomedicine, etc.Dr. Santra served as a Guest Editor for Frontiers of Bioengineeringand Biotechnology 2021, Cells, MDPI 2021, 2020; International Journal of Molecular Science (IJMS) 2018, 2017, 2015; Sensors Journal 2016; Journal of Molecules 2016; and Journal of Micromachines 2020, 2019, 2013, among others. Dr. Santra has received many honors and awards such as "Wellcome Trust/DBT India Alliance Fellowship" in 2018; Honorary Research Fellow from National Tsing Hua University, Taiwan in 2018; IEEE-NEMS best conference paper award in 2014; etc. Dr. Santra has published more than 45 international journals, has 25 US, Taiwan, and Indian pending/ approved patents, 8 books, 20 book chapters, 20 proceedings, in his research field.Fan-Gang Tseng Fan-Gang (Kevin) Tseng received his PhD in Microelectro Mechanical System (MEMS) from University of California, Los Angeles, USA (UCLA). He became an Assistant Professor in Department of Engineering and System Science, National Tsing Hua University (NTHU), Taiwan from August 1999. Currently, he is a Professor in the Department of Engineering and System Science (ESS). He is a Deputy Director of Biomedical Technology Research Center, NTHU, form 2009. He is also an affiliated Professor in Institute of Nanoengineering and Microsystem (NEMS), NTHU, and Research Fellow in Academia Sinica, Taiwan. Dr. Tseng focuses his research area in MEMS, Bio-MEMS, Nano-biotechnology, Nanomedicine, Fuel Cell, MEMS packaging and Integration. Dr. Tseng is in Editorial board of Applied Sciences from 2010, open Micromachine Journal 2009, etc. Dr. Tseng received many awards such as outstanding in research award from National Science Council, Taiwan 2010–2013, iCAN Taiwan competition award, Ton-Yuan innovative competition award, the best project award, NTHU outstanding in research award, 2005–2007, National innovation award, First class research award from National Research Council 2005–2008, outstanding teaching award 2003, new faculty research award and many more. Dr. Tseng has published 8 books/book chapters, more than 40 US and Taiwan patents. Also he has published more than 200 international Journal papers and 350 International Conference paper/proceedings related to this field.

• Chapter-1Microfluidics-based DNA detectionHogi Hartanto, Tianyi Jiang, Chenyu Cui, Ting-Hsuan ChenChapter-2Single-molecule detection by solid-state nanopores Chun-Yen Lee, Amer Alizadeh, Hirofumi Daiguji, Wei-Lun HsuChapter-3Confocal microscope-based detectionXue Wang, Xinchao Lu, Chengjun HuangChapter-4 Flow cytometry-based detectionJames F. LearyChapter-5Single-molecule separation Ching-Te Kuo, Yu-Chia LinChapter-6Single-molecule and single-particle tracking and analysis in a living cell Yen-Liang LiuChapter-7Determining the location and movement of biomolecules and biomolecular complexes in single microbial cells E. O. PuchkovChapter-8Pull down assay-based protein analysisAmandeep Kaur, Satish Pandey, Suman SinghChapter-9Atomic force microscopy for single biomolecule analysis Kavitha Illath, Ashwini Shinde, Moeto Nagai, Tuhin Subhra SantraChapter-10SERS analysis for single-molecule detection of disease biomarkers Muthu Meenakshi M, Gowri Annasamy, Kaavya P, Hema Brindha M, Yih Bing Chu, Ashwin Kumar Chapter-11Single molecule imaging using state-of-the-art microscopy techniquesArun Bhupathi, Hema Brindha M, Ganapathy Krishnamurthi, Ashwin Kumar

Tuhin Subhra Santra is an Associate professor in the Department of Engineering Design at Indian Institute of Technology Madras, India. Dr. Santra is a tenure track "Honorary Visiting Professor" at National Tsing Hua University" Taiwan from 2018 to 2023, and he was a "Visiting Professor" at University of Cambridge, UK in 2019. Dr. Santra received his PhD in Bio-NEMS from National Tsing Hua University (NTHU), Taiwan in 2013 and he was a post-doctoral researcher at the University of California, Los Angeles (UCLA), USA, from 2015 to 2016. His areas of main research interests include Bio-NEMS, MEMS, single-cell technologies, single-biomolecule detection, bio-micro/nano fabrication, biomedical micro/nano devices, nanomedicine, etc.Dr. Santra served as a Guest Editor for Frontiers of Bioengineeringand Biotechnology 2021, Cells, MDPI 2021, 2020; International Journal of Molecular Science (IJMS) 2018, 2017, 2015; Sensors Journal 2016; Journal of Molecules 2016; and Journal of Micromachines 2020, 2019, 2013, among others. Dr. Santra has received many honors and awards such as "Wellcome Trust/DBT India Alliance Fellowship" in 2018; Honorary Research Fellow from National Tsing Hua University, Taiwan in 2018; IEEE-NEMS best conference paper award in 2014; etc. Dr. Santra has published more than 45 international journals, has 25 US, Taiwan, and Indian pending/ approved patents, 8 books, 20 book chapters, 20 proceedings, in his research field.Fan-Gang Tseng Fan-Gang (Kevin) Tseng received his PhD in Microelectro Mechanical System (MEMS) from University of California, Los Angeles, USA (UCLA). He became an Assistant Professor in Department of Engineering and System Science, National Tsing Hua University (NTHU), Taiwan from August 1999. Currently, he is a Professor in the Department of Engineering and System Science (ESS). He is a Deputy Director of Biomedical Technology Research Center, NTHU, form 2009. He is also an affiliated Professor in Institute of Nanoengineering and Microsystem (NEMS), NTHU, and Research Fellow in Academia Sinica, Taiwan. Dr. Tseng focuses his research area in MEMS, Bio-MEMS, Nano-biotechnology, Nanomedicine, Fuel Cell, MEMS packaging and Integration. Dr. Tseng is in Editorial board of Applied Sciences from 2010, open Micromachine Journal 2009, etc. Dr. Tseng received many awards such as outstanding in research award from National Science Council, Taiwan 2010–2013, iCAN Taiwan competition award, Ton-Yuan innovative competition award, the best project award, NTHU outstanding in research award, 2005–2007, National innovation award, First class research award from National Research Council 2005–2008, outstanding teaching award 2003, new faculty research award and many more. Dr. Tseng has published 8 books/book chapters, more than 40 US and Taiwan patents. Also he has published more than 200 international Journal papers and 350 International Conference paper/proceedings related to this field.

• Chapter-1Microfluidics-based DNA detectionHogi Hartanto, Tianyi Jiang, Chenyu Cui, Ting-Hsuan ChenChapter-2Single-molecule detection by solid-state nanopores Chun-Yen Lee, Amer Alizadeh, Hirofumi Daiguji, Wei-Lun HsuChapter-3Confocal microscope-based detectionXue Wang, Xinchao Lu, Chengjun HuangChapter-4 Flow cytometry-based detectionJames F. LearyChapter-5Single-molecule separation Ching-Te Kuo, Yu-Chia LinChapter-6Single-molecule and single-particle tracking and analysis in a living cell Yen-Liang LiuChapter-7Determining the location and movement of biomolecules and biomolecular complexes in single microbial cells E. O. PuchkovChapter-8Pull down assay-based protein analysisAmandeep Kaur, Satish Pandey, Suman SinghChapter-9Atomic force microscopy for single biomolecule analysis Kavitha Illath, Ashwini Shinde, Moeto Nagai, Tuhin Subhra SantraChapter-10SERS analysis for single-molecule detection of disease biomarkers Muthu Meenakshi M, Gowri Annasamy, Kaavya P, Hema Brindha M, Yih Bing Chu, Ashwin Kumar Chapter-11Single molecule imaging using state-of-the-art microscopy techniquesArun Bhupathi, Hema Brindha M, Ganapathy Krishnamurthi, Ashwin Kumar